Application of Semiconductor Quantum Dots for Breast Cancer Cell Sensing

Xu, Hengyi; Wei, Hua; Aguilar, Zoraida P.; Waldron, Jamie; Wang, Y. Andrew

doi:10.1109/bmei.2009.5305551

Cited by 6 publications

(6 citation statements)

References 25 publications

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“…The IOs being evaluated in this study are small (<20 nm), stable, water soluble, and consist of an amphiphilic polymer coating. The surface layer contains carboxylate groups that are readily available for conjugation with proteins, peptides, and DNA (25–27).…”

mentioning

confidence: 99%

Iron oxide nanoparticles as a clinically acceptable delivery platform for a recombinant blood‐stage human malaria vaccine

et al. 2012

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This study explored the novel use of iron oxide (IO) nanoparticles (<20 nm) as a vaccine delivery platform without additional adjuvants. A recombinant malaria vaccine antigen, the merozoite surface protein 1 (rMSP1), was conjugated to IO nanoparticles (rMSP1-IO). Immunizations in outbred mice with rMSP1-IO achieved 100% responsiveness with antibody titers comparable to those obtained with rMSP1 formulated with a clinically acceptable adjuvant, Montanide ISA51 (2.7×10 vs. 1.6×10; respectively). Only rMSP1-1O could induce significant levels (80%) of parasite inhibitory antibodies. The rMSP1-IO was highly stable at 4°C and was amenable to lyophilization, maintaining its antigenicity, immunogenicity, and ability to induce inhibitory antibodies. Further testing in nonhuman primates, Aotus monkeys, also elicited 100% immune responsiveness and high levels of parasite inhibitory antibodies (55-100% inhibition). No apparent local or systemic toxicity was associated with IO immunizations. Murine macrophages and dendritic cells efficiently (>90%) internalized IO nanoparticles, but only the latter were significantly activated, with elevated expression/secretion of CD86, cytokines (IL-6, TNF-α, IL1-b, IFN-γ, and IL-12), and chemokines (CXCL1, CXCL2, CCL2, CCL3, CCL4, and CXCL10). Thus, the IO nanoparticles is a novel, safe, and effective vaccine platform, with built-in adjuvancy, that is highly stable and field deployable for cost-effective vaccine delivery.

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confidence: 99%

Iron oxide nanoparticles as a clinically acceptable delivery platform for a recombinant blood‐stage human malaria vaccine

et al. 2012

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“…Quantum dots were also applied for breast cancer cell detection, utilizing a trio of antibodies: anti-HER2/neu for capturing cells on silanized glass, anti-EpCAM for labeling, and a third antibody against anti-EpCAM conjugated to QDs for imaging. This method produced bright, fluorescent signals of captured SK-BR3 cells under a UV microscope, highlighting the enhanced potential of QDs in cell sensing and imaging for multiplexed immunological assays [86].…”

Section: Quantum Dot Applications In Hormone and Protein Biomarker De...mentioning

confidence: 88%

Quantum Dot Research in Breast Cancer: Challenges and Prospects

Omidian,

Wilson,

Cubeddu

2024

Materials

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The multifaceted role of quantum dots (QDs) in breast cancer research highlights significant advancements in diagnostics, targeted therapy, and drug delivery systems. This comprehensive review addresses the development of precise imaging techniques for early cancer detection and the use of QDs in enhancing the specificity of therapeutic delivery, particularly in challenging cases like triple-negative breast cancer (TNBC). The paper also discusses the critical understanding of QDs’ interactions with cancer cells, offering insights into their potential for inducing cytotoxic effects and facilitating gene therapy. Limitations such as biocompatibility, toxicity concerns, and the transition from laboratory to clinical practice are critically analyzed. Future directions emphasize safer, non-toxic QD development, improved targeting mechanisms, and the integration of QDs into personalized medicine, aiming to overcome the current challenges and enhance breast cancer management.

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“…QDs are small (<15nm) inorganic nanoparticles with a crystal shell of alternating cationic and anionic layers, which in this case is CdSe/ZnS [37–39]. QDs are non-immunogenic, stable, and when coated with an organic layer allow for an array of proteins, DNA, and other biomolecules to be conjugated to their surfaces [37–39]. Because of their small size and surface modification, QDs are highly soluble and behave as a true solution [40].…”

Section: Introductionmentioning

confidence: 99%

Blood stage merozoite surface protein conjugated to nanoparticles induce potent parasite inhibitory antibodies

Pusic

Stridiron

et al. 2011

Vaccine

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In this proof-of-concept study we report the use of <15nm, water soluble, inorganic nanoparticles as a vaccine delivery system for a blood stage malaria vaccine. The recombinant malarial antigen, Merozoite Surface Protein 1 (rMSP1) of P. falciparum served as the model vaccine. The rMSP1 was covalently conjugated to polymer-coated quantum dot CdSe/ZnS nanoparticles (QDs) via surface carboxyl groups, forming rMSP1-QDs. Anti-MSP1 antibody responses induced by rMSP1-QDs were found to have 2 to 3 log higher titers than those obtained with rMSP1 administered with the conventional adjuvants, Montanide ISA51 and CFA. Moreover, the immune responsiveness and the induction of parasite inhibitory antibodies were significantly superior in mice injected with rMSP1-QDs. The rMSP1-QDs delivered via intra-peritoneal (i.p.), intra-muscular (i.m.), and subcutaneous (s.c.) routes were equally efficacious. The high level of immunogenicity exhibited by the rMSP1-QDs was achieved without further addition of other adjuvant components. Bone marrow derived dendritic cells were shown to efficiently take up the nanoparticles leading to their activation and the expression/secretion of key cytokines, suggesting that this may be a mode of action for the enhanced immunogenicity. This study provides promising results for the use of water soluble, inorganic nanoparticles (<15nm) as potent vehicles/platforms to enhance the immunogenicity of polypeptide antigens in adjuvant-free immunizations.

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Application of Semiconductor Quantum Dots for Breast Cancer Cell Sensing

Cited by 6 publications

References 25 publications

Iron oxide nanoparticles as a clinically acceptable delivery platform for a recombinant blood‐stage human malaria vaccine

Iron oxide nanoparticles as a clinically acceptable delivery platform for a recombinant blood‐stage human malaria vaccine

Quantum Dot Research in Breast Cancer: Challenges and Prospects

Blood stage merozoite surface protein conjugated to nanoparticles induce potent parasite inhibitory antibodies

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