“…Metronidazole (M), secnidazole (S), ornidazole (O), and tinidazole (T) are drugs commonly used to treat protozoan infestations and infections caused by anaerobic bacteria. The scientific literature describes using thin-layer chromatography methods for the determination of selected 5-nitoimidazole in the presence of another drug, e.g., metronidazole and di-iodohydroxychinoline [16], diiodohydroxyquine [17], spiramycin [18], furazolidone [19,20], loperamide [19], tetracycline Processes 2024, 12, 643 2 of 17 hydrochloride [21], ciprofloxacin [22], diloxamide furoate [23], and clotrimazole [24] as well as tinidazole and clotrimazole [25,26], omeprazole [27], clarithromycin [27], fluconazole [28,29], norfloxacin [30][31][32], and ciprofloxacin [33]. TLC methods presented in the scientific literature are often not fully validated.…”