Application of response surface methodology for rapid chrysene biodegradation by newly isolated marine-derived fungus Cochliobolus lunatus strain CHR4D

Bhatt, Jwalant K.; Ghevariya, Chirag M.; Dudhagara, Dushyant R.; Rajpara, Rahul K.; Dave, Bharti P.

doi:10.1007/s12275-014-4137-6

Cited by 23 publications

(7 citation statements)

References 17 publications

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“…Several fungal genera Cochilobolus , Sclerotina , Neosartoria , Mycosphaerella , Trichosporon , Penicillium , Auricularia and Teratosphaeria were only found in oil contaminated seawater, of which Cochilobolus , Neosartoria , Penicillium and Trichosporon have been previously reported for hydrocarbon degradation potential 25,52–55 . Interestingly, in the oiled seawater, Candida and Rhodotorula reduced in abundance by 33 to 1% and 0.7 to null respectively, even though these genera are reported to be hydrocarbon degraders from terrestrial environment 53 . Non-oiled sediment represented a total of 19 fungal species from the metagenomic analysis.…”

Section: Discussionmentioning

confidence: 97%

Oil-Spill Triggered Shift in Indigenous Microbial Structure and Functional Dynamics in Different Marine Environmental Matrices

Neethu

Saravanakumar

Purvaja

et al. 2019

Sci Rep

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Microbial degradation has long been recognized as the key rescue mechanism in shaping the oil polluted marine environments and the role of indigenous populations or their functional genomics have never been explored from Indian marine environments, post an oil spill event. In the current study, high throughput metagenomic analysis, PLFA profiling and mass spectrophotometric analysis was performed in combination with metabolomics to capture signature variations among the microbial communities in sediment, water and laboratory enrichments. Contrary to the previous reports, the bloom of Pseudomonadales (specifically genus Acinetobacter) in oiled sediment and Methylococcales in oiled water outnumbered the relative abundance of Alcanivorax in response to hydrocarbon contamination. Overall enhancement of xenobiotic degradation was suggested by metabolomic analysis in sediment and water post the spill event and varying quantitative assemblage of enzymes were found to be involved in hydrocarbon utilization. Laboratory enrichments revealed the competitive advantage of sediment communities over the water communities although unique taxa belonging to the later were also found to be enriched under in vitro conditions. Simultaneous analysis of sediment and water in the study provided explicit evidences on existence of differential microbial community dynamics, offering insight into possibilities of formulating nature identical solutions for hydrocarbon pollution.

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Section: Discussionmentioning

confidence: 97%

Oil-Spill Triggered Shift in Indigenous Microbial Structure and Functional Dynamics in Different Marine Environmental Matrices

Neethu

Saravanakumar

Purvaja

et al. 2019

Sci Rep

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“…Solvent phase was reduced using rotary vacuum evaporator (Büchi R215, Switzerland). After evaporation of solvent, solid white crystals were redissolved in known amount of DCM prior to analysis by GC-MS as suggested by [8][9][10].…”

Section: Extraction and Estimation Of Residual Anthracenementioning

confidence: 99%

Plackett- Burman Design for the Screening of Media Component for Anthracene Degradation by Sphingobium Yanoikuyae Strain ANT3D

Rajpara¹

2016

IJMBR

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International Journal of Marine Biology and Research Open Access Research Article the variables can eliminate these limitations of "one factor at time" approach. Statistical method has several advantages as being rapid, reliable, less expensive, screening of number of variables, understanding of effect of variable at various coded level [3]. Numbers of statistical models are available amongst them Plackett-Burman (PB) and Response Surface Methodology (RSM) is most widely used statistical models. Two level fractional factorial designs (PB design) is used for the screening of the variable with small number of experimental runs instead of using more extensive factorial design, which would furnish more detail explanation [4]. The present paper discusses screening of suitable medium components for degradation of anthracene by Sphingobium yanoikuyae strain ANT3D using Plackett-Burman design. Materials and Methods Organism and Medium Multiple PAHs degrading isolate S. yanoikuyae strain ANT3D was selected for optimization of BH medium components as MgSO 4 , CaCl 2 , KH 2 PO 4 , K 2 HPO 4 , NH 4 NO 3 , and FeCl 3. The S. yanoikuyae strain ANT3D was routinely grown on BH medium amended with anthracene at concentration of 50 mg/ L and stored at 4°C until use [5]. Enrichment and isolation of PAHs degrading bacteria using selective media Soil samples were collected from crude oil contaminated saline sites near Gulf of Kutch, Jamnagar coast (latitude 22°34'17.50" °N, 70°10'53.00" °E, longitude) Gujarat, India. Isolation of PAHs degrading bacteria was executed by using various selective media and techniques as described below: Nagel and Andreesen's (NA) [6] and Bushnell-Hass (BH) [7] media were used for enrichment of PAHs degrading bacteria from crude oil polluted marine sediment samples. For this, 1g of polluted sediment samples were added to 10 mL BH medium in test tube and vortexed for 1 min.

