Application of RP-HPLC method for the simultaneous determination of cetirizine in the presence of quinolones

Abstract

Background Present work describes a fast, simple, and sensitive procedure for the simultaneous determination of cetirizine in the presence of quinolones using diclofenac sodium as an internal standard. The present work was designed to analyze these compounds in pharmaceutical and clinical labs being economical for use. Results The mobile phase consisted of the simple composition of methanol, acetonitrile, and water in a ratio of 50:20:30 with a pH adjusted to 3.1 at a flow rate of 1 mL min−1. The UV detection was performed at 225 nm. The linearity was assessed over the range of 2.5–50 μg mL−1 for all drugs. The parameters such as accuracy, precision, linearity (>0.999), and sensitivity were satisfactory. Conclusion The method was equally applicable for formulation and human serum with recovery values between 95 and 105%. The results of the method were validated statistically according to ICH guidelines.

Factorial design-assisted reverse phase HPLC–UV approach for the concurrent estimation of cetirizine and azelastine in aqueous humor

“…Our procedure had a shorter retention time for both drugs than the reported HPLC procedures 12 , 13 , 16 , 27 , 30 . The developed HPLC procedure, whose mobile phase is made up of only two components, is much simpler than other documented HPLC procedures 14 , 15 , 30 , 31 , whose mobile phases are made up of three or more components, therefore requiring more time for preconditioning. Also, our procedure had a lower LOQ for both drugs than the documented procedures 13 – 16 , 28 , 29 .…”

“…BP 8 stated non-aqueous potentiometric titration with sodium hydroxide, whilst USP 10 described high performance liquid chromatography (HPLC) procedure for CTZ estimation. Various analytical techniques have been developed for the determination of CTZ either in pharmaceutical preparations, human plasma or aqueous humor as HPLC [11][12][13][14][15][16] , capillary electrophoresis 17,18 , spectrofluorimetry [19][20][21][22][23] and spectrophotometry 24,25 .…”

Quantitation of Ciprofloxacin by RP-HPLC from Active and Dosage Formulations

“…Many methods have already been reported for estimations of ciprofloxacin [6][7][8][9][10][11][12] and but the present method is straightforward and is validated according to ICH (International Conference on Harmonization) guidelines [16]. All of the parameters were found under the limits of ICH guidelines, and the chromatogram of the analyte revealed that the method was specific and no peak of internal standard interfered, and can be considered selective.…”

“…suppositories, gels, eye drops, etc.) [6][7][8][9][10][11][12]. A direct and sensitive HPLC method for determining ciprofloxacin in the influenza vaccine has been described [7] employing a mobile phase of acetonitrile/water/phosphoric acid.…”

Synchronous Fluorescence as a Green and Selective Method for the Simultaneous Determination of Cetirizine and Azelastine in Aqueous Humor

“…Gradient elution Water: formic acid 0.13% (solvent A) and methanol (solvent B) 2. Expensive detector [ 8 ] HPLC–UV Pharmaceutical preparations 1–20 µg mL −1 1.0 µg mL −1 Acetonitrile: phosphate buffer pH 3.5 (40: 60 v/v) [ 9 ] HPLC–UV Pharmaceutical preparations 5–50 µg mL −1 1.9 µg mL −1 Methanol: phosphate buffer pH 3.5 (80: 20 v/v) [ 10 ] HPLC–UV Pharmaceutical preparations and human serum 2.5–50 µg mL −1 0.035 µg mL −1 Methanol: acetonitrile: water pH 3.1 (50: 20: 30 v/v/v) [ 11 ] HPLC–UV Human serum 1–100 µg mL −1 2.0 µg mL −1 Methanol: water pH 2.8 (70: 30 v/v) [ 12 ] Spectrofluorimetry Pharmaceutical preparation and human plasma 40–400 ng mL −1 8.3 ng mL −1 1. Reaction with potassium persulphate and 2-cynoacetamide in alkaline medium 2.…”