Applications of high‐resolution magic angle spinning MRS in biomedical studies II—Human diseases

Abstract

High-resolution magic angle spinning (HRMAS) magnetic resonance spectroscopy (MRS) is a powerful method for gaining insight into the physiological and pathological processes of cellular metabolism. Given its ability to obtain high resolution spectra of non-liquid biological samples, while preserving tissue architecture for subsequent histopathological analysis, the technique has become invaluable for biochemical and biomedical studies. Using HRMAS MRS, alterations in measured metabolites, metabolic ratios, and metabolomic profiles present the possibility to improve identification and prognostication of various diseases and decipher the metabolomic impact of drug therapies. In this review, we evaluate HRMAS MRS results on human tissue specimens from malignancies and non-localized diseases reported in the literature since the inception of the technique in 1996. We present the diverse applications of the technique in understanding pathological processes of different anatomical origins, correlations with in vivo imaging, effectiveness of therapies, and progress in the HRMAS methodology.

Metabolic profiling of listeria rhombencephalitis in small ruminants by 1H high‐resolution magic angle spinning NMR spectroscopy

“…HR‐MAS NMR has provided insights into the metabolic basis of disease and the identification of biomarkers for neoplastic and degenerative CNS diseases and various other brain dysfunctions . Recently, two excellent reviews have been published reviewing the applications of HR‐MAS magnetic resonance spectroscopy (MRS) in biomedical studies . However, there have only been a few reports on infectious diseases of the CNS .…”

Inherently decoupled1H antennas and31P loops for metabolic imaging of liver metastasis at 7 T

“…8 In the preclinical setting, 31 P spectroscopy is already a key tool for elucidating cancer metabolism and quantitative evaluation of new treatments. 9 As such, clinical 31 P spectroscopy is of great interest as a potential tool for tracking treatment response. 8 10 These may all be related to early treatment response.…”

Cancer metabolism in a snapshot: MRS(I)

“…On the other hand, single voxel (SV) MRS still seems to play a role in brain and breast cancer, but not so much for prostate cancer. Ex vivo HRMAS studies in the period investigated in this review seem to be mostly used in breast and prostate cancer, and there is a recent review on the matter that analyses its use in detail …”