Application of thin layer chromatography for the analytical diagnostics of combined prochlorperazine poisonings

Quetiapine fumarate (an antipsychotic) is part of numerous generic drugs that are in fairly wide demand among the population, therefore, more and more data appear on the counterfeiting and smuggling of funds, as well as non-medical use, which are life-threatening for the population and explain the high prevalence of the active ingredient as object of forensic examination. The aim. To develop an algorithm for conducting a forensic pharmaceutical examination and propose a method for determining quetiapine fumarate for forensic pharmaceutical purposes. Materials and methods. All studies were performed using reagents that meet the EP, USP and USPU requirements, Class A glassware and qualified devices. Identification by IR spectroscopy was performed in the range from 500 to 4000 cm-1 on the device “Nicolet 380 FT-IR Spectrometer by Thermo Fisher Scientific” using a prefix “Smart Perfomer” with a ZnSe crystal. The UV absorption spectra of the solutions were recorded using a Specord 205 spectrophotometer from Analytik Jena AG (Germany). TLC was performed on Merck chromatographic plates (silica gel 60G F254, Germany). The following systems were used as mobile phases: hexane – acetone – 25 % ammonia solution (60: 40: 2); methanol – 25 % ammonia solution (100: 1.5), hexane – acetone – 25 % ammonia solution (50: 45: 5). Detection was performed under UV light (254 nm), followed by spraying with Dragendorff reagent. Analysis by gas chromatography with mass detection was performed using a GC gas chromatograph with a mass spectrometric detector GCMS-QP2020. Data were analyzed using the program: GCMSsolution, LabSolutions Insight (Shimadzu Corporation, Tokyo, Japan). Results. An algorithm for conducting a forensic pharmaceutical examination in accordance with the current legislation of Ukraine has been developed, methods for determining quetiapine for forensic pharmaceutical purposes have been proposed. Conclusions. The developed methods for determining quetiapine meet the requirements of the current legislation of Ukraine and the Ministry of Justice of Ukraine. The obtained data prove the high sensitivity and reproducibility of the methods and prove the possibility of their introduction into the practice of forensic examination

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A Difference Spectrophotometric Method for Determination of Alimemazine Based on the Absorbance of its Sulphoxide

Blazheyevskiy,

Kryskiw,

Kucher

et al. 2023

MOCA

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A new difference spectrophotometric method for the analysis of alimemazine in commercial formulations has been proposed. The method is based on oxidizing the drug with potassium caroate for the formation of corresponding sulfoxide (ε = (4.7 ± 0.06) · 103 L mol-1 cm-1) and measuring the absorbance of the solution at 342 nm using an unoxidized drug of the same concentration as blank. The graph of Beer's law for alimemazine tartrate showed that the ΔA values measured at the corresponding wavelength of maximum absorbance difference are directly proportional to the drug concentration in oxidized solutions in the range 2-30 μg/mL. The characteristics of the calibration curve were A = (b ± Δb) · C - (a ± Δa) = (0.0539 ± 0.0014) · C - 0.017 ± 0.026; LOD = 0.68 μg mL-1; LOQ = 2.07 μg mL-1. The proposed method was successfully applied for the analysis of alimemazine-containing commercial pharmaceutical formulations: Teraligen Valenta tablets 5.0 mg and Theralene 4 %, oral solution. RSD ≤ 1.8 % (δ= – 0.82 % and –1.30 % respectively).

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Application of thin layer chromatography for the analytical diagnostics of combined prochlorperazine poisonings

Cited by 3 publications

References 5 publications

Rational design of manganese oxide/tin oxide hybrid nanocomposite based electrochemical sensor for detection of prochlorperazine (Antipsychotic drug)

Rational design of manganese oxide/tin oxide hybrid nanocomposite based electrochemical sensor for detection of prochlorperazine (Antipsychotic drug)

Development of determination methods of quetiapine fumarate for forensic-pharmaceutical purposes

A Difference Spectrophotometric Method for Determination of Alimemazine Based on the Absorbance of its Sulphoxide

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