Application of molecular biological techniques for detection of epizootic hemorrhagic disease virus (EHDV-318) recovered from a sentinel calf in central Sudan

Mohammed, Muzaffar-Ur-Rehman; Aradaib, Imadeldin E.; Mukhtar, Maowia M.; Ghalib, H. W.; Riemann, Hans; Oyejide, A.; Osburn, Bennie

doi:10.1016/s0378-1135(96)00073-9

Cited by 19 publications

(6 citation statements)

References 11 publications

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“…However, in focal areas of endemicity, goats and cattle develop subclinical infection [6,8,10]. In our laboratory, a lot of research efforts have been made to facilitate rapid molecular detection and differentiation of orbiviruses, including BTV, in susceptible native breeds of livestock [11,13,15,17,24,27-33]. …”

Section: Discussionmentioning

confidence: 99%

“…Therefore, it is becoming increasingly obvious that the control of orbiviruses, particularly BTV, is especially important in the Sudan given the large numbers of livestock in the country, and their importance to the national economy and rural communities. The epidemiologic studies including implementation of improved surveillance are urgently needed to better predict and respond to this devastating disease in the Sudan [24]. Most of the existing data about the epidemiology of BTV in the Sudan are relatively old.…”

Section: Introductionmentioning

confidence: 99%

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Prevalence of bluetongue virus infection and associated risk factors among cattle in North Kordufan State, Western Sudan

et al. 2014

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BackgroundBluetongue virus causes febrile disease in sheep and a fatal hemorrhagic infection in North American White-tailed deer. However, in cattle the disease is typically asymptomatic and no clinical overt disease is associated with bluetongue infection. Bluetongue virus activity has been detected in Khartoum, Sennar and South Darfur states of the Sudan. Currently, no information is available in regard to previous exposure of livestock to Bluetongue virus in North Kordufan State, the largest livestock producing region in the country. The present study was conducted to determine the prevalence of bluetongue antibodies and to identify the potential risk factors associated with the presence of bluetongue antibodies among cattle in North Kordufan State, Sudan. A total of 299 bovine blood samples were collected randomly from six localities in North Kordufan State and were tested by enzyme-linked immunosorbent assay (ELISA) for detection of BTV-specific immunoglobulin G (IgG) antibodies.ResultsThe serological evidence of Bluetongue virus infection was observed in 58 out of 299 cows, accounting for a 19.4% prevalence rate among cattle in North Kordufan State. Older cattle (>2 years of age) had four times the odds to be infected with BTV compared to young cattle (OR = 4.309, CI = 1.941-9.567, p-value = 0.01). Application of preventive measures, such as spraying or dipping with insecticide protects cattle against Bluetongue infection. Application of vector control measures decreased the odds for bluetongue seropositivity by 7 times (OR = 7.408, CI = 3.111-17.637, p-value = 0.01).ConclusionsThe results of this study indicated that age and application of routine insecticides are influential risk factors for seroprevalence of Bluetongue in cattle. Surveillance of Bluetongue virus should be extended to include other susceptible animals and to study the distribution of the insect vectors in the region to better predict and respond to BTV outbreak in the State of North Kordufan, Sudan.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Prevalence of bluetongue virus infection and associated risk factors among cattle in North Kordufan State, Western Sudan

et al. 2014

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“…EHDV serotypes 5, 6, 7 and 8 are reported in Australia (Allison et al , 2010; St George et al , 1983). EHDV-6 has been identified in Bahrain, the Sultanate of Oman, Sudan, Morocco and Algeria (Mohammed et al , 1996). Historically, in the USA only two serotypes, EHDV-1 (New Jersey strain) and EHDV-2 (Alberta strain), were identified (Allison et al , 2010).…”

Section: Introductionmentioning

confidence: 99%

Whole genome analysis of epizootic hemorrhagic disease virus identified limited genome constellations and preferential reassortment

Anbalagan¹,

Cooper²,

Klumper³

et al. 2014

Journal of General Virology

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Epizootic hemorrhagic disease virus (EHDV) is a Culicoides transmitted orbivirus that causes haemorrhagic disease in wild and domestic ruminants. A collection of 44 EHDV isolated from 2008 to 2012 was fully sequenced and analysed phylogenetically. Serotype 2 viruses were the dominant serotype all years except 2012 when serotype 6 viruses represented 63 % of the isolates. High genetic similarity (.94 % identity) between serotype 1 and 2 virus VP1, VP3, VP4, VP6, NS1, NS2 and NS3 segments prevented identification of reassortment events for these segments. Additionally, there was little genetic diversity (.96 % identity) within serotypes for VP2, VP5 and VP7. Preferential reassortment within the homologous serotype was observed for VP2, VP5 and VP7 segments for type 1 and type 2 viruses. In contrast, type 6 viruses were all reassortants containing VP2 and VP5 derived from an exotic type 6 with the remaining segments most similar to type 2 viruses. These results suggest that reassortment between type 1 and type 2 viruses requires conservation of the VP2, VP5 and VP7 segment constellation while type 6 viruses only require VP2 and VP5 and are restricted to type 2-lineage VP7. As type 6 VP2 and VP5 segments were exclusively identified in viruses with type 2-derived VP7, these results suggest functional complementation between type 2 and type 6 VP7 proteins.

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“…(Mohammed et al, 1996). Nevertheless, a few reports have shown that the virus may also cause clinical haemorrhagic disease in North American cattle (Metcalf et al, 1991).…”

Section: Resultsmentioning

confidence: 99%

“…This is because the samples were tested once instead of individual testing against each individual serotype of EHDV serogroup. Since the EHDV PCR assays are extremely sensitive procedures, the point should be stressed that PCR-negative animals would more than ful¢l the present requirements for negative status for the purpose of export (Mohammed et al, 1996;Aradaib et al, 2003).…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Epizootic Haemorrhagic Disease Virus Infection in Sentinel Calves from the San Joaquin Valley of California

2005

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Application of molecular biological techniques for detection of epizootic hemorrhagic disease virus (EHDV-318) recovered from a sentinel calf in central Sudan

Cited by 19 publications

References 11 publications

Prevalence of bluetongue virus infection and associated risk factors among cattle in North Kordufan State, Western Sudan

Prevalence of bluetongue virus infection and associated risk factors among cattle in North Kordufan State, Western Sudan

Whole genome analysis of epizootic hemorrhagic disease virus identified limited genome constellations and preferential reassortment

Evaluation of Epizootic Haemorrhagic Disease Virus Infection in Sentinel Calves from the San Joaquin Valley of California

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