Application of NIR and MIR spectroscopy for rapid determination of antioxidant activity of Radix Scutellariae from different geographical regions

Li, Hua; He, Jingxia; Li, Fajie; Zhang, Zhijie; Li, Raorao; Su, Jianchun; Zhang, Jidan; Yang, Bin

doi:10.1002/pca.2602

Cited by 20 publications

(11 citation statements)

References 30 publications

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“…Geographical region is usually regarded as one of the most important factors that affects the quality of traditional Chinese medicines [35] , [36] , [37] . The traditional best cultivated region of herbs is called Daodi region in China [38] .…”

Section: Resultsmentioning

confidence: 99%

Investigating chemical features of Panax notoginseng based on integrating HPLC fingerprinting and determination of multiconstituents by single reference standard

Yang

Zhu

Zhang

et al. 2018

Journal of Ginseng Research

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BackgroundPanax notoginseng is a highly valued medicine and functional food, whose quality is considered to be influenced by the size, botanical parts, and growth environments.MethodsIn this study, a HPLC method integrating fingerprinting and determination of multiconstituents by single reference standard was established and adopted to investigate the chemical profiles and active constituent contents of 215 notoginseng samples with different sizes, from different botanical parts and geographical regions.ResultsChemical differences among main root, branch root, and rotten root were not distinct, while rhizome and fibrous root could be discriminated from other parts. The notoginseng samples from Wenshan Autonomous Prefecture and cities nearby were similar, whereas samples from cities far away were not. The contents of major active constituents in main root did not correlate with the market price.ConclusionThis study provided comprehensive chemical evidence for the rational usage of different parts, sizes, and growth regions of notoginseng in practice.

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Section: Resultsmentioning

confidence: 99%

Investigating chemical features of Panax notoginseng based on integrating HPLC fingerprinting and determination of multiconstituents by single reference standard

Yang

Zhu

Zhang

et al. 2018

Journal of Ginseng Research

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“…Vibrational spectroscopy in combination with chemometrics have been used to determine STE and RBA [18] due to its property as fingerprint [19]. The simultaneous determination of respected compounds in some extracts or natural products can be achieved by FTIR spectroscopy combined with PLS calibration [20,21].…”

Section: Introductionmentioning

confidence: 99%

Quantitative Analysisof Stevioside and Rebaudioside a in Steviarebaudiana Leaves Using Infrared Spectroscopy and Multivariate Calibration

Martono

Rohman

2019

Int J App Pharm

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Objective: The objective of this research was to develop Fourier transform infrared (FTIR) spectroscopy in combination with multivariate analysis of partial least square (PLS) regression for quantitative analysis of stevioside and rebaudioside A in S. rebaudiana leaves extract.Methods: Stevia rebaudiana leaves with various ages were obtained from several high hills in Central Java, Indonesia. The extract samples were scanned using FTIR spectrophotometer in wavenumbers region of 4000–650 cm-1. PLS calibration model was established by plotting the actual value of stevioside and rebaudioside A as determined by high-performance liquid chromatography (HPLC) and FTIR predicted value. The performance of PLS regression was evaluated using coefficient determination (R2), root mean square error of calibration (RMSEC), root mean square error of prediction (RMSEP).Results: PLS regression for stevioside determination was successfully established using the combined wavenumber region of 671–1450 and 3279-3301 cm-1. PLS regression revealed R2of 0.9952with RMSEC value of0.84%. Meanwhile, rebaudioside A was determined at wavenumber region of 921–1508 cm-1using normal spectra. PLS model revealed R2 and RMSEC of 0.9911 and 0.70%, respectively.Conclusion: FTIR spectroscopy in combination with multivariate analysis of PLS regression could be used as an alternative method for quantitative analysis ofstevioside and rebaudioside A in S. rebaudiana leaves.

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“…Near infrared spectrum (NIRS) is widely used as alternatives to wet chemistry procedures for qualitative and quantitative analysis, which can be a rapid and effective instrument to classify different origin of G. elata coupled with chemometrics; since it is fast, easy to handle and non-destructive, it has been applied to classify coffee varieties, milk power and Chinese herbs [ 11 , 12 , 13 ]. However, few studies explore the identification of the cultivation origins of G. elata .…”

Section: Introductionmentioning

confidence: 99%

Discrimination of Gastrodia elata from Different Geographical Origin for Quality Evaluation Using Newly-Build Near Infrared Spectrum Coupled with Multivariate Analysis

Zuo

Deng

2018

Molecules

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Discrimination of Gastrodia elata (G. elata) geographical origin is of great importance to pharmaceutical companies and consumers in China. this paper focuses on the feasibility of near infrared spectrum (NIRS) combined multivariate analysis as a rapid and non-destructive method to prove its fit for this purpose. Firstly, 16 batches of G. elata samples from four main-cultivation regions in China were quantified by traditional HPLC method. It showed that samples from different origins could not be efficiently differentiated by the contents of four phenolic compounds in this study. Secondly, the raw near infrared (NIR) spectra of those samples were acquired and two different pattern recognition techniques were used to classify the geographical origins. The results showed that with spectral transformation optimized, discriminant analysis (DA) provided 97% and 99% correct classification for the calibration and validation sets of samples from discriminating of four different main-cultivation regions, and provided 98% and 99% correct classifications for the calibration and validation sets of samples from eight different cities, respectively, which all performed better than the principal component analysis (PCA) method. Thirdly, as phenolic compounds content (PCC) is highly related with the quality of G. elata, synergy interval partial least squares (Si-PLS) was applied to build the PCC prediction model. The coefficient of determination for prediction (Rp2) of the Si-PLS model was 0.9209, and root mean square error for prediction (RMSEP) was 0.338. The two regions (4800 cm−1–5200 cm−1, and 5600 cm−1–6000 cm−1) selected by Si-PLS corresponded to the absorptions of aromatic ring in the basic phenolic structure. It can be concluded that NIR spectroscopy combined with PCA, DA and Si-PLS would be a potential tool to provide a reference for the quality control of G. elata.

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Application of NIR and MIR spectroscopy for rapid determination of antioxidant activity of Radix Scutellariae from different geographical regions

Abstract: The developed spectroscopic method can be successfully applied in high-throughput screening of the antioxidant capability of Radix Scutellariae samples. It can also be a viable and advantageous alternative to laborious chemical procedures.

Cited by 20 publications

References 30 publications

Investigating chemical features of Panax notoginseng based on integrating HPLC fingerprinting and determination of multiconstituents by single reference standard

Investigating chemical features of Panax notoginseng based on integrating HPLC fingerprinting and determination of multiconstituents by single reference standard

Quantitative Analysisof Stevioside and Rebaudioside a in Steviarebaudiana Leaves Using Infrared Spectroscopy and Multivariate Calibration

Discrimination of Gastrodia elata from Different Geographical Origin for Quality Evaluation Using Newly-Build Near Infrared Spectrum Coupled with Multivariate Analysis

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