Applications of polydopamine modifications in capillary electrophoretic analysis

Abstract

Mussel-inspired polydopamine has been widely used in capillary electrophoresis as a facile and universal tool for the surface modification of capillary, mainly due to its versatility, stability, strong adhesiveness, and biocompatibility properties. In this review, the recent development of mussel-inspired surface chemistry with rapid deposition of polydopamine was introduced, and the recent applications of polydopamine in capillary electrophoresis (2011-July 2018) were summarized into four main aspects, namely, sample pretreatments, functional coatings, codeposition coatings, and intermediate coatings. Further study may be focused on clarifying the mechanisms of polydopamine formation and polydopamine-assisted codeposition, and expanding coatings methods to plant polyphenols.

Recent developments in capillary and microchip electroseparations of peptides (2019–mid 2021)

“…Latest developments in column coatings for CE of proteins applicable also to peptides are presented in the earlier reviews [91, 92]. A recent review deals with the application of selected polydopamine modifications of FS capillaries in CE [93].…”

Post translational modification-assisted cancer immunotherapy for effective breast cancer treatment

“…21 Briey, DMSN were prepared rst 23 and then a thin polydopamine (PDA) coating (named PDA-DMSN) was applied. PDA 24 was chosen for its biocompatibility and chelating ability with metal ions. Finally, titanium modication was performed by treating PDA-DMSN with titanium tetrachloride to obtain the nal TiDMSN product.…”

Sulfonic Functionalized Polydopamine Coatings with pH-Independent Surface Charge for Optimizing Capillary Electrophoretic Separations

“…Benefiting from the strong substrate-independent surface adhesive properties, versatile reactivity, relatively simple fabrication processes similar to physically adsorbed coatings, and favorable stability as covalent coatings, mussel-inspired polydopamine (PDA) and its derivative materials are highly suitable for various surface modification applications [16][17][18], including the inner wall modification of fused-silica capillaries [19]. Several functional PDA coated columns have been developed for CE or capillary electrochromatography (CEC) separations in different application fields [20,21], including the separation of small organic molecules [22][23][24][25], protein separation [26,27], chiral separation [28][29][30][31], etc. However, most existing PDA-based capillary modification strategies in CE, involving alkaline pH-induced oxidative polymerization of dopamine, often exhibit a poor surface masking effect and require an extended time ranging from over 20 h to several days, thus limiting reproducibility and practicality.…”