Application of T-Pattern Analysis in the Study of Rodent Behavior: Methodological and Experimental Highlights

Casarrubea, Maurizio; Магнуссон, Магнус; Giovanni, Giuseppe Di; Roy, Vincent; Arabo, Arnaud; Santangelo, Andrea; Crescimanno, Giuseppe

doi:10.1007/978-1-4939-3249-8_12

Cited by 4 publications

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“…A more detailed description of theories and concepts concerning T-pattern detection and analysis can be found in our previous articles (Casarrubea et al, 2013, 2016a, 2017a), in two reviews (Casarrubea et al, 2015, 2018) or in our book (Magnusson et al, 2016). On the basis of our previous studies (Casarrubea et al, 2011a,b, 2016b, 2017a,b) the following parameters were analyzed: (1) mean duration (in sec) of behavioral components; (2) mean occurrences of behavioral components; (3) percent distribution of behavioral components; (4) number of different T-patterns detected for each group both in real and random generated data; (5) length distribution of T-patterns; (6) mean length of T-patterns detected; (7) mean occurrences of T-patterns detected and (8) percent distribution of T-patterns including each components of the behavioral repertoire; (9) structure of all the different T-patterns detected for each group (strings).…”

Section: Methodsmentioning

confidence: 99%

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Combining Quantitative and Qualitative Data in the Study of Feeding Behavior in Male Wistar Rats

et al. 2019

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The first step in a behavioral study is represented by the organization of a suitable ethogram, that is, a formal description of individual components of the behavior. Then, each component of such a behavioral repertoire can be quantified (e.g., how many times it occurs, its duration, percent distribution, latency, etc.). However, it is our contention that the possibility to describe the behavior of a living being by means of hundreds or even thousands of numbers concerning isolated components, disjointed from the comprehensive behavioral architecture, does not imply the possibility to use those numbers to reconstruct the meaning of behavior. Such a level of comprehension requires a qualitative approach based on the analysis of behavioral structure and its underlying dynamics. By means of synergic utilization of quantitative and qualitative data a more complete description of a given behavior becomes available. In present study we discuss results obtained from observations of feeding behavior in two groups of male Wistar rats: a control group, under standard diet, and a second group, under hyperglycidic one. Results have been presented both in terms of quantitative evaluations and in terms of structural/qualitative ones, the latter obtained by means of T-pattern detection and analysis. As to quantitative results, mean durations showed a significant reduction of Walking and Feeding and an increase of Hind-Paw Licking and Body Grooming; concerning mean occurrences, a significant increase of Front-Paw Licking, Hind-Paw Licking, and Body Grooming was present; percent distributions showed significant reductions for Walking and Feeding and a significant increase for all grooming activities. As to qualitative assessments, T-pattern analysis unveiled a clear-cut behavioral reorganization induced by the hyperglycidic diet. If on the one hand, 50 different T-patterns were detected in subjects under standard diet, on the other hand, 703 different T-patterns were discovered in animals under hyperglycidic treatment, with a highly significant increase of mean lengths and a significant reduction of mean occurrences of T-patterns. Synergic evaluation of results in terms of quantitative and qualitative aspects shows, in rats fed with hyperglycidic diet, an increased anxiety condition, likely dependent on food-related stimuli and suggestive of a pervasive craving- related behavior.

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Section: Methodsmentioning

confidence: 99%

“…After each observation, OF apparatus was carefully cleaned with ethyl alcohol (70%). Procedural details described in this section were carried out on the basis of our protocols (Casarrubea et al, 2011a,b, 2016b, 2017a,b).…”

