Aptamer/Hydroxyapatite-Functionalized Titanium Substrate Promotes Implant Osseointegration via Recruiting Mesenchymal Stem Cells

Abstract

Endowing bone regeneration materials with both stem cell recruitment and osteoinduction properties is a key factor in promoting osseointegration of titanium (Ti) implants. In this study, Apt19s-grafted oxidized hyaluronic acid (OHA) was deposited onto a protein-mediated biomineralization hydroxyapatite (HAp) coating of Ti. HAp was achieved by the treatment of lysozyme and tris­(2-carboxyethyl) phosphonate mixture and then soaked in calcium ion (Ca2+) solution to obtain functional Ti substrate (Ti/HAp/OHA-Apt). In vitro studies confirmed that Ti/HAp/OHA-Apt could effectively maintain the sustained release of Apt19s from Ti for 7 days. The released Apt19s significantly enhanced the migration of bone marrow mesenchymal stem cells (MSCs), which was reflected by the experiment of transwell assay, wound healing, and zymogram detection. Compared with pure Ti, Ti/HAp/OHA-Apt was able to adjust the adsorption of functional proteins at the Ti-based interface to expose their active sites, which significantly increased the expression of adhesion-associated proteins (vinculin and tensin) in MSCs to promote their adhesion on Ti-based interface. In vitro cell experiments of alkaline phosphatase activity staining, mineralization detection, and expression of osteogenesis-related genes showed that Ti/HAp/OHA-Apt significantly enhanced the osteogenic differentiation ability of MSCs, which may be highly related to the porous structure of hydroxyapatite on Ti interface. In vivo test of Micro-CT, H&E staining, and histochemical staining further confirmed that Ti/HAp/OHA-Apt was able to promote MSC recruitment at the peri-implant interface to form new bone. This work provides a new approach to develop functional Ti-based materials for bone defect repair.

Bisphosphonate-Modified Functional Supramolecular Hydrogel Promotes Periodontal Bone Regeneration by Osteoclast Inhibition

“…Although nanoscale HAP has a large specific surface area and good biodegradability, its combination with Nap-Alen hydrogels provided less improved mechanical and mechanical properties than porous micron HAP; the in vivo migration and safety of nanoparticles need to be further validated by more studies. A significant number of proteins and cytokines can be densely loaded into the porous, sparse 3D structure of micron HAP, which also encourages the migration of additional functional cells . Therefore, we selected a composite hydrogel material (Nap-Alen/HAP) containing 2 wt % micron-scale HAP as the material used in the subsequent experiments.…”

HE@PCL/PCE Gel-Nanofiber Dressing with Robust Self-Adhesion toward High Wound-Healing Rate via Microfluidic Electrospinning Technology

“…Also, this evidence indicates the successful grafting of −CHO groups. More importantly, according to the specific signal peak areas of aldehydes and methyl groups, oxidation degree = integral of aldehyde groups/integral of methyl groups, 39 the oxidation degrees of OHA-2, OHA-4, and OHA-8 h were calculated as 7.2, 18.2, and 25.6%, respectively. In fact, the degree of oxidation is closely related to the aldehyde content.…”

Enhancing Bone Regeneration through CDC20‐Loaded ZIF‐8 Nanoparticles Wrapped in Erythrocyte Membranes with Targeting Aptamer

“…The BMSCspecific aptamer called Apt19S was introduced and the sequences were shown in Table S1, Supporting Information. [42,70,71] BMSCs were isolated from the bone marrow of rats. Healthy Sprague-Dawley (SD) rats were purchased from the Hubei Provincial Academy of Preventive Medicine (Wuhan, China).…”