Aptamer in Bioanalytical Applications

Iliuk, Anton; Hu, Lianghai; Tao, W. Andy

doi:10.1021/ac201057w

Cited by 710 publications

(454 citation statements)

References 173 publications

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“…The SELEX process for production of aptamers offers the advantage over natural antibodies that the time required for aptamers' selection is only a few weeks rather than a few months needed to produce monoclonal antibodies [121]. Aptamers are also much more stable than antibodies making them suitable in applications requiring harsh conditions, (e.g., high temperature or extreme pH) [143]. General review articles about aptamer-based sensors [144] and aptamer-based sensors for viruses are available in literature [145][146][147].…”

Section: Aptamersmentioning

confidence: 99%

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

et al. 2017

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Viruses are pathogenic microorganisms that can inhabit and replicate in human bodies causing a number of widespread infectious diseases such as influenza, gastroenteritis, hepatitis, meningitis, pneumonia, acquired immune deficiency syndrome (AIDS) etc. A majority of these viral diseases are contagious and can spread from infected to healthy human beings. The most important step in the treatment of these contagious diseases and to prevent their unwanted spread is to timely detect the disease-causing viruses. Gravimetric viral diagnostics based on quartz crystal microbalance (QCM) transducers and natural or synthetic receptors are miniaturized sensing platforms that can selectively recognize and quantify harmful virus species. Herein, a review of the label-free QCM virus sensors for clinical diagnostics and point of care (POC) applications is presented with major emphasis on the nature and performance of different receptors ranging from the natural or synthetic antibodies to selective macromolecular materials such as DNA and aptamers. A performance comparison of different receptors is provided and their limitations are discussed.

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Section: Aptamersmentioning

confidence: 99%

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

et al. 2017

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“…On the one hand, aptamers afford a novel recognizing platform for biochemical analysis. [11] With the development of study, aptamers have been proved as superior specific units for a majority of targets including peptides, [12] small molecules, [13] and even cancer cells. [14] And aptamers can be designed for other forms for the sake of signal transformation of target.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic Paper‐Based Analytical Device for Sensitive Detection of Peptides Based on Specific Recognition of Aptamer and Amplification Strategy of Hybridization Chain Reaction

Liu

Zhang

et al. 2017

ChemElectroChem

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In this paper, a microfluidic paper-based analytical device is presented for sensitively detecting peptides based on the specific recognition of aptamer and the amplification strategy of hybridization chain reaction (HCR). The aptamer/initiation strand (AP/IS) was designed for the specific target recognition, and the presence of the target peptide could release the initiating sequence from the AP/IS. The released IS could trigger the HCR between two hairpin probes, which could complete the detection for the targets indirectly when the electrochemiluminescence (ECL) probe (Ru(phen) 3 2 + ) is introduced into the double-strand DNA grooves,which could produce a significantly amplified ECL signal output. Mucin-1 was employed as a model peptide, and the detection limit of the proposed assay is calculated as 8.33 pMwith a range from 25 pM to 50 nM. The novel sensing platform can be widened for the detection of other kinds of peptides as well as potentials used for sensitively detecting other biomolecules, and even cells.

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“…Aptamers have great potential in therapy (Keefe et al, 2010), diagnosis (Brody et al, 2010), in vitro bioanalysis (Iliuk et al, 2011) and in vivo imaging (Hong et al, 2011;Lopez-Colon et al, 2011). The combination of aptamer with nanotechnology is also an actively studied field .…”

Section: Introductionmentioning

confidence: 99%

Aptamer-based molecular imaging

Wang

Ray

2012

Protein Cell

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Molecular imaging has greatly advanced basic biology and translational medicine through visualization and quantification of single/multiple molecular events temporally and spatially in a cellular context and in living organisms. Aptamers, short single-stranded nucleic acids selected in vitro to bind a broad range of target molecules avidly and specifically, are ideal molecular recognition elements for probe development in molecular imaging. This review summarizes the current state of aptamer-based biosensor development (probe design and imaging modalities) and their application in imaging small molecules, nucleic acids and proteins mostly in a cellular context with some animal studies. The article is concluded with a brief discussion on the perspective of aptamer-based molecular imaging.

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Aptamer in Bioanalytical Applications

Cited by 710 publications

References 173 publications

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

Microfluidic Paper‐Based Analytical Device for Sensitive Detection of Peptides Based on Specific Recognition of Aptamer and Amplification Strategy of Hybridization Chain Reaction

Aptamer-based molecular imaging

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