Application of standard addition for the determination of carboxypeptidase activity in Actinomucor elegans bran koji

Fu, Jing-Li; Li, L.; Yang, Xiao-Qun; Zhu, Ming‐Jun

doi:10.1134/s000368381105005x

Cited by 2 publications

(1 citation statement)

References 21 publications

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“…[8,9] In addition, it can be applied as the calibration approach over an external calibration method when matrix effects are present. [10][11][12][13] An interference (constant error) due to a component in the matrix caused by co-elution cannot be corrected by the standard addition method. [4,14] The co-eluant can be evaluated by baseline analysis or testing whether or not the intercept of the external calibration curve is significantly different from zero when an appropriate blank containing real matrix is available.…”

Section: Introductionmentioning

confidence: 99%

Selectivity Investigation and Liquid Chromatographic Method for the Analysis of Nicotine in Tobacco Extracts

Delvadia

Karnes

2013

Journal of Liquid Chromatography & Related Technologies

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& A simple and selective high performance liquid chromatographic (HPLC) method for the analysis of nicotine in a Hanks' balanced salts extract of smokeless tobacco components is presented here. An HPLC method was modified and applied for the analysis of nicotine in samples obtained from a biorelevant in vitro release system for permeation testing of nicotine from a commercially available smokeless tobacco product. The selectivity for the analysis of nicotine in vitro samples was investigated as a part of the method validation by the standard addition method, peak purity testing, and peak trapping followed by mass spectrometry (MS) identification of a co-eluant which was observed. The co-eluting compound was attributable to a leachable substance from the neoprene O-ring used in the apparatus. The mass spectrum (molecular ion with m=z 353.8 % 354) for this substance was consistent with the molecular weight of the plasticizer, alkyl alkylether diester adipate (molecular weight 353), commonly used in manufacturing rubber. Selectivity was shown without chromatographic resolution of the co-eluting substance by selection of an appropriate wavelength range where the co-eluant did not absorb UV light.

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Section: Introductionmentioning

confidence: 99%

Selectivity Investigation and Liquid Chromatographic Method for the Analysis of Nicotine in Tobacco Extracts

Delvadia

Karnes

2013

Journal of Liquid Chromatography & Related Technologies

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A Novel Biorelevant in Vitro System to Predict the in Vivo Performance of Oral Transmucosal Products

Delvadia¹

2013

VCU Theses and Dissertations

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Application of standard addition for the determination of carboxypeptidase activity in Actinomucor elegans bran koji

Cited by 2 publications

References 21 publications

Selectivity Investigation and Liquid Chromatographic Method for the Analysis of Nicotine in Tobacco Extracts

Selectivity Investigation and Liquid Chromatographic Method for the Analysis of Nicotine in Tobacco Extracts

A Novel Biorelevant in Vitro System to Predict the in Vivo Performance of Oral Transmucosal Products

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