Appropriate Animal Models for Translational Nerve Research

Haastert‐Talini, Kirsten

doi:10.1007/978-3-030-06217-0_9-1

Cited by 7 publications

(11 citation statements)

References 79 publications

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“…Overall, we must conclude from the studies discussed above that more thorough analysis of differentiated SCs and comprehensive characterization of their properties in vivo [ 94 ] will be needed prior to any translation into clinical use in cell therapies.…”

Section: Characterizing Schwann Cells Differentiated From Ipscsmentioning

confidence: 99%

Perspective on Schwann Cells Derived from Induced Pluripotent Stem Cells in Peripheral Nerve Tissue Engineering

Huang

Powell

Phillips

et al. 2020

Cells

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Schwann cells play a crucial role in successful peripheral nerve repair and regeneration by supporting both axonal growth and myelination. Schwann cells are therefore a feasible option for cell therapy treatment of peripheral nerve injury. However, sourcing human Schwann cells at quantities required for development beyond research is challenging. Due to their availability, rapid in vitro expansion, survival, and integration within the host tissue, stem cells have attracted considerable attention as candidate cell therapies. Among them, induced pluripotent stem cells (iPSCs) with the associated prospects for personalized treatment are a promising therapy to take the leap from bench to bedside. In this critical review, we firstly focus on the current knowledge of the Schwann cell phenotype in regard to peripheral nerve injury, including crosstalk with the immune system during peripheral nerve regeneration. Then, we review iPSC to Schwann cell derivation protocols and the results from recent in vitro and in vivo studies. We finally conclude with some prospects for the use of iPSCs in clinical settings.

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Section: Characterizing Schwann Cells Differentiated From Ipscsmentioning

confidence: 99%

Perspective on Schwann Cells Derived from Induced Pluripotent Stem Cells in Peripheral Nerve Tissue Engineering

Huang

Powell

Phillips

et al. 2020

Cells

Self Cite

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“…However, it must be noted that the fast speed of nerve regeneration following axonotmesis also has disadvantageous implications. Due to the fast speed of nerve regeneration, and, therefore, functional recovery in rodents, differences in nerve regeneration which might relate to the respective experimental treatment approaches or therapies are difficult to detect, hampering translational research in this small animal models [ 26 , 39 ].…”

Section: Discussionmentioning

confidence: 99%

“…In regard to the sciatic nerve model of the rat, a comprehensive systematic review published in 2021 has summarized the functional outcome following nerve injury and repair in this commonly used model of peripheral nerve injury [ 1 ]. Several authors have addressed the advantages and disadvantages of the rat sciatic nerve model [ 6 , 25 , 26 ]. A comprehensive review and meta-analysis of the course of functional recovery can serve as a valuable aid to researchers aiming to test a new therapeutic approach or reconstructive technique in this model when conceptualizing and planning their study.…”

Section: Introductionmentioning

confidence: 99%

The Grasping Test Revisited: A Systematic Review of Functional Recovery in Rat Models of Median Nerve Injury

et al. 2022

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The rat median nerve model is a well-established and frequently used model for peripheral nerve injury and repair. The grasping test is the gold-standard to evaluate functional recovery in this model. However, no comprehensive review exists to summarize the course of functional recovery in regard to the lesion type. According to PRISMA-guidelines, research was performed, including the databases PubMed and Web of Science. Groups were: (1) crush injury, (2) transection with end-to-end or with (3) end-to-side coaptation and (4) isogenic or acellular allogenic grafting. Total and respective number, as well as rat strain, type of nerve defect, length of isogenic or acellular allogenic allografts, time at first signs of motor recovery (FSR) and maximal recovery grasping strength (MRGS), were evaluated. In total, 47 articles met the inclusion criteria. Group I showed earliest signs of motor recovery. Slow recovery was observable in group III and in graft length above 25 mm. Isografts recovered faster compared to other grafts. The onset and course of recovery is heavily dependent from the type of nerve injury. The grasping test should be used complementary in addition to other volitional and non-volitional tests. Repetitive examinations should be planned carefully to optimize assessment of valid and reliable data.

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“…Application of ESWT to improve nerve regeneration was first described by Hausner et al in a rat model of sciatic nerve autograft repair [ 27 ]. The sciatic nerve injury model, especially in case of neurotmesis injuries, has several drawbacks mostly related to compromised animal welfare due to the onset of neuropathic pain, joint contractures and automutilation [ 28 , 29 ]. As the sciatic nerve supplies innervation both to flexor and extensor muscles of the hind paw, misdirection of axons can easily occur following neurotmesis injuries in which by definition the fascicular structure is lost.…”

Section: Introductionmentioning

confidence: 99%

ESWT Diminishes Axonal Regeneration following Repair of the Rat Median Nerve with Muscle-In-Vein Conduits but Not after Autologous Nerve Grafting

et al. 2022

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Investigations reporting positive effects of extracorporeal shockwave therapy (ESWT) on nerve regeneration are limited to the rat sciatic nerve model. The effects of ESWT on muscle-in-vein conduits (MVCs) have also not been investigated yet. This study aimed to evaluate the effects of ESWT after repair of the rat median nerve with either autografts (ANGs) or MVCs. In male Lewis rats, a 7 mm segment of the right median nerve was reconstructed either with an ANG or an MVC. For each reconstructive technique, one group of animals received one application of ESWT while the other rats served as controls. The animals were observed for 12 weeks, and nerve regeneration was assessed using computerized gait analysis, the grasping test, electrophysiological evaluations and histological quantification of axons, blood vessels and lymphatic vasculature. Here, we provide for the first time a comprehensive analysis of ESWT effects on nerve regeneration in a rat model of median nerve injury. Furthermore, this study is among the first reporting the quantification of lymphatic vessels following peripheral nerve injury and reconstruction in vivo. While we found no significant direct positive effects of ESWT on peripheral nerve regeneration, results following nerve repair with MVCs were significantly inferior to those after ANG repair.

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Appropriate Animal Models for Translational Nerve Research

Cited by 7 publications

References 79 publications

Perspective on Schwann Cells Derived from Induced Pluripotent Stem Cells in Peripheral Nerve Tissue Engineering

Perspective on Schwann Cells Derived from Induced Pluripotent Stem Cells in Peripheral Nerve Tissue Engineering

The Grasping Test Revisited: A Systematic Review of Functional Recovery in Rat Models of Median Nerve Injury

ESWT Diminishes Axonal Regeneration following Repair of the Rat Median Nerve with Muscle-In-Vein Conduits but Not after Autologous Nerve Grafting

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