Application of the Response Surface Methodology to Optimize the Fermentation Parameters for Enhanced Docosahexaenoic Acid (DHA) Production by Thraustochytrium sp. ATCC 26185

Wu, Kang; Ding, Lijian; Zhu, Peng; Li, Shuang; He, Shan

doi:10.3390/molecules23040974

Cited by 22 publications

(7 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thraustochytrium sp. ATCC 26185 previously showed enhanced DHA production, from 1.16 to 1.68 g/L, after optimization of various fermentation parameters, such as initial pH (6.89), inoculum volume (4.16%), and fermentation volume (140.47 mL) [50]. Moreover, cultivation of thraustochytrid strains in flasks resulted in significantly different levels of DHA production compared to bioreactor cultivation.…”

Section: Resultsmentioning

confidence: 99%

Simultaneous production of DHA and squalene from Aurantiochytrium sp. grown on forest biomass hydrolysates

Patel

Rova

Christakopoulos

et al. 2019

Biotechnol Biofuels

View full text Add to dashboard Cite

BackgroundRecent evidence points to the nutritional importance of docosahexaenoic acid (DHA) in the human diet. Thraustochytrids are heterotrophic marine oleaginous microorganisms capable of synthesizing high amounts of DHA, as well as other nutraceutical compounds such as squalene, in their cellular compartment. Squalene is a natural triterpene and an important biosynthetic precursor to all human steroids. It has a wide range of applications in the cosmetic and pharmaceutical industries, with benefits that include boosting immunity and antioxidant activity. Apart from its nutritional quality, it can also be utilized for high-grade bio-jet fuel by catalytic conversion.ResultsIn the present study, the potential of thraustochytrid strain Aurantiochytrium sp. T66 to produce DHA and squalene was evaluated. When the strain was cultivated on organosolv-pretreated birch hydrolysate (30 g/L glucose) in flask, it resulted in 10.39 g/L of cell dry weight and 4.98 g/L of total lipids, of which 25.98% was DHA. In contrast, when the strain was grown in a bioreactor, cell dry weight, total lipid, and DHA increased to 11.24 g/L, 5.90 g/L, and 35.76%, respectively. The maximum squalene yield was 69.31 mg/gCDW (0.72 g/L) when the strain was cultivated in flask, but it increased to 88.47 mg/gCDW (1.0 g/L), when cultivation shifted to a bioreactor.ConclusionsThis is the first report demonstrating the utilization of low cost non-edible lignocellulosic feedstock to cultivate the marine oleaginous microorganism Aurantiochytrium sp. for the production of nutraceutical vital compounds. Owing to the simultaneous generation of DHA and squalene, the strain is suitable for industrial-scale production of nutraceuticals.

show abstract

Section: Resultsmentioning

confidence: 99%

Simultaneous production of DHA and squalene from Aurantiochytrium sp. grown on forest biomass hydrolysates

Patel

Rova

Christakopoulos

et al. 2019

Biotechnol Biofuels

View full text Add to dashboard Cite

show abstract

“…Therefore, in order to address this issue, the RSM was deployed. The RSM is a useful statistical optimization design that has successfully been used in many biological and chemical processes [34][35][36]. The RSM creates an experimental design with the minimum number of experiments and generates a model that can predict the interaction and correlation between a set of independent variables and observed results, thus providing optimized conditions that differ from the conventional approach of optimization, which only investigates one factor at a time.…”

Section: Screening Of Phytohormones For Improved Growth and Dha Production Of Sw1mentioning

confidence: 99%

Different Classes of Phytohormones Act Synergistically to Enhance the Growth, Lipid and DHA Biosynthetic Capacity of Aurantiochytrium sp. SW1