Section: Methodsmentioning

confidence: 99%

Combining Quantitative and Qualitative Data in the Study of Feeding Behavior in Male Wistar Rats

et al. 2019

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“…• Preparation of blood films Blood films will be prepared according to the Wedge method described in Houwen, 2002, on days 0, 4, 11, 18, 25, and 32. 74 Then, they will be fixed and stained with Leishman stain and observed under light microscopy for abnormalities in red cell shape, such as fragments (schistocytes, helmet cells, keratocytes), sickle cells, target cells, spherocytes, tear drop cells (dacrocytes) and red cell agglutination. 26,30,75 • Quantitative analysis of serum electrolytes The serum will be used to detect Na⁺, K⁺, Ca²⁺, Fe²⁺, Mg²⁺, Ca²⁺, Zn²⁺, Mn²⁺, Cu²⁺, and Co²⁺ concentrations using an automated method.…”

Section: Animal Studymentioning

confidence: 99%

Anti-anemic properties of Pterocarpus marsupium latex and its cytotoxic and embryotoxic effects.

Basnayake,

Gunatilake,

Goonasekera

et al. 2024

Ind. J. Physiol. Allied Sci.

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Anemia is a hematological disorder, and a large number in the developing world are affected with significant morbidity and mortality. Among them, most depend on traditional medicine to strengthen the hematopoietic action. The availability, low cost, and minor side effects are thought to be associated with this high selection rate of traditional medicine. The Pterocarpus marsupium is a medicinal plant native to Sri Lanka, India, and Nepal. In which the different components are used by traditional medical practitioners in treating different disease conditions, specifically, latex in enhancing hemoglobin production in anemic patients. However, the scientific evidence for using the Pterocarpus marsupium latex as an anti-anemic agent is minimal. Therefore, this study is planned to evaluate its anti-anemic effect, erythropoietin (EPO)-like effect, toxicity, phytochemical composition, and bioactive ingredients. Consequently, we aim to apply in vitro and in vivo assays to investigate the toxicity and favorable anti-anemic and other hematopoietic potentials of the plant latex using normal human cell cultures, rat-derived hematopoietic stem cell cultures, zebrafish embryos, and phenyl hydrazine-induced anemic rats with identification of bioactive ingredients that might mediate such potentials. If we discover a similar action of EPO in this plant latex, it would be beneficial in the way of treating anemia-associated disease conditions such as chronic renal failure, myelodysplasia, rheumatoid arthritis, HIV, and cancer as a replacement for recombinant human EPO (rhEPO). This will be beneficial in the way of reducing the high cost of rhEPO treatment as well. Finally, an attempt to discover a therapeutic agent for anemia from a safe, natural source will considerably contribute to traditional medicine/herbalism due to the crucial role of traditional medicine in the prophylaxis or therapy of hematological diseases in Sri Lanka. Keywords: Pterocarpus marsupium; Indian kino; Anemia; Toxicity; Latex; Erythropoietin

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“…The first was the T-pattern concept, a statistical hierarchical self-similar (pseudo-fractal) pattern recurring with significant translation symmetry since detected in human, animal, and brain network behavior (Magnusson, 1996 , 2000 , 2005 ; Casarrubea et al, 2015 , 2018 ; Magnusson et al, 2016 ; Casarrubea and Di Giovanni, 2020 ). Gradually, more structural concepts i.e., T-meme, T-composition, T-associate, T-packet, T-music, T-string, T-religion, T-money, and T-society have been added constituting the T-system (Magnusson, 2020 ).…”

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confidence: 99%

Editorial: Behavior and self-similarity between nano and human scales: from T-pattern and T-string analysis (TPA) with THEME to T-societies

et al. 2023

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Application of T-Pattern Analysis in the Study of Rodent Behavior: Methodological and Experimental Highlights

Cited by 4 publications

References 44 publications

Combining Quantitative and Qualitative Data in the Study of Feeding Behavior in Male Wistar Rats

Combining Quantitative and Qualitative Data in the Study of Feeding Behavior in Male Wistar Rats

Anti-anemic properties of Pterocarpus marsupium latex and its cytotoxic and embryotoxic effects.

Editorial: Behavior and self-similarity between nano and human scales: from T-pattern and T-string analysis (TPA) with THEME to T-societies

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