Application of Paramagnetically Tagged Molecules for Magnetic Resonance Imaging of Biofilm Mass Transport Processes

Ramanan, B.; Holmes, William M.; Sloan, William T.; Phoenix, Vernon R.

doi:10.1128/aem.03016-09

Cited by 17 publications

(9 citation statements)

References 46 publications

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“…Hence, it is often applied to visualize biofilms within porous media (e.g., soils; [Hoskins et al, ]) as well as resolve transport phenomena (Baumann and Werth, ; Gjersing et al, ; Hornemann et al, ). In addition to transport of dissolved species (i.e., metal ions; McLean et al, ; Ramanan et al, and substrates; Renslow et al, ), nanoparticle transport, interaction with humic substances, and deposition in porous media systems was studied recently (Cuny et al, ).…”

Section: Established Biofilm Imaging Techniquesmentioning

confidence: 99%

Optical coherence tomography in biofilm research: A comprehensive review

Wagner

Horn

2017

Biotech & Bioengineering

150

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Imaging of biofilm systems is a prerequisite for a better understanding of both structure and its function. The review aims to critically discuss the use of optical coherence tomography (OCT) for the visualization of the biofilm structure as well as its dynamic behavior. A short overview on common and well-known, established imaging techniques for biofilms such as scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), Raman microscopy (RM), and magnetic resonance imaging (MRI) paves the way to imaging biofilms at the mesoscale, which is perfectly covered by means of OCT. Principle, resolution, imaging velocity, and limitations of OCT are subsequently presented and discussed in the context of biofilm applications. Examples are provided showing the strength of this technique with respect to the visualization of the mesoscopic biofilm structure as well as the estimation of flow profiles and shear rates. Common and new structural parameters derived from OCT datasets are presented. Additionally, the review shows the importance of OCT with respect to a better description of mechanical biofilm properties. Finally, the implementation of multi-dimensional OCT datasets in biofilm modelling is shown by several examples aiming on an improved understanding of mass transfer at the bulk-biofilm interface. Biotechnol. Bioeng. 2017;114: 1386-1402. © 2017 Wiley Periodicals, Inc.

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Section: Established Biofilm Imaging Techniquesmentioning

confidence: 99%

Optical coherence tomography in biofilm research: A comprehensive review

Wagner

Horn

2017

Biotech & Bioengineering

150

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“…[GdDTPA] 2− , often used as contrast agent in medical MRI (Li et al, 2007), is also an ideal example of a contrast agent for MRI studies of metal transport in a biofilm matrix (Ramanan et al, 2010). This complex did not decrease the A 0 and the T 2 decrease approximately corresponded to the values expected based on the [GdDTPA] 2− relaxivity measured in free solution (Figure 4).…”

Section: Interaction Of the Complexed Metals With The Granular Biofilmentioning

confidence: 64%

Iron, Cobalt, and Gadolinium Transport in Methanogenic Granules Measured by 3D Magnetic Resonance Imaging

Bartacek

Vergeldt

Máca

et al. 2016

Front. Environ. Sci.

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Description of processes such as bioaccumulation, bioavailability and biosorption of heavy metals in biofilm matrixes requires the quantification of their transport. This study shows 3D MRI measurements of the penetration of free (Fe 2+ , Co 2+ and Gd 3+ ) and complexed ([FeEDTA] 2− and [GdDTPA] 2− ) metal ions in a single methanogenic granule. Interactions (sorption or precipitation) between free metals and the biofilm matrix result in extreme shortening of the spin-spin relaxation time (T 2 ) and a decrease of the amplitude (A 0 ) of the MRI signal, which hampers the quantification of the metal concentration inside the granular sludge matrix. MRI images clearly showed the presence of distinct regions (dead or living biomass, cracks, and precipitates) in the granular matrix, which influenced the metal transport. For the free metal ions, a reactive barrier was formed that moved through the granule, especially in the case of Gd 3+ . Chelated metals penetrated faster and without reaction front. Diffusion of [GdDTPA] 2− could be quantified, revealing the course of its transport and the uneven (0.2-0.4 mmol·L −1 ) distribution of the final [GdDTPA] 2− concentration within the granular biofilm matrix at equilibrium.

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“…Characteristic parameters determined using CLSM were found to differ from those obtained using OCT. Ramanan et al (2010) showed that mass transport processes in biofilms could be imaged using a paramagnetic tag and magnetic resonance imaging specifically with a complex of gadolimium and diethylenetriamine pentaacetic acid (Gd-DPTA) in a phototrophic biofilm. This technique was used to show that Gd-DPTA was transported purely by diffusion in agar, but by diffusion and advection in the biofilm.…”

Section: Techniques and Analytic Methodsmentioning

confidence: 99%

Biological Fixed Film

Wesley¹,

Lerner²,

Kim³

et al. 2011

Water Environment Research

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Literature published during the year 2010 on the topic of biological fixed film including biofilm and bioreactors for the purpose of water and wastewater treatment has been reviewed herein. Literature published regarding microbial fuel cells has also been included. The review has been organized into the following sections: biofilm formation, adhesion, quorum sensing, and consortia; techniques and analytic methods; biofilm reactors and modeling; and microbial fuel cells.

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Application of Paramagnetically Tagged Molecules for Magnetic Resonance Imaging of Biofilm Mass Transport Processes

Cited by 17 publications

References 46 publications

Optical coherence tomography in biofilm research: A comprehensive review

Optical coherence tomography in biofilm research: A comprehensive review

Iron, Cobalt, and Gadolinium Transport in Methanogenic Granules Measured by 3D Magnetic Resonance Imaging

Biological Fixed Film

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