Aptamer–gold nanoparticle conjugates for the colorimetric detection of arboviruses and vector mosquito species

Bosak, Alexander; Saraf, Nileshi; Willenberg, Alicia; Kwan, Michael W. C.; Alto, Barry W.; Jackson, Glen P.; Batchelor, Robert H.; Nguyen-Huu, Truong D.; Sankarapani, Vinoth; Parks, Griffith D.; Seal, Sudipta; Willenberg, Bradley J.

doi:10.1039/c9ra02089f

Cited by 42 publications

(25 citation statements)

References 36 publications

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“…Aedes aegypti eggs originally obtained from the United States Department of Agriculture-Agricultural Research Service, Center for Medical, Agricultural and Veterinary Entomology (USDA-ARS-CMAVE, Gainesville, FL) were continuously generated as previously described [45]. Briefly, eggs were collected and wrapped in Kimwipe tissue paper (Kimberly-Clark; Irving, TX) and kept in the dark within air-tight containers with a small cup of DI water.…”

Section: Mosquito Rearingmentioning

confidence: 99%

“…aegypti females were cold anaesthetized and sorted into groups of 50 for testing of adulticidal properties of nanoparticles. Mosquitoes were starved overnight on 5 DPE and fed nanoparticles within a previously developed sugar-based mosquito feeding solution (10% sucrose, 15mM NaCl, 1mM Na 2 CO 3 , 1μM MgCl 2 and 5μM ATP) [45]. Experimental conditions are outlined in Table 1.…”

Section: Adulticidal Properties Assessment Of Nanoparticlesmentioning

confidence: 99%

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Exposure to nanoceria impacts larval survival, life history traits and fecundity of Aedes aegypti

et al. 2020

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Effectively controlling vector mosquito populations while avoiding the development of resistance remains a prevalent and increasing obstacle to integrated vector management. Although, metallic nanoparticles have previously shown promise in controlling larval populations via mechanisms which are less likely to spur resistance, the impacts of such particles on life history traits and fecundity of mosquitoes are understudied. Herein, we investigate the chemically well-defined cerium oxide nanoparticles (CNPs) and silver-doped nanoceria (AgCNPs) for larvicidal potential and effects on life history traits and fecundity of Aedes (Ae.) aegypti mosquitoes. When 3 rd instar larvae were exposed to nanoceria in absence of larval food, the mortality count disclosed significant activity of AgCNPs over CNPs (57.8 ±3.68% and 17.2±2.81% lethality, respectively) and a comparable activity to Ag + controls (62.8±3.60% lethality). The surviving larvae showed altered life history traits (e.g., reduced egg hatch proportion and varied sex ratios), indicating activities of these nanoceria beyond just that of a larvicide. In a separate set of experiments, impacts on oocyte growth and egg generation resulting from nanoceria-laced blood meals were studied using confocal fluorescence microscopy revealing oocytes growth-arrest at 16-24h after feeding with AgCNPblood meals in some mosquitoes, thereby significantly reducing average egg clutch. AgCNPs caused~60% mortality in 3 rd instar larvae when larval food was absent, while CNPs yielded only~20% mortality which contrasts with a previous report on green-synthesized nanoceria and highlights the level of detail required to accurately report and interpret such studies. Additionally, AgCNPs are estimated to contain much less silver (0.22 parts per billion, ppb) than the amount of Ag + needed to achieve comparable larvicidal activity (2.7 parts per million, ppm), potentially making these nanoceria ecofriendly. Finally, this work is the first study to demonstrate the until-now-unappreciated impacts of nanoceria on life history traits and interference with mosquito egg development.

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Section: Mosquito Rearingmentioning

confidence: 99%

Section: Adulticidal Properties Assessment Of Nanoparticlesmentioning

confidence: 99%

Exposure to nanoceria impacts larval survival, life history traits and fecundity of Aedes aegypti

et al. 2020

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“…Aedes aegypti eggs were obtained from the United States Department of Agriculture-Agricultural Research Service, Center for Medical, Agricultural and Veterinary Entomology (USDA-ARS-CMAVE, Gainesville, FL) and continued to be cultivated in the Willenberg Lab as described elsewhere 48 . Briefly, 8 mg of eggs ( eggs) are brushed off the cards and shaken vigorously in a glass vial containing 7.5 mL of larval food in deionized (DI) water (40:60; brewer’s yeast and liver powder).…”

Section: Experimental Methodsmentioning

confidence: 99%

Landing mosquitoes bounce when engaging a substrate

Smith

Balsalobre

Doshi

et al. 2020

Sci Rep

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In this experimental study we film the landings of Aedes aegypti mosquitoes to characterize landing behaviors and kinetics, limitations, and the passive physiological mechanics they employ to land on a vertical surface. A typical landing involves 1–2 bounces, reducing inbound momentum by more than half before the mosquito firmly attaches to a surface. Mosquitoes initially approach landing surfaces at 0.1–0.6 m/s, decelerating to zero velocity in approximately 5 ms at accelerations as high as 5.5 gravities. Unlike Dipteran relatives, mosquitoes do not visibly prepare for landing with leg adjustments or body pitching. Instead mosquitoes rely on damping by deforming two forelimbs and buckling of the proboscis, which also serves to distribute the impact force, lessening the potential of detection by a mammalian host. The rebound response of a landing mosquito is well-characterized by a passive mass-spring-damper model which permits the calculation of force across impact velocity. The landing force of the average mosquito in our study is approximately 40 $$\upmu$$ μ N corresponding to an impact velocity of 0.24 m/s. The substrate contact velocity which produces a force perceptible to humans, 0.42 m/s, is above 85% of experimentally observed landing speeds.

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“…This work achieved qualitative sensing of OTC with an LOD B 25 nM. Sensitive detection of other targets such as pesticides, 140,141 arboviruses, 142 exosome surface proteins, 130 and organic synthetic compounds 143 has also been achieved.…”

Section: Solution-based Aptasensormentioning

confidence: 96%

Detection and beyond: challenges and advances in aptamer-based biosensors

Yoo

2020

Mater. Adv.

189

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Aptamer–gold nanoparticle conjugates for the colorimetric detection of arboviruses and vector mosquito species

Abstract: Colorimetric detection of arboviruses and vector mosquito species. (A) Aptamer-gold nanoparticle conjugates, (B) mosquito salivary protein, (C) Zika envelope protein, (D) visible color change, (E) imbibed diagnostic and (F) diagnostic cards.

Cited by 42 publications

References 36 publications

Exposure to nanoceria impacts larval survival, life history traits and fecundity of Aedes aegypti

Exposure to nanoceria impacts larval survival, life history traits and fecundity of Aedes aegypti

Landing mosquitoes bounce when engaging a substrate

Detection and beyond: challenges and advances in aptamer-based biosensors

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