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“…In the instance of bioremediation, C. lunatus was found to biodegrade crude oil, resulting in a 1.5 percent weight loss (of the crude) in one week, 2.1 percent weight loss in two weeks, and 4.7 percent weight loss in three weeks [ 100 , 200 ]. Similarly, the fungus C. lunatus strain CHR4D was isolated from crude oil-polluted shorelines in India, and it was determined that chrysene (C 18 H 12 ), a four-ringed high molecular weight polycyclic aromatic hydrocarbon, was degraded swiftly and effectively [ 201 ]. Surprisingly, after only four days, 93.10 percent of the chrysene was eliminated [ 201 ].…”

Section: In Bioremediation and Waste Biomass Valorizationmentioning

confidence: 99%

Diversity, Lifestyle, Genomics, and Their Functional Role of Cochliobolus, Bipolaris, and Curvularia Species in Environmental Remediation and Plant Growth Promotion under Biotic and Abiotic Stressors

Khan

Asaf

Ahmad

et al. 2023

JoF

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Cochliobolus, Bipolaris, and Curvularia genera contain various devastating plant pathogens that cause severe crop losses worldwide. The species belonging to these genera also perform a variety of diverse functions, including the remediation of environmental contaminations, beneficial phytohormone production, and maintaining their lifestyle as epiphytes, endophytes, and saprophytes. Recent research has revealed that despite their pathogenic nature, these fungi also play an intriguing role in agriculture. They act as phosphate solubilizers and produce phytohormones, such as indole acetic acid (IAA) and gibberellic acid (GAs), to accelerate the growth of various plants. Some species have also been reported to play a significant role in plant growth promotion during abiotic stresses, such as salinity stress, drought stress, heat stress, and heavy metal stress, as well as act as a biocontrol agent and a potential mycoherbicide. Similarly, these species have been reported in numerous industrial applications to produce different types of secondary metabolites and biotechnological products and possess a variety of biological properties, such as antibacterial, antileishmanial, cytotoxic, phytotoxic, and antioxidant activities. Additionally, some of the species have been utilized in the production of numerous valuable industrial enzymes and biotransformation, which has an impact on the growth of crops all over the world. However, the current literature is dispersed, and some of the key areas, such as taxonomy, phylogeny, genome sequencing, phytohormonal analysis, and diversity, are still being neglected in terms of the elucidation of its mechanisms, plant growth promotion, stress tolerance, and bioremediation. In this review, we highlighted the potential role, function, and diversity of Cochliobolus, Curvularia, and Bipolaris for improved utilization during environmental biotechnology.

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Application of response surface methodology for rapid chrysene biodegradation by newly isolated marine-derived fungus Cochliobolus lunatus strain CHR4D

Cited by 23 publications

References 17 publications

Oil-Spill Triggered Shift in Indigenous Microbial Structure and Functional Dynamics in Different Marine Environmental Matrices

Oil-Spill Triggered Shift in Indigenous Microbial Structure and Functional Dynamics in Different Marine Environmental Matrices

Plackett- Burman Design for the Screening of Media Component for Anthracene Degradation by Sphingobium Yanoikuyae Strain ANT3D

Diversity, Lifestyle, Genomics, and Their Functional Role of Cochliobolus, Bipolaris, and Curvularia Species in Environmental Remediation and Plant Growth Promotion under Biotic and Abiotic Stressors

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