Application of microfluidic technology based on surface-enhanced Raman scattering in cancer biomarker detection: A review

Nie, Changhong; Shaw, Ibrahim; Chen, Chuanpin

doi:10.1016/j.jpha.2023.08.009

Cited by 18 publications

(7 citation statements)

References 314 publications

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“…The use of SERS biosensors to detect exosomal miRNA associated with early stage lung cancer uses immobilized gold nanoparticles which are coated with capture probes for the specific miRNA on a chip; the SERS signal amplifies allowing for sensitive and specific detection. 177 Surface plasmon resonance detection (SPR) measures changes in the refractive index of a gold biosensor surface once the exosomes bind to an immobilized capture molecule. This is a real-time, label-free technique that provides high sensitivity and specificity.…”

Section: Application Of Nanotechnology In Exosomes-based Sensor Devel...mentioning

confidence: 99%

“…Each of these exosome molecules leaves a unique fingerprint; the Raman spectra are measured and analyzed by comparing the spectra to a reference from a library used to identify the exosomes and the biomarkers of the potential disease. The use of SERS biosensors to detect exosomal miRNA associated with early stage lung cancer uses immobilized gold nanoparticles which are coated with capture probes for the specific miRNA on a chip; the SERS signal amplifies allowing for sensitive and specific detection . Surface plasmon resonance detection (SPR) measures changes in the refractive index of a gold biosensor surface once the exosomes bind to an immobilized capture molecule.…”

Section: Application Of Nanotechnology In Exosomes Isolation and Dete...mentioning

confidence: 99%

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Fusion of Exosomes and Nanotechnology: Cutting-Edge Cancer Theranostics

Agnihotram,

Dhar,

Dhar

et al. 2024

ACS Appl. Nano Mater.

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Cancer is the most challenging health complication worldwide. Scientific society has tried to decode its complications and is developing a smart solution for mankind. Extracellular vesicles (EVs) are an emerging frontier in cancer research. EVs provide fresh insight into the understanding of cancer and are released from all the active cells. EVs are classified into some major classes, such as apoptotic bodies, microvesicles, and exosomes. Exosomes are among the most exciting tools in current cancer research as they work as cellular messengers (which reflects the cell's healthy or pathological conditional status). In the tumor microenvironment, they regulate several complicated events such as uncontrolled cell growth, immune suppression, angiogenesis, extracellular matrix remodeling, metastasis, organ-specific metastasis, drug and therapeutic resistance, and cancer stem cell development. Exosomes are also a source of promising cancer biomarkers involved in both diagnosis and prognosis and also performs a therapeutic role. Exosomes-based cancer nanomedicine which is application of nanosize vesicles in theranostics and sensor orientation, supports in overcoming several cancer management limitations, such as early diagnosis and therapeutics as they are specific, effective, and affordable. In this review, we have highlighted the integration of exosomes and nanotechnology which is a cutting-edge approach in precision oncology.

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Section: Application Of Nanotechnology In Exosomes-based Sensor Devel...mentioning

confidence: 99%

Section: Application Of Nanotechnology In Exosomes Isolation and Dete...mentioning

confidence: 99%

Fusion of Exosomes and Nanotechnology: Cutting-Edge Cancer Theranostics

Agnihotram,

Dhar,

Dhar

et al. 2024

ACS Appl. Nano Mater.

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“…23,24 These advantages make SERS attractive in the field of (micro)biology. 25,26 It is important to understand that SERS does not amplify all molecular bonds equally in complex biological systems such as cells. Consequently, SERS does not characterize the entire system.…”

Section: Introductionmentioning

confidence: 99%

Label-Free Surface-Enhanced Raman Scattering-Based Microbial Whole-Cell Biosensor for Acute Toxicity Assessment in Water

Ma,

Liu,

Zhou

et al. 2024

ACS EST Water

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Aquatic toxicity assessment is extremely important to ensuring water safety. Typically, the evaluation was accomplished by introducing bioluminescent bacteria, where the weakened luminescence was used to calculate the acute toxicity. Herein, a new microbial wholecell biosensor was proposed for aquatic toxicity evaluation using a labelfree surface-enhanced Raman spectroscopy (SERS) technique with Escherichia coli (E. coli) as a model organism. The proposed strategy required neither special bacteria strains nor mediators. The dosedependent SERS signal of the exposed model bacteria can be directly acquired. For the model toxic chemical Cu 2+ , the half-inhibitory concentration (IC 50 ) was found to be 3.87 mg/L. The method also helps understand the mechanism of toxicity of bacterial cells after exposure to a toxic environment, providing a multiparameter view of the changes in bacterial physiological metabolism induced by toxicants.

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“…Point-of-care testing (POCT), which involves real-time analysis near or at the site of patient specimen collection, has widespread applications in the fields of healthcare diagnostics and environmental monitoring 1,2 due to its excellent convenience, cost-effectiveness, specificity, accuracy, portability and low operation requirements. [3][4][5] Generally, emergent POCT systems use various biosensing methods with optical and electro-chemical readouts to provide quantitative results, 3,6 such as electrochemistry, 7 colorimetry, 8 electrogenerated chemiluminescence (ECL), 3,9,10 surface-enhanced Raman scattering, 11 fluorescence (FL), 12 etc. Among them, ECL is an ideal approach for POCT because of its advantages of high sensitivity at low concentrations, high spatiotemporal controllability, and the fact that it does not require an external light source.…”

Section: Introductionmentioning

confidence: 99%