Aptamer-based detection of thrombin by acoustic method using DNA tetrahedrons as immobilisation platform

Poturnayová, Alexandra; Šnejdárková, Maja; Castillo, G. F. Torres del; Rybár, Peter; Leitner, Michael; Ebner, Andreas; Hianik, Tibor

doi:10.1515/chempap-2015-0044

Cited by 6 publications

(2 citation statements)

References 71 publications

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“…improved TDN-based thrombin detection by functionalizing their nanostructure with two aptamers binding to different exosites of thrombin. 220 Detection of thrombin concentration was achieved using both cyclic voltammetry and the acoustic thickness shear mode (TSM) method. Voltammetry measurements were obtained using Ru(NH 3 ) 6…”

Section: Chemosensors and Biosensorsmentioning

confidence: 87%

“…Electrochemical sensing has also been combined with other detection techniques. Three years later, Poturnayová et al improved TDN-based thrombin detection by functionalizing their nanostructure with two aptamers binding to different exosites of thrombin . Detection of thrombin concentration was achieved using both cyclic voltammetry and the acoustic thickness shear mode (TSM) method.…”

Section: Applications Of Aptamers On Nucleic Acid Nanostructuresmentioning

confidence: 99%

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Self-Assembling Nucleic Acid Nanostructures Functionalized with Aptamers

et al. 2021

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Researchers have worked for many decades to master the rules of biomolecular design that would allow artificial biopolymer complexes to self-assemble and function similarly to the diverse biochemical constructs displayed in natural biological systems. The rules of nucleic acid assembly (dominated by Watson–Crick base-pairing) have been less difficult to understand and manipulate than the more complicated rules of protein folding. Therefore, nucleic acid nanotechnology has advanced more quickly than de novo protein design, and recent years have seen amazing progress in DNA and RNA design. By combining structural motifs with aptamers that act as affinity handles and add powerful molecular recognition capabilities, nucleic acid-based self-assemblies represent a diverse toolbox for use by bioengineers to create molecules with potentially revolutionary biological activities. In this review, we focus on the development of self-assembling nucleic acid nanostructures that are functionalized with nucleic acid aptamers and their great potential in wide ranging application areas.

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Section: Chemosensors and Biosensorsmentioning

confidence: 87%

Section: Applications Of Aptamers On Nucleic Acid Nanostructuresmentioning

confidence: 99%

Self-Assembling Nucleic Acid Nanostructures Functionalized with Aptamers

et al. 2021

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Electrochemical and Acoustic Biosensors Based on DNA Aptamers for Detection Mycotoxins

Hianik

2016

Biosensors for Security and Bioterrorism Applications

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Trends in the Design and Development of Specific Aptamers Against Peptides and Proteins

Tabarzad

Jafari

2016

Protein J

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Aptamers are single stranded oligonucleotides, comparable to monoclonal antibodies (mAbs) in selectivity and affinity and have significant strategic properties in design, development and applications more than mAbs. Ease of design and development, simple chemical modification and the attachment of functional groups, easily handling and more adaptability with analytical methods, small size and adaptation with nanostructures are the valuable characteristics of aptamers in comparison to large protein based ligands. Among a broad range of targets that their specific aptamers developed, proteins and peptides have significant position according to the number of related studies performed so far. Since proteins control many of important physiological and pathological incidents in the living organisms, particularly human beings and because of the benefits of aptamers in clinical and analytical applications, aptamer related technologies in the field of proteins and peptides are under progress, exclusively. Currently, there is only one FDA approved therapeutic aptamer in the pharmaceutical market, which is specific to vascular endothelial growth factor and is prescribed for age related macular degenerative disease. Additionally, there are several aptamers in the different phases of clinical trials. Almost all of these aptamers are specific to clinically important peptide or protein targets. In addition, the application of protein specific aptamers in the design and development of targeted drug delivery systems and diagnostic biosensors is another interesting field of aptamer technology. In this review, significant efforts related to development and applications of aptamer technologies in proteins and peptides sciences were considered to emphasis on the importance of aptamers in medicinal and clinical applications.

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Aptamer-based detection of thrombin by acoustic method using DNA tetrahedrons as immobilisation platform

Cited by 6 publications

References 71 publications

Self-Assembling Nucleic Acid Nanostructures Functionalized with Aptamers

Self-Assembling Nucleic Acid Nanostructures Functionalized with Aptamers

Electrochemical and Acoustic Biosensors Based on DNA Aptamers for Detection Mycotoxins

Trends in the Design and Development of Specific Aptamers Against Peptides and Proteins

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