Application of step-wise gradient high-performance counter-current chromatography for rapid preparative separation and purification of diterpene components from Pseudolarix kaempferi Gordon

Two-dimensional countercurrent chromatography × high performance liquid chromatography with heart-cutting and stop-and-go techniques for preparative isolation of coumarin derivatives from Peucedanum praeruptorum Dunn

“…The results showed that neither the Arizona system nor the expanded Arizona system could achieve satisfactory Kd values for all of the target compounds (6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16), while the acetone solvent system works well. For example, in solvent systems S1 and S3, the target compounds exhibited much higher Kd values, which were deemed unsuitable for CCC separation.…”

“…Successful applications of CCC have also been reported to separate and purify plant-derived natural products [9]. Recent reports have also revealed that many natural products could be isolated by flow-rate gradient elution CCC or stepwise solvent gradient CCC [10][11][12]. On the other hand, the recently developed high performance countercurrent chromatography (HPCCC), whose separation times are minutes rather than hours, has shown powerful separation ability [13].…”

Suffruticosol A isolated from Paeonia lactiflora seedcases attenuates airway inflammation in mice induced by cigarette smoke and LPS exposure

“…A smaller K value results in coelution near the solvent front with poor resolution, whereas a larger K value tends to give better resolution but broader, more dilute peaks with a longer elution time. The separation factors (α) were lower than 1.5, which was difficult for the HSCCC to separate theoretically (He et al, 2012;Ito, 1992;Zhao, Han, Li, & Yue, 2013;Zhong et al, 2014). To separate the five major compounds in this experiment, the following four solvent systems were tested by UPLC-CAD, and their partition coefficient (K) values are displayed in Table 1.…”

Acid-Alkali Extraction of Triterpene Acids from Poria and Preparative Separation by High-Speed Counter-Current Chromatography

“…The crude samples and each purified fraction were analyzed by HPLC on an Agilent Zorbax SB-C 18 The identification of compounds was carried out by ESI-MS, 1 H NMR, and 13 C NMR spectra.…”

“…In contrast to conventional column chromatography, high-speed countercurrent chromatography (HSCCC) is a liquid-liquid partition chromatography that overcomes the irreversible adsorptive loss of samples onto the solid support (15). This method has been efficiently applied to the separation and purification of different types of natural products (16)(17)(18).…”