Application of microbial extracellular carbohydrate polymeric substances in food and allied industries

Tiwari, Onkar Nath; Sasmal, Soumya; Kataria, Ajay Kumar; Devi, Indrama

doi:10.1007/s13205-020-02200-w

Cited by 38 publications

(14 citation statements)

References 98 publications

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“…Therefore, the depleted carbohydrate yield in these sequences is a possible consequence of prior deduction of EPS in the form of CPS, bound to the cell surface. EPS reported to be chiefly constituted of carbohydrates 69 provides a strong justification for this fact. Nevertheless, bioethanol being our key interest, any loss in the carbohydrate yield cannot be afforded as this would hinder the quantity of bioethanol produced.…”

Section: Discussionmentioning

confidence: 94%

A waste-to-wealth initiative exploiting the potential of Anabaena variabilis for designing an integrated biorefinery

Deb

Mallick

Bhadoria

2022

Sci Rep

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The current research work was an innovative approach providing dual advantages of waste bioremediation and an effective biorefinery. The study attempted to exploit wastewater like aqua discharge and solid wastes like poultry litter/cow dung for cyanobacterial cultivation. Aqua discharge appended with 7.5 g L−1 poultry litter turned out as the best combination generating 46% higher carbohydrate yield than BG-11 control. A. variabilis cultivation in this waste-utilized medium also revealed its excellent bioremediation ability. While 100% removal was observed for nitrite, nitrate, and orthophosphate, a respective 74% and 81% reduction was noted for ammonium and total organic carbon. Chemical and biological oxygen demands were also reduced by 90%. This work was also novel in developing a sequential design for the production of bioethanol and co-products like exopolysaccharides, sodium copper chlorophyllin, C-phycocyanin, and poly-β-hydroxybutyrate from the same cyanobacterial biomass. The developed biorefinery implementing the waste-utilized medium was one of its kind, enabling biomass valorization of 61%. Therefore, the present study would provide a leading-edge for tackling the high production costs that limit the practical viability of biorefinery projects. The recyclability of the bioremediated wastewater would not only curtail freshwater usage, the waste disposal concerns would also be mitigated to a great extent.

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Section: Discussionmentioning

confidence: 94%

A waste-to-wealth initiative exploiting the potential of Anabaena variabilis for designing an integrated biorefinery

Deb

Mallick

Bhadoria

2022

Sci Rep

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“…The composition is very complex and is mainly composed of polysaccharides, proteins, humus, nucleic acids, and lipids [37]. With the development of the food industry in recent years, it has been discovered that extracellular polymers are related to the properties that promote cell adhesion to surfaces, such as from drying or desiccation in bacteria growing in terrestrial habitats or from attack by antimicrobial agents [38]. In addition, EPS can fix heavy metals in the environment, thus causing heavy metal pollution in the host [39].…”

Section: Effect Of Pf-1240 On the Eps Of H Alveimentioning

confidence: 99%

Quorum Quenching Enzyme (PF-1240) Capable to Degrade AHLs as a Candidate for Inhibiting Quorum Sensing in Food Spoilage Bacterium Hafnia alvei

Shen

Cui

Wang

et al. 2021

Foods

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Quorum sensing (QS) is widely present in microorganisms in marine aquatic products. Owing to the use of antibiotics, many spoilage bacteria in aquatic products are drug resistant. In order to slow down this evolutionary trend, the inhibition of spoilage phenotype of spoilage bacteria by interfering with QS has become a research hot spot in recent years. In this study, we found a new QS quenching enzyme, PF-1240; it was cloned and expressed in Pseudomonas fluorescens 08. Sequence alignment showed that its similarity with N-homoserine lactone (AHL) acylase QuiP protein of Pseudomonas fluorescens (Pf 0-1) was 78.4%. SDS-PAGE confirmed that the protein is a dimer composed of two subunits, which is similar to the structure of AHL acylases. The concentration of heterologous expression in Escherichia coli (DE3) was 26.64 μg/mL. Unlike most AHL acylases, PF-1240 can quench AHLs with different carbon chain lengths and inhibit the quorum sensing of the aquatic spoilage bacterium Hafnia alvei. It can significantly reduce the formation rate of biofilm of H. alvei to 44.4% and the yield of siderophores to 54%, inhibit the production of protease and lipase, and interfere with the motility of H. alvei. Through these corruption phenotypes, the specific application effect of PF-1240 can be further determined to provide a theoretical basis for its application in the preservation of practical aquatic products.

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“…They have excellent biocompatibility, renewability, biodegradability, easy availability, stable production cost, constant and reproducible physiological properties [11]. Due to the unique physical properties that only bacterial polysaccharides can possess, they have emerged as a biomaterial that has recently been in the spotlight [12][13][14][15]. Bacterial polysaccharides can generally be divided into three groups: intracellular polysaccharides, cell wall polysaccharides, and extracellular polysaccharides.…”

Section: Introductionmentioning

confidence: 99%

Bacterial Succinoglycans: Structure, Physical Properties, and Applications

Jeong

Kim

et al. 2022

Polymers

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Succinoglycan is a type of bacterial anionic exopolysaccharide produced from Rhizobium, Agrobacterium, and other soil bacteria. The exact structure of succinoglycan depends in part on the type of bacterial strain, and the final production yield also depends on the medium composition, culture conditions, and genotype of each strain. Various bacterial polysaccharides, such as cellulose, xanthan, gellan, and pullulan, that can be mass-produced for biotechnology are being actively studied. However, in the case of succinoglycan, a bacterial polysaccharide, relatively few reports on production strains or chemical and structural characteristics have been published. Physical properties of succinoglycan, a non-Newtonian and shear thinning fluid, have been reported according to the ratio of substituents (pyruvyl, succinyl, acetyl group), molecular weight (Mw), and measurement conditions (concentration, temperature, pH, metal ion, etc.). Due to its unique rheological properties, succinoglycan has been mainly used as a thickener and emulsifier in the cosmetic and food industries. However, in recent reports, succinoglycan and its derivatives have been used as functional biomaterials, e.g., in stimuli-responsive drug delivery systems, therapeutics, and cell culture scaffolds. This suggests a new and expanded application of succinoglycan as promising biomaterials in biomedical fields, such as tissue engineering, regenerative medicine, and pharmaceuticals using drug delivery.

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Application of microbial extracellular carbohydrate polymeric substances in food and allied industries

Cited by 38 publications

References 98 publications

A waste-to-wealth initiative exploiting the potential of Anabaena variabilis for designing an integrated biorefinery

A waste-to-wealth initiative exploiting the potential of Anabaena variabilis for designing an integrated biorefinery

Quorum Quenching Enzyme (PF-1240) Capable to Degrade AHLs as a Candidate for Inhibiting Quorum Sensing in Food Spoilage Bacterium Hafnia alvei

Bacterial Succinoglycans: Structure, Physical Properties, and Applications

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