Application of nanomaterials in plant regeneration of rice ( Oryza sativa L.)

Chutipaijit, Sutee; Sutjaritvorakul, Thanawat

doi:10.1016/j.matpr.2017.06.107

Cited by 12 publications

(7 citation statements)

References 17 publications

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“…However, greening and organogenesis drastically declined with increasing concentrations of NPs in the culture medium. Similarly, a previous study has reported that TiO 2 NPs (20 mg l −1 ) resulted in the highest regeneration percentage of rice calli; however, any further increase in concentration of NPs resulted in a decline in the regeneration frequency 27 . A further investigation into the influence of PHAgNPs on the expression of PGR responsive genes encoding five important plant growth hormones—auxin, cytokinin, gibberellic acid, ethylene and ABA 28 —revealed that at 5 mg l −1 PHAgNP concentration, the auxin and cytokinin expression levels were not significantly altered, whereas the ethylene and ABA responsive gene expression decreased, and the regeneration frequency in calli was enhanced.…”

Section: Discussionsupporting

confidence: 58%

“…RD49. Further supplementation with TiO 2 NPs (20 mg l −1 ) in the regeneration medium enhances the callus regeneration frequency by approximately 8% relative to the control; however ZnO NPs, compared with control treatment, do not significantly enhance plant regeneration 27 . Another study has demonstrated that supplementation with TiO 2 (50 mg l −1 ) enhances callus induction frequency by 4% and regeneration frequency by approximately 2% in Oryza sativa L. cv.…”

Section: Discussionmentioning

confidence: 90%

“…However, ZnO and TiO2 NPs at concentrations of 5 mg l −1 and 20 mg l −1 , respectively, resulted in the highest induction of calli from indica rice cv. RD49 27 . After transfer of the induced calli to regeneration medium, the effects of PHAgNPs differed.…”

Section: Discussionmentioning

confidence: 99%

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A comprehensive study of the hormetic influence of biosynthesized AgNPs on regenerating rice calli of indica cv. IR64

Manickavasagam

Pavan

Vasudevan

2019

Sci Rep

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Rice is one of the most widely cultivated crops worldwide; however, it is not amenable to genetic manipulations, owing to its poor response to tissue culture and regeneration in vitro . To improve its response to tissue culture, we evaluated the influence of biosynthesized silver nanoparticles on callus induction, regeneration and rhizogenesis in Indica rice cv. IR64. Silver nanoparticles were biosynthesized by using silver nitrate and Parthenium hysterophorus plant extract, and were characterized by UV-visible spectroscopy, Fourier-transform infrared spectroscopy, Transmission electron microscopy and X-ray diffraction. The biosynthesized silver nanoparticles (PHAgNPs), when supplemented in tissue culture medium, promoted callus induction frequency, callus regeneration and rhizogenesis at concentrations of 10 mg l −1 , 5 mg l −1 and 10 mg l −1 , respectively. Further examination of the endogenous hormonal levels in regenerating calli revealed that AgNPs enhanced regeneration by alleviating abscisic acid and ethylene levels in the plant tissue. The stimulatory influence eliciting the regeneration response was found to be optimal with the supplementation of 5 mg l −1 PHAgNPs in the regeneration medium; the malondialdehyde, proline and hydrogen peroxide levels were also lower than those in the control, thus suggesting improved antioxidant status. Our results indicated that biosynthesized PHAgNPs may have the potential to positively influence tissue culture of recalcitrant varieties.

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Section: Discussionsupporting

confidence: 58%

Section: Discussionmentioning

confidence: 90%

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A comprehensive study of the hormetic influence of biosynthesized AgNPs on regenerating rice calli of indica cv. IR64

Manickavasagam

Pavan

Vasudevan

2019

Sci Rep

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“…After 2-weeks of callus induction, the induced calli were transferred to sterilized filter paper (Whatman ™ No.1) in the dark condition under 25±2 °C for one week. Embryogenic callus formed were shifted on the regeneration media RGM (2.5 mg/L of BAP + 0.5 mg/L of Kin) with varying concentrations of ZnO-NPs and without ZnO-NPs as control [ 27 ]. The pH of the medium was adjusted to 5.6–5.8.…”

Section: Methodsmentioning

confidence: 99%

“…Sterilized seeds were transferred to the callus induction medium supplemented with different concentrations Of ZnO nanoparticles (10, 20, 30, 40, 50 mg/L). For the induction of callogenesis, MS media with 2.5 mg/L of 2, 4-D was utilized and augmented along with different concentration of ZnO-NPs (10-50mg/L), and without ZnO-NPs as control [27]. The pH of medium was adjusted to 5.6-5.8.…”

Section: Callus Inductionmentioning

confidence: 99%

Green fabricated zinc oxide nanoformulated media enhanced callus induction and regeneration dynamics of Panicum virgatum L.

et al. 2020

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The current study focuses on the usage of bio synthesized zinc oxide nanoparticles to increase the tissue culture efficiency of important forage grass Panicum virgatum. Zinc being a micronutrient enhanced the callogenesis and regeneration efficiency of Panicum virgatum at different concentrations. Here, we synthesized zinc oxide nanoparticles through Cymbopogon citratus leaves extract to evaluate the effect of zinc oxide nanoparticles on plant regeneration ability in switchgrass. X-ray diffraction (XRD) and attenuated total reflectance-Fourier transform infrared (ATR-FTIR) validate phase purity of green synthesize Zinc oxide nanoparticles whereas, electron microscopy (SEM) has illustrated the average size of particle 50±4 nm with hexagonal rod like shape. Energy dispersive spectroscopy X-ray (EDS) depicted major peaks of Zn (92.68%) while minor peaks refer to Oxygen (7.32%). ZnO-NPs demonstrated the incredibly promising results against callogenesis. Biosynthesized ZnO-NPs at optimum concentration showed very promising effect on plant regeneration ability. Both the explants, seeds and nodes showed dose dependent response and upon high doses exceeding 40 mg/L the results were recorded negative, whereas at 30 mg/ L both explants demonstrated 70% and 76% regeneration frequency. The results conclude that ZnO-NPs enhance the plant growth and development and tailored the nutritive properties at nano-scale. Furthermore, eco-friendly approach of ZnO-NPs synthesis is strongly believed to improve in vitro regeneration frequencies in several other monocot plants.

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Impact of Nanoparticles on Photosynthesizing Organisms and Their Use in Hybrid Structures with Some Components of Photosynthetic Apparatus

Jampílek

Kráľová

2019

Plant Nanobionics

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Application of nanomaterials in plant regeneration of rice ( Oryza sativa L.)

Cited by 12 publications

References 17 publications

A comprehensive study of the hormetic influence of biosynthesized AgNPs on regenerating rice calli of indica cv. IR64

A comprehensive study of the hormetic influence of biosynthesized AgNPs on regenerating rice calli of indica cv. IR64

Green fabricated zinc oxide nanoformulated media enhanced callus induction and regeneration dynamics of Panicum virgatum L.

Impact of Nanoparticles on Photosynthesizing Organisms and Their Use in Hybrid Structures with Some Components of Photosynthetic Apparatus

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