Aptamer-based Colorimetric and Lateral Flow Chromatographic Strip detection of Aflatoxin B 1 in corn Samples

Abstract

In this paper, gold nanoparticles were combined with aflatoxin B1-aptamer as a single probe via the specific binding between aflatoxinB1 with aptamer, designing a colorimetric detection method. Meanwhile, a chromatographic strip with gold nanoparticles modified by complementary chains of aflatoxin B1 aptamer as the probe was developed for rapid detection of aflatoxin B1. Under the optimal conditions, the linear detection range of the colorimetric method was 0.5–50 ng/mL, the minimum detection limit was 0.5 ng/mL, the detection range of the chromatographic strip was 0.5–500 ng/mL, the minimum detection limit was 0.5 ng/ mL, and the semi-quantitative limit of detection was 51 ng/ml. Finally, comparing the two methods, it is concluded that chromatographic strip detection is more sensitive, accurate and celerity. Sample recovery rate of the latter was from 97.3–120%. All detections could be accomplished within 10 min, indicating that the test strip could be a potentially effective tool for rapid on-site detections.