Application of Nanocomposites from Bees Products and Nano-Selenium in Edible Coating for Catfish Fillets Biopreservation

Abstract

Bee products, e.g., chitosan and propolis (Pro), have extraordinary importance in many disciplines including food biopreservation. Fish meat is highly susceptible to vast spoilage, especially catfish (Clarias gariepinus) products. The current work involved the extraction of bees’ chitosan nanoparticles (BCht), Pro, Pro-mediated SeNPs and their composites, to evaluate them as potential antimicrobial and preservative nano-compounds, for the preservation of catfish fillets and augment their quality. BCht was extracted from bees (Apis mellifera) corpses and had a 151.9 nm mean particle diameter. The Pro was used for biosynthesis of SeNPs, which had 11.2 nm mean diameters. The entire compounds/composites exhibited powerful antibacterial acts against Escherichia coli, Staphylococcus aureus and Salmonella typhimurium, where S aureus had the uppermost resistance. BCht/Pro/SeNPs were the most forceful toward all bacterial strains. The constructed edible coatings (ECs) from produced compounds/composites (BCht, Pro, Pro/SeNPs, Pro/BCht and BCht/Pro/SeNPs) had elevated efficiency for preserving catfish fillets during cold storages for 7 days. The microbiological (total counts, psychrophilic bacteria, yeast and molds), spoilage chemical parameters (TVB-N, TBARS) and sensorial attributes (appearance, odor, color, overall quality) of ECs-treated fillets indicated the nanocomposite's efficiency for protecting the fish from microbial growth, the progress of chemical spoilage indicators and maintaining the sensorial quality of treated stored fillets. The most effective nanocomposite for maintaining the entire fillet’s quality was the BCht/Pro/SeNP. The based ECs on BNCt, Pro/SeNPs and their nanocomposites could be endorsed for prospective employment in the biopreservation of various seafoods.

Shelf‐life extension of Pacific white shrimp (Litopenaeus vannamei) using sodium alginate/chitosan incorporated with cell‐free supernatant of Streptococcus thermophilus FUA 329 during cold storage

“…The thiobarbituric acid reactive substance (TBARS) determination method was slightly modified according to our previous studies (Farajzadeh et al., 2016; Youssef et al., 2022). The method is based on the great reactivity of the thiobarbituric acid with carbonyl compounds that may arise as a consequence of lipid oxidation in shrimp.…”

A Review of the Antibacterial, Fungicidal and Antiviral Properties of Selenium Nanoparticles

“…One of the ways to enhance the antibacterial and antifungal properties of SeNPs is their functionalization with enzymes and polymers during synthesis or inclusion in a polymer matrix. Examples of such components can be: arabinogalactan, propolis, lysozyme, bacterial cellulose, gelatin, reduced graphene oxide, chitosan, collagen and ε-poly-L-lysine [121,125,[168][169][170][171][172][173]. The addition of SeNPs to textile fiber significantly enhances their antimicrobial properties [122,174].…”

“…Se nanospheres are described in a significant portion of the studies [117,[121][122][123]168]. However, other forms of SeNPs are described in a number of works: nanowires, nanorods and nanotubes.…”

Antimicrobial Nanocomposites from Chitosan and Squash Synthesized Nano‐Selenium Eradicate Skin Pathogens

“…The SEM imaging (using JEOL, JSM IT100, Japan), was employed for characterization of size, structure and the surface morphology of nanocomposites (e. g. NCht/SqM/SeNPs), after mounting and gold/palladium coating of screened samples. [41,42]…”