Aptamer-based fluorescence biosensor for chloramphenicol determination using upconversion nanoparticles

Wu, Shijia; Zhang, Hui; Shi, Zhao; Duan, Nuo; Fang, Chong; Dai, Shaoliang; Wang, Zhouping

doi:10.1016/j.foodcont.2014.10.003

Cited by 203 publications

(61 citation statements)

References 39 publications

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“…It has been demonstrated that the aptamer interacts with its corresponding target with a greater binding constant compared to its complementary strand [23,25,26]. Upon addition of PG, a weak fluorescence emission is obtained, because of the presence of less dsDNA building blocks in the structure of disassembled DPN.…”

Section: Resultsmentioning

confidence: 99%

A fluorescent aptasensor based on a DNA pyramid nanostructure for ultrasensitive detection of ochratoxin A

et al. 2016

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Analytical techniques for detection of ochratoxin A (OTA) in food products and blood serum are of great significance. In this study, a fluorescent aptasensor was developed for sensitive and specific detection of OTA, based on a DNA pyramid nanostructure (DPN) and PicoGreen (PG) dye. The designed aptasensor inherits characteristics of DPN, such as high stability and capacity for PG loading. PG, as a fluorescent dye, could bind to double-stranded DNA (dsDNA). In the absence of OTA, the pyramid structure of DPN remains intact, leading to a very strong fluorescence emission. Because of higher affinity of aptamer for its target relative to its complementary strand, upon addition of target, the pyramid structure of DPN is disassembled, leading to a weak fluorescence emission. The presented aptasensor showed high specificity toward OTA with a limit of detection (LOD) as low as 0.135 nM. Besides, the designed sensing strategy was successfully utilized to recognize OTA in serum and grape juice with LODs of 0.184 and 0.149 nM, respectively.

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Section: Resultsmentioning

confidence: 99%

A fluorescent aptasensor based on a DNA pyramid nanostructure for ultrasensitive detection of ochratoxin A

et al. 2016

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“…On one hand the authors prepared mMSNs to which they grafted the 5'(H 2 N-CGA CCG TGG GAC AAC TCA)-3' anchor sequence; while on the other hand there were prepared, through avidin-biotin bridging, chloramphenicol aptamer 5'-(Biotin-AGC AGC ACA GAG GTC AGA TGA CTT CAG TGA GTT GTC CCA CGG TCG GCG AGT CGG TGG TAG CCT ATG CGT GCT ACC GTG AA)-3' functionalized upconversion nanoparticles. The combination of both MSNs and upconversion particles produced a magnetically decantable supramolecular construct sensitive to clorampenicol, 192 molecule that released of the traceable upconversion particles.…”

Section: Detection Of Small Moleculesmentioning

confidence: 99%

Recent applications of the combination of mesoporous silica nanoparticles with nucleic acids: development of bioresponsive devices, carriers and sensors

Castillo¹,

Baeza²

2017

Biomater. Sci.

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The discovery and control of the biological roles mediated by nucleic acids have turned them into a powerful tool for the development of advanced biotechnological materials. Much is the importance of those gene-keeping biomacromolecules that even nanomaterials have succumbed to the claimed benefits of DNA and RNA. Currently, there could be found in the literature a practically intractable number of examples which report the use in combination of nanoparticles with nucleic acids, which demands boundedness. Following this premise, this revision will only cover the most recent and powerful strategies developed to exploit the possibilities of nucleic acids as biotechnological materials when in combination with mesoporous silica nanoparticles. The extensive research done on nucleic acids has significantly incremented the technological possibilities for those biomacromolecules, which could be employed in many different applications; where substrate or sequence recognition or modulation of biological pathways due to its coding role in living cells are the most promising. In the present revision, the chosen counterpart, mesoporous silica nanoparticles, also with unique properties, became a reference material for drug delivery and biomedical applications due to their high biocompatibility and porous structure suitable for hosting and delivering small molecules. Although most of revisions deal with significant advances in the use of nucleic acid and mesoporous silica nanoparticles in biotechnological applications, a rationale classification of those new generation hybrid materials is still uncovered. Along this review there will be covered promising strategies for living cell and biological sensors, DNA-based molecular gates with targeting, transfection or silencing properties which could provide a significant advance of current nanomedicine.

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“…It has been demonstrated that aptamers bind to their targets with a greater binding constant compared to aptamers binding to their complimentary strands (Wu et al 2015;Yang et al 2014). So, addition of SWNTs on the surface of electrode, leads to the strong binding of SWNTs and CS (as a ssDNA) through π-π interactions (Taghdisi et al 2011).…”

Section: Sensing Schemementioning

confidence: 99%

“…Aptamers bind to their pre-selected targets, including small substances, proteins and even whole cells with high specificity and affinity (Wu et al 2015;Zhao et al 2015b). Compared to antibodies, aptamers exhibit unique properties such as lack of immunogenicity and toxicity, low cost, excellent thermal stability and ease of synthesis and modification (Eissa et al 2015;Lian et al 2015;Luo et al 2014;Zhao et al 2015a).…”

Section: Introductionmentioning

confidence: 99%

A novel electrochemical aptasensor based on single-walled carbon nanotubes, gold electrode and complimentary strand of aptamer for ultrasensitive detection of cocaine

Taghdisi

Danesh

Emrani

et al. 2015

Biosensors and Bioelectronics

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Aptamer-based fluorescence biosensor for chloramphenicol determination using upconversion nanoparticles

Cited by 203 publications

References 39 publications

A fluorescent aptasensor based on a DNA pyramid nanostructure for ultrasensitive detection of ochratoxin A

A fluorescent aptasensor based on a DNA pyramid nanostructure for ultrasensitive detection of ochratoxin A

Recent applications of the combination of mesoporous silica nanoparticles with nucleic acids: development of bioresponsive devices, carriers and sensors

A novel electrochemical aptasensor based on single-walled carbon nanotubes, gold electrode and complimentary strand of aptamer for ultrasensitive detection of cocaine

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