et al. 2020

View full text Add to dashboard Cite

In the present study, the impact of eight phytohormones from six different classes on the growth, lipid and docosahexaenoic acid (DHA) biosynthetic capacity of Aurantiochytrium sp. SW1 (SW1) was evaluated. Kinetin (KIN), jasmonic acid (JA) and gibberellic acid (GA) significantly enhanced the growth and DHA production of SW1 by 16%–28% and 66%–84% in comparison to the control, respectively. The synergistic effect of these three phytohormones, evaluated by the response surface methodology (RSM), showed that a combination of 3.6 mg/L GA, 2.0 mg/L KIN and 20.0 mg/L JA further increased the growth and DHA production of SW1 by 16% to 28% and 22% to 36%, respectively, in comparison to the individual supplementation. The synergistic effect of these phytohormones was also shown to be time-dependent, where feeding at 24 h of cultivation led to 15%, 26% and 35% further increments in the biomass, lipid and DHA production in comparison to that of 0 h, respectively. The determination of stress markers, antioxidant enzymes and key enzymes involved in fatty acid biosynthesis aided to elucidate the potential mechanism underlying the improvement of growth and DHA production by SW1 at various times of feeding. Supplementation with the phytohormones at 24 h exhibited the maximum impact on reducing the level of reactive oxygen species (ROS) and malondialdehyde (MDA), as well as augmented the antioxidants (superoxide dismutase and catalase) and key metabolic enzymes involved in lipogenesis (malic, glucose-6-phosphate dehydrogenase and ATP-citrate lyase) in comparison to the control and other time points. This study signifies the potential application of phytohormones for improving the growth, lipid and DHA production in Aurantiochytrium spp.

show abstract

“…Box-Behnken design. The response surface methodology is a very practical and valuable tool for fermentation industry to improve product yield and minimize by‐products as well as reduce overall manufacturing costs [15], and it is possible for response surface methodology to derive an expression for performance measurement on the basis of the response values obtained from experiments at a particular combination of input variables [16]. In the present study, by employing Box-Behnken design and response surface methodology, the effects of the three independent variables (loaded liquid, 15-60 mL/250mL; liquid pH, 7.0-8.0; culture temperature, 24-32°C) and three levels (high, middle, and low) on the response (the total number of living bacteria) were investigated to determine the optimal conditions, which maximized the biomass of B. megaterium from shake cultivation.…”

Section: Methodsmentioning

confidence: 99%

Optimization of Cultural Conditions for Bacillus megaterium Cultured in Agaricus bisporus Industrial Wastewater

Huang

Zhang

et al. 2018

BioMed Research International

View full text Add to dashboard Cite

The aim of this study was to optimize the cultural conditions for Bacillus megaterium using Agaricus bisporus industrial wastewater as nature culture through response surface methodology. In our present study, we analyzed the total number of living B. megaterium in the fermentation broth using multispectral imaging flow cytometry. Plackett-Burman design was applied to evaluate the effects of six variables, namely, initial pH, industrial wastewater solubility, rotating speed, culture temperature, inoculum size, and loading volume. Loading volume, initial pH, and culture temperature were found to influence the biomass of B. megaterium significantly and were further optimized by Box-Behnken design. After verification test, the optimum fermentation conditions of B. megaterium using the A. bisporus processing wastewater as nature culture media were obtained as follows: initial pH of 7.4, culture temperature of 25°C, loading volume of 40 mL/250 mL, culture time of 24 h, industrial wastewater solubility of 1%, rotating speed of 200 rpm, and inoculum size of 8%. The predicted optimum model's value was 8.88 × 108 Obj/mL and the average experimental value was 9.03 ± 0.02 × 108 Obj/mL, which met the national microbial fertilizers' standard. Furthermore, the field experiment results showed that the fermentation broth of B. megaterium could significantly improve the yield of Spinacia oleracea L.

show abstract

Application of the Response Surface Methodology to Optimize the Fermentation Parameters for Enhanced Docosahexaenoic Acid (DHA) Production by Thraustochytrium sp. ATCC 26185

Cited by 22 publications

References 34 publications

Simultaneous production of DHA and squalene from Aurantiochytrium sp. grown on forest biomass hydrolysates

Simultaneous production of DHA and squalene from Aurantiochytrium sp. grown on forest biomass hydrolysates

Different Classes of Phytohormones Act Synergistically to Enhance the Growth, Lipid and DHA Biosynthetic Capacity of Aurantiochytrium sp. SW1

Optimization of Cultural Conditions for Bacillus megaterium Cultured in Agaricus bisporus Industrial Wastewater

Contact Info

Product

Resources

About