Aptamer recognition and proximity-induced entropy-driven circuit for enzyme-free and rapid amplified detection of platelet-derived growth factor-BB

Li, Dandan; Li, Xinmin; Shen, Bo; Li, Pu; Chen, Yuanjiao; Ding, Shijia; Chen, Weixian

doi:10.1016/j.aca.2019.09.046

Cited by 20 publications

(2 citation statements)

References 39 publications

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“…In addition to the detection of target nucleic acids, including particular genes of interest, mRNA, and miRNA, 27 the system can be used for electrochemical sensing of other molecules that replace a strand from a tandem duplex carrying a cognate nucleic acid aptamer. 28 Synthetic scheme for the conjugates. RP-HPLC chromatograms for the isolation of F and Q after each synthesis.…”

Section: Discussionmentioning

confidence: 99%

Catalytic Amplification of Electrochemical Signal in Homogeneous Solution Using an Entropy-driven DNA Circuit

et al. 2020

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The electrochemical signal from ferrocene on a DNA probe was successfully modulated in a homogeneous solution by template-directed formation and dissociation of an inclusion complex with β-cyclodextrin on another probe. The electrochemical response was amplified by combination with a DNA circuit, in which the target DNA served as a catalyst. This system did not require modification of a complementary DNA with the ferrocene-modified probe on the electrode surface to separate the bound/free probe for detection of 200 nM target DNA.

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Section: Discussionmentioning

confidence: 99%

Catalytic Amplification of Electrochemical Signal in Homogeneous Solution Using an Entropy-driven DNA Circuit

et al. 2020

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“…The PHA has demonstrated excellent specificity in biosensing, due to the co-recognition of the double-probe system. Consequently, in recent years, PHA has been extensively explored for protein detection, including platelet derived growth factor-BB (PDGF-BB), − thrombin, − alpha-fetoprotein (AFP), , cystain C, prostate-specific antigen (PSA), carcinoembryonic antigen (CEA) , and human epididymis-specific protein 4 (HE4) …”

Section: Introductionmentioning

confidence: 99%

Triblock PolyA-Mediated Protein Biosensor Based on a Size-Matching Proximity Hybridization Analysis

Chen,

Wen,

Wang

et al. 2024

Anal. Chem.

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The antibodies in the natural biological world utilize bivalency/multivalency to achieve a higher affinity for antigen capture. However, mimicking this mechanism on the electrochemical sensing interface and enhancing biological affinity through precise spatial arrangement of bivalent aptamer probes still pose a challenge. In this study, we have developed a novel self-assembly layer (SAM) incorporating triblock polyA DNA to enable accurate organization of the aptamer probes on the interface, constructing a “lock-and-key-like” proximity hybridization assay (PHA) biosensor. The polyA fragment acts as an anchoring block with a strong affinity for the gold surface. Importantly, it connects the two DNA probes, facilitating one-to-one spatial proximity and enabling a controllable surface arrangement. By precisely adjusting the length of the polyA fragment, we can tailor the distance between the probes to match the molecular dimensions of the target protein. This design effectively enhances the affinity of the aptamers. Notably, our biosensor demonstrates exceptional specificity and sensitivity in detecting PDGF-BB, as confirmed through successful validation using human serum samples. Overall, our biosensor presents a novel and versatile interface for proximity assays, offering a significantly improved surface arrangement and detection performance.

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Multiply Guaranteed and Successively Amplified Activation of a Catalytic DNA Machine for Highly Efficient Intracellular Imaging of MicroRNA

Shang

et al. 2022

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DNA amplification machines show great promise for intracellular imaging, yet are always constrained by off‐site machinery activation or signal leakage, originating from the inherent thermodynamically driven hybridization between machinery substrates. Herein, an entropy‐driven catalytic DNA amplification machine is integrated with the on‐site amplified substrate exposure procedure to realize the high‐contrast in vivo imaging of microRNA (miRNA). The key machinery substrate (fuel strands) is initially split into substrate subunits that are respectively grafted into an auxiliary DNA polymerization amplification accessory for eliminating the undesired signal leakage. Meanwhile, in target cells, the auxiliary polymerization accessory can be motivated by cell‐specific mRNA for successively restoring their intact machine‐propelling functions for guaranteeing the on‐site amplified imaging of miRNA with high specificity. This intelligent on‐site multiply guaranteed machinery can improve the specificity of catalytic DNA machines for discriminating different cell types and, thus, can provide a remarkable prospect in biomedical diagnosis.

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Aptamer recognition and proximity-induced entropy-driven circuit for enzyme-free and rapid amplified detection of platelet-derived growth factor-BB

Cited by 20 publications

References 39 publications

Catalytic Amplification of Electrochemical Signal in Homogeneous Solution Using an Entropy-driven DNA Circuit

Catalytic Amplification of Electrochemical Signal in Homogeneous Solution Using an Entropy-driven DNA Circuit

Triblock PolyA-Mediated Protein Biosensor Based on a Size-Matching Proximity Hybridization Analysis

Multiply Guaranteed and Successively Amplified Activation of a Catalytic DNA Machine for Highly Efficient Intracellular Imaging of MicroRNA

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