Approaches to Biomaterials Testing According to Modern Biocompatibility Paradigm

Викнянщук, А. Н.; Mishanin, Alexander I.; Tverdokhlebov, Sergei I.; Головкин, А. С.

doi:10.18705/2311-4495-2017-4-1-29-40

Cited by 3 publications

(1 citation statement)

References 23 publications

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“…The data obtained were analyzed using the Kaluza 2.1 software (Beckman Coulter). Double positive events were identified as cells in late apoptosis or necrosis state; Annexin V positive corresponded to cells in early apoptosis; and double negative to viable cells [20][21][22].…”

Section: Cytotoxic Properties Of Agnpsmentioning

confidence: 99%

Hemolytic Activity, Cytotoxicity, and Antimicrobial Effects of Human Albumin- and Polysorbate-80-Coated Silver Nanoparticles

et al. 2021

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In this study, we aimed to develop a technique for colloidal silver nanoparticle (AgNP) modification in order to increase their stability in aqueous suspensions. For this purpose, 40-nm spherical AgNPs were modified by the addition of either human albumin or Tween-80 (Polysorbate-80). After detailed characterization of their physicochemical properties, the hemolytic activity of the nonmodified and modified AgNPs was investigated, as well as their cytotoxicity and antimicrobial effects. Both albumin- and Tween-80-coated AgNPs demonstrated excellent stability in 0.9% sodium chloride solution (>12 months) compared to nonmodified AgNPs, characterized by their rapid precipitation. Hemolytic activity of nonmodified and albumin-coated AgNPs was found to be minimal, while Tween-80-modified AgNPs produced significant hemolysis after 1, 2, and 24 h of incubation. In addition, both native and Tween-80-covered AgNPs showed dose-dependent cytotoxic effects on human adipose-tissue-derived mesenchymal stem cells. The albumin-coated AgNPs showed minimal cytotoxicity. The antimicrobial effects of native and albumin-coated AgNPs against S. aureus, K. pneumonia, P. aeruginosa, Corynebacterium spp., and Acinetobacter spp. were statistically significant. We conclude that albumin coating of AgNPs significantly contributes to improve stability, reduce cytotoxicity, and confers potent antimicrobial action.

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Section: Cytotoxic Properties Of Agnpsmentioning

confidence: 99%

Hemolytic Activity, Cytotoxicity, and Antimicrobial Effects of Human Albumin- and Polysorbate-80-Coated Silver Nanoparticles

et al. 2021

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Biocompatibility of electrospinning polycaprolactone, polylactic acid, their blends and copolymers scaffolds in in vitro tests if mesenchyme stem cells

Mishanin

Panina

Bolbasov

et al. 2021

Transl. med. (Print)

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Background. Biodegradable polymers are one of the most promising groups of materials suitable for creating tissue-engineered scaffolds. The high interest in biopolymers is associated with the possibility of creating scaffolds with desired properties, through the use of mixtures and copolymers. The determination of the key parameters of biocompatibility is the basic purpose for testing created materials.Objective. To perform the comparative in vitro study of biocompatibility properties of biopolymer scaffolds produced using polycaprolactone, polylactic acid, their mixtures and copolymers by electrospinning technology.Design and methods. The adhesion properties and cytotoxicity of scaffolds made from polycaprolactone, polylactic acid, copolymer of L- and D-isoforms of lactic acid, their mixtures and co-polymers with the addition of polyglycolic acid were investigated after scaffolds co-cultivation with human mesenchyme stem cells (MSC).Results. The largest number of spread spindle-shaped MSCs was on the surface of polymers containing polyglycolic acid. Besides, the cells on the surface of the copolymer with polyglycolic acid had the morphology closest to the control. The lowest number of living cells was found on the surface of polylactic acid scaffolds, and the highest on the surface of samples from of polycaprolactone and polylactic acid blend.Conclusion. Thus, all tested polymers had good adhesion properties in experiments with human mesenchyme stem cells were possessed by biodegradable polymers with the addition of polyglycolic acid.

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Current state and development prospects of fundamental studies in research institutions of the National Academy of Medical Sciences of Ukraine

Цымбалюк¹,

Medvedovska²

2019

Journal NAMSU

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Fundamental scientific studies have always been and will always be a priority in Ukrainian academic medical science. Particular attention must be paid to the development of innovations and scientific and technical develop- ments in the medical industry. The National Academy of Medical Sciences of Ukraine endorses basic principles of further progressive development of medical science in the field of prevention of diseases, their early diagnosis, treatment and effective methods of rehabilitation. The Academy designates the strategic directions of research and innovation, ways to implement tasks and activities of a medical, economic, legal and social pattern, with the aim of maintaining and strengthening human health, as well as extending active longevity of the population. The implementation of the main directions of modern fundamental research at the level of the leading global institutions calls for a significant improvement in methodology, technologies and scien- tific workforce on par with the leading scientific institutions worldwide. This will give a new impetus to the development, implementation and continuity of fundamental research in medical science considering demographic, socio-hygienic and socio-economic factors that have a formative role for the health of the nation. The organization of research and production enterpris- es within the framework of academic institutions with the elimination of interdepartmental barriers, a more complex scientific research, the rational use of the funding, and the active implementation of results in health care practice is also of great importance to the further development of the funda- mental research of the medical science in Ukraine. The development of the global medical science is continuous, while priorities and key development points show the tendency to sway toward biomedical research and biotech- nology, genetic engineering and personalized high-tech medicine. Key words: fundamental studies, NAMS of Ukraine, medical science, innovation, health care.

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Approaches to Biomaterials Testing According to Modern Biocompatibility Paradigm

Cited by 3 publications

References 23 publications

Hemolytic Activity, Cytotoxicity, and Antimicrobial Effects of Human Albumin- and Polysorbate-80-Coated Silver Nanoparticles

Hemolytic Activity, Cytotoxicity, and Antimicrobial Effects of Human Albumin- and Polysorbate-80-Coated Silver Nanoparticles

Biocompatibility of electrospinning polycaprolactone, polylactic acid, their blends and copolymers scaffolds in in vitro tests if mesenchyme stem cells

Current state and development prospects of fundamental studies in research institutions of the National Academy of Medical Sciences of Ukraine

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