Aptamer-based sandwich assay for on chip detection of Ochratoxin A by an array of amorphous silicon photosensors

Costantini, Francesca; Sberna, Cristiana; Petrucci, Giulia; Reverberi, Massimo; Domenici, Fabio; Fanelli, C.; Manetti, Cesare; Cesare, G. de; DeRosa, Maria C.; Nascetti, A.; Caputo, D.

doi:10.1016/j.snb.2016.02.036

Cited by 51 publications

(46 citation statements)

References 37 publications

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“…Gold nanoparticles [77], dendrimers [78], quantum dots [79], and graphene oxide [80] have been employed in the mycotoxin sensing. In combination with nanoparticles, specific aptamers were also used to develop some fluorescence thin film sensors for mycotoxin detection [81,82]. The aptamer consists of a synthetic oligonucleotide ligand (either single stranded DNA (ssDNA) or RNA) that comprises less than 80 nucleotides and with less than 25 kDa and are known to exhibit high specificity and strong binding affinity [83].…”

Section: Fluorescence Sensorsmentioning

confidence: 99%

“…The interaction of this wave with free oscillating electrons at the metal film surface at a specific angle of incidence will cause the excitation of the plasmon surface, resulting in a decrease in the reflected light intensity. A SPR system thus detects changes in the refractive index of the surface layer of a solution in contact with the sensor chip [81]. Figure 2 (reproduced with permission from reference [91] ©Nature, 2018) shows the SPR biosensor principle, including a typical set-up as well as a common binding cycle.…”

Section: Surface Plasmon Resonance (Spr) Sensorsmentioning

confidence: 99%

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Thin Films Sensor Devices for Mycotoxins Detection in Foods: Applications and Challenges

et al. 2019

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Mycotoxins are a group of secondary metabolites produced by different species of filamentous fungi and pose serious threats to food safety due to their serious human and animal health impacts such as carcinogenic, teratogenic and hepatotoxic effects. Conventional methods for the detection of mycotoxins include gas chromatography and high-performance liquid chromatography coupled with mass spectrometry or other detectors (fluorescence or UV detection), thin layer chromatography and enzyme-linked immunosorbent assay. These techniques are generally straightforward and yield reliable results; however, they are time-consuming, require extensive preparation steps, use large-scale instruments, and consume large amounts of hazardous chemical reagents. Rapid detection of mycotoxins is becoming an increasingly important challenge for the food industry in order to effectively enforce regulations and ensure the safety of food and feed. In this sense, several studies have been done with the aim of developing strategies to detect mycotoxins using sensing devices that have high sensitivity and specificity, fast analysis, low cost and portability. The latter include the use of microarray chips, multiplex lateral flow, Surface Plasmon Resonance, Surface Enhanced Raman Scattering and biosensors using nanoparticles. In this perspective, thin film sensors have recently emerged as a good candidate technique to meet such requirements. This review summarizes the application and challenges of thin film sensor devices for detection of mycotoxins in food matrices.

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Section: Fluorescence Sensorsmentioning

confidence: 99%

Section: Surface Plasmon Resonance (Spr) Sensorsmentioning

confidence: 99%

Thin Films Sensor Devices for Mycotoxins Detection in Foods: Applications and Challenges

et al. 2019

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“…At this point, the PDMS stub, previously obtained, was bonded to the poly 2-(hydroxyethyl methacrylate) (PHEMA) functionalized with succinic anhydride functionalized glass substrate [23]. After the bonding between the PDMS serpentine channel and the PHEMA-functionalized glass slide, the microfluidic channel was further modified to immobilize streptavidin [7].…”

Section: Loc Structure and Fabricationmentioning

confidence: 99%

“…An alternative approach to antibodies, in biomolecular recognition, is represented by aptamers. Aptamers are artificial single-stranded DNA (ssDNA) or RNA sequences that fold into secondary and tertiary structures allowing their binding to selected targets with extremely high specificity [7], [8]. Due to their chemical structure, aptamers are much easier to be chemically modified with functional groups according to different purposes and are much more stable than antibodies.…”

mentioning

confidence: 99%

Integrated Sensor System for DNA Amplification and Separation Based on Thin Film Technology

Costantini

Petrucci

Lovecchio

et al. 2018

IEEE Trans. Compon., Packag. Manufact. Technol.

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This paper presents the development of a lab-onchip, based on thin-film sensors, suitable for DNA treatments. In particular, the system performs on-chip DNA amplification and separation of double-strand DNA into single-strand DNA, combining a polydimethylsiloxane microfluidic network, thin-film electronic devices, and surface chemistry. Both the analytical procedures rely on the integration on the same glass substrate of thinfilm metal heaters and amorphous silicon temperature sensors to achieve a uniform temperature distribution (within ±1°C) in the heated area and a precise temperature control (within ±0.5°C). The DNA separation also counts on the binding between biotinylated dsDNA and a layer of streptavidin immobilized into a microfluidic channel through polymer-brushes-based layer. This approach results in a fast and low reagents consumption system. The tested DNA treatments can be applied for carrying out the on-chip systematic evolution of ligands by exponential enrichment process, a chemistry technique for the selection of aptamers.Index Terms-Amorphous silicon sensor, lab-on-chip (LoC), systematic evolution of ligands by exponential enrichment (SELEX).

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“…2 . Further details on the photodiode array fabrication can be found in [47]. It can be deduced from the Figure 12 that the photocurrent initially decreases due to creation of two types of defects under blue light illumination.…”

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confidence: 99%

a-Si:H p-i-n Photodiode as a Biosensor

Gradišnik¹,

Gumbarević²

2018

Photodetectors [Working Title]

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The p-in a-Si:H photodiode is a promising device as a transducer in biosensors. The native and light-induced localized state density and energy distribution in the energy gap of a-Si:H have a large effect on the photoconductivity of thin-film photodiodes. Depending on their nature, they play a crucial role in trapping and recombination processes and consequently influence the photodiode capacitance. The optical bias dependence of modulated photocurrent, OBMPC, method using the blue LED light is applied to clarify the nature and energy distribution of the energy gap density of states and their influence on the photodiode capacitance, from photodiodes transient response. It is observed that the deep defect states of the i-layer contribute to the capacitance at various bias voltages. Also, the capacitance achieves the upper limit around the built-in potential. Based on this method and obtained results, the a-Si:H p-in photodiode is used as a biosensor transducer in the detection of mammalian cell chemiluminescence.

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Aptamer-based sandwich assay for on chip detection of Ochratoxin A by an array of amorphous silicon photosensors

Cited by 51 publications

References 37 publications

Thin Films Sensor Devices for Mycotoxins Detection in Foods: Applications and Challenges

Thin Films Sensor Devices for Mycotoxins Detection in Foods: Applications and Challenges

Integrated Sensor System for DNA Amplification and Separation Based on Thin Film Technology

a-Si:H p-i-n Photodiode as a Biosensor

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