Application of shielding boronate affinity chromatography in the study of the glycation pattern of haemoglobin

Affinity Enrichment and Characterization of Mucin Core-1 Type Glycopeptides from Bovine Serum

“…A recent approach applies porous graphite columns for semiselective enrichment (23), whereas the acidic character of sialylated glycopeptides has also been exploited via titanium dioxide-mediated enrichment (24). Finally vicinal cis-diols can be selectively captured using boronic acid derivatives (25)(26)(27). All methods described here provide some glycopeptide enrichment from non-glycosylated peptide background, but all also suffer from significant non-selective binding.…”

The potential of mass spectrometry to study iron-containing proteins used in clinical diagnosis

“…HbA (␣ 2 ␤ 2 ) is the main type of Hb and represents about 97% of the total Hb. Other minor Hb species are HbA 2 (2.5%) and the fetal Hb (HbF; <1%), where ␤-globins have been replaced by ␦ and ␥-globins, respectively [12].…”

Luminol chemiluminescence biosensor for glycated hemoglobin (HbA1c) in human blood samples

“…A number of analytical methods are currently available to determine HbA1c level (see reference (Pundir and Chawla, 2014) for a review) including immunoassay (Stöllner et al, 2002;Tanaka and Matsunaga, 2001;Qu et al, 2009) ion-exchange chromatography (Eckerbom et al, 1994;Lafferty et al, 2002), boronate affinity chromatography (Li, et al, 2002), electrophoresis (Koval et al, 2011), quartz crystal microbalnce Skládal., 2005, 2006) surface plasmon resonance (Liu et al, 2008), colorometry (Frantzen, et al, 1997), Raman (Barman et al, 2012), and electrochemical methods (Liu et al, 2006;Park et al, 2008;Song and Yoon., 2009;Kim and Shim, 2013). Most of these methods employ chromatographic separations or recognition elements such as HbA1c antibody or boronic acid derivatives before the detection of HbA1c, and then the separated HbA1c is detected with various optical and electrochemical transduction methods.…”