2024
DOI: 10.17816/gc635661
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Application of semi-quantitative loop-mediated isothermal amplification for gene expression study in Expi293 cells.

Pavel A. Bobrovsky,
Ekaterina N. Grafskaia,
Daria D. Kharlampieva
et al.

Abstract: BACKGROUND: Mammalian cell cultures play a key role in the pharmaceutical industry, requiring constant monitoring of the cell conditions during fermentation. In addition to monitoring of the physical-chemical parameters, it is important to evaluate the internal state of cells, for which gene expression analysis is used. Currently, quantitative reverse transcription polymerase chain reaction (qPCR) is the dominant method. However, the loop-mediated isothermal amplification (LAMP) technique also attracts attenti… Show more

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