Application of the micronucleus test and comet assay in Trachemys callirostris erythrocytes as a model for in situ genotoxic monitoring

García, Lina María; Bock, Brian C.; Orozco, Luz Yaneth; Palacio, Jaime

doi:10.1016/j.ecoenv.2016.01.016

Cited by 42 publications

(18 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The statistical analysis was performed by the Z test which compares two Poisson curves. The result was considered positive if a significant difference was observed in relation to the negative control, at least P < 0.05 (Zar, 1996).…”

Section: Micronucleus Test In V79 Cell Linementioning

confidence: 99%

See 1 more Smart Citation

Genotoxicity biomarkers for airborne particulate matter (PM2.5) in an area under petrochemical influence

Lemos

Flores

et al. 2016

Chemosphere

View full text Add to dashboard Cite

Section: Micronucleus Test In V79 Cell Linementioning

confidence: 99%

“…Therefore, biomarkers that are sensitive to detect toxic substances in genetic material are important tools to adopt measures to prevent damage caused by environmental pollution (Park and Choi, 2007;Van der Oost et al, 2003;Zapata et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Genotoxicity biomarkers for airborne particulate matter (PM2.5) in an area under petrochemical influence

Lemos

Flores

et al. 2016

Chemosphere

View full text Add to dashboard Cite

“…We found intra-specific variation in DNA damage levels and DNA repair capacity in both species (T. carolina and T. scripta). Intra-specific variation in DNA damage is commonly used in biomonitoring studies to compare samples from sites experiencing different levels of contamination (Bonisoli-Alquati et al, 2010;Zapata et al, 2016) or different stress levels (Robert and Bronikowski, 2010). In this study, individuals were all sampled at the same site and during the same season and therefore the detected differences in levels of DNA damage among individuals are most likely not due to contamination, stress, or seasonal variation among samples; see (Jha, 2008) for potential sources of variation.…”

Section: Discussionmentioning

confidence: 99%

Application of the CometChip platform to assess DNA damage in field‐collected blood samples from turtles

Sýkora

Chiari

Heaton

et al. 2018

Environ and Mol Mutagen

View full text Add to dashboard Cite

DNA damage has been linked to genomic instability and the progressive breakdown of cellular and organismal homeostasis, leading to the onset of disease and reduced longevity. Insults to DNA from endogenous sources include base deamination, base hydrolysis, base alkylation, and metabolism-induced oxidative damage that can lead to single-strand and double-strand DNA breaks. Alternatively, exposure to environmental pollutants, radiation or ultra-violet light, can also contribute to exogenously derived DNA damage. We previously validated a novel, high through-put approach to measure levels of DNA damage in cultured mammalian cells. This new CometChip Platform builds on the classical single cell gel electrophoresis or comet methodology used extensively in environmental toxicology and molecular biology. We asked whether the CometChip Platform could be used to measure DNA damage in samples derived from environmental field studies. To this end, we determined that nucleated erythrocytes from multiple species of turtle could be successfully evaluated in the CometChip Platform to quantify levels of DNA damage. In total, we compared levels of DNA damage in 40 animals from two species: the box turtle (Terrapene carolina) and the red-eared slider (Trachemys scripta elegans). Endogenous levels of DNA damage were identical between the two species, yet we did discover some sex-linked differences and changes in DNA damage accumulation. Based on these results, we confirm that the CometChip Platform allows for the measurement of DNA damage in a large number of samples quickly and accurately, and is particularly adaptable to environmental studies using field-collected samples. Environ. Mol. Mutagen. 59:322-333, 2018. © 2018 Wiley Periodicals, Inc.

show abstract

“…callirostris (turtle species endemic) in order to know the levels of DNA damage in this species and demonstrated its possible use for screening genotoxic effects in freshwater environments. Results showed that the frequency of micronuclei in the samples was positively related to comet tail length and tail moment and that both genotoxicity biomarkers may be applied to T. callirostris erythrocytes as a species of choice for the evaluation of environmental pollutants effects in freshwater [28]. Mellado-Garcia, Puerto et al, used Comet assay in liver and stomach, and Micronucleus Test in bone marrow to assess the genotoxicity of propyl thiosulphinate oxide (PTSO); principal constituent of Allium tobacco [33].…”

Section: Recents Studies Using Comest Assaymentioning

confidence: 99%

Comet Assay on Toxicogenetics; Several Studies in Recent Years on Several Genotoxicological Agents

Gharsalli¹

2016

J Environ Anal Toxicol

View full text Add to dashboard Cite

Application of the micronucleus test and comet assay in Trachemys callirostris erythrocytes as a model for in situ genotoxic monitoring

Cited by 42 publications

References 75 publications

Genotoxicity biomarkers for airborne particulate matter (PM2.5) in an area under petrochemical influence

Genotoxicity biomarkers for airborne particulate matter (PM2.5) in an area under petrochemical influence

Application of the CometChip platform to assess DNA damage in field‐collected blood samples from turtles

Comet Assay on Toxicogenetics; Several Studies in Recent Years on Several Genotoxicological Agents

Contact Info

Product

Resources

About