Applications of flow cytometry sorting in the pharmaceutical industry: A review

Abstract

The article reviews applications of flow cytometry sorting in manufacturing of pharmaceuticals. Flow cytometry sorting is an extremely powerful tool for monitoring, screening and separating single cells based on any property that can be measured by flow cytometry. Different applications of flow cytometry sorting are classified into groups and discussed in separate sections as follows: (a) isolation of cell types, (b) high throughput screening, (c) cell surface display, (d) droplet fluorescent-activated cell sorting (FACS). Future opportunities are identified including: (a) sorting of particular fractions of the cell population based on a property of interest for generating inoculum that will result in improved outcomes of cell cultures and (b) the use of population balance models in combination with FACS to design and optimize cell cultures.

Function-driven design of Bacillus kochii and Filobasidium magnum co-culture to improve quality of flue-cured tobacco

“…In our study, flow cytometry is a powerful method for screening and isolating single cells through high-throughput equipment compared to traditional plate isolation methods ( Vitelli et al, 2021 ). Via flow cytometry, the obtained library of tobacco microbes can provide a screening source for functional microbial.…”

Bioaugmentation of Bacillus amyloliquefaciens-Bacillus kochii co-cultivation to improve sensory qualities of flue-cured tobacco

“…Flow cytometry sorting is a powerful tool for monitoring, screening and separating single cells (Vitelli et al 2021). Compared with traditional cultural methods, ow cytometry can isolate the individual cells with high precision, high throughput and low cost (Zhang et al, 2018).…”

Sampling methods for outdoor sculptures: Comparison of swabs and cryogels by flow cytometry as novel alternatives for assessment and quantification of microbial contamination

“…The use of rapid and well-established techniques, such as flow cytometry, can be a valuable and effective tool not only to quantify the microbial communities present in the artworks but also to infer their viability and metabolic state using appropriate fluorochromes. Flow cytometry has been successfully used over the years in several fields of research, for the analysis of environmental, pharmaceutical, medical and food samples [17,18,27,28,[19][20][21][22][23][24][25][26]. In the context of CH, however, its application is still limited, although some studies have used flow cytometry as a complement to other analytical tools, either in the study of the microorganisms involved in the biodeterioration processes of artworks or the evaluation of the efficiency of potential biocide treatments [29][30][31][32][33][34].…”