Aptamers: a new class of oligonucleotides in the drug discovery pipeline?

Dausse, Eric; Gomes, Sonia Da Rocha; Toulmé, Jean‐Jacques

doi:10.1016/j.coph.2009.07.006

Cited by 70 publications

(43 citation statements)

References 49 publications

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“…The specificity of the strongest ligand, H19, may be used to distinguish clinical isolates of S. mutans with high and low cariogenicity [55]. The dissociation constant we determined for Aptamer H19 and the cariogenic Strain 17 was of nM(nmol) order, similar to that reported in a previous study in whole cell targets using SELEX [50].…”

Section: Discussionsupporting

confidence: 70%

Identification of ssDNA aptamers specific to clinical isolates of <italic>Streptococcus mutans</italic> strains with different cariogenicity

Cui¹,

Liu²,

Su³

et al. 2016

ABBS

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Streptococcus mutans, a Gram-positive facultative anaerobic bacterium, is considered to be a major etiological factor for dental caries. In this study, plaques from dental enamel surfaces of caries-active and caries-free individuals were obtained and cultivated for S. mutans isolation. Morphology examination, biochemical characterization, and polymerase chain reaction were performed to identify S. mutans. The cariogenicity of S. mutans strains isolated from clinical specimens was evaluated by testing the acidogenicity, aciduricity, extracellular polysaccharide production, and adhesion ability of the bacteria. Finally, subtractive SELEX (systematic evolution of ligands by exponential enrichment) technology targeting whole intact cells was used to screen for ssDNA aptamers specific to the strains with high cariogenicity. After nine rounds of subtractive SELEX, sufficient pool enrichment was achieved as shown by radioactive isotope analysis. The enriched pool was cloned and sequenced randomly, followed by MEME online and RNA structure software analysis of the sequences. Results from the flow cytometry indicated that aptamers H1, H16, H4, L1, L10, and H19 could discriminate highly cariogenic S. mutans strains from poorly cariogenic strains. Among these, Aptamer H19 had the strongest binding capacity with cariogenic S. mutans strains with a dissociation constant of 69.45 ± 38.53 nM. In conclusion, ssDNA aptamers specific to highly cariogenic clinical S. mutans strains were successfully obtained. These ssDNA aptamers might be used for the early diagnosis and treatment of dental caries.

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Section: Discussionsupporting

confidence: 70%

Identification of ssDNA aptamers specific to clinical isolates of <italic>Streptococcus mutans</italic> strains with different cariogenicity

Cui¹,

Liu²,

Su³

et al. 2016

ABBS

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“…101,102 Aptamers are short single-stranded DNA, RNA, or modified nucleic acids that can fold into complex three-dimensional structures and specifically bind to their targets. 103 Aptamers can be selected from randomly synthesized oligonucleotide libraries through the systematic evolution of ligands by exponential enrichment. 104 Aptamers have been demonstrated to have more specific and higher affinity for the intended target than other ligands (eg, antibodies).…”

Section: Aptamersmentioning

confidence: 99%

How nanotechnology can enhance docetaxel therapy

Zhang¹,

Zhang²

2013

IJN

101

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Docetaxel has been recognized as one of the most efficient anticancer drugs over the past decade; however, its poor water solubility and systemic toxicity have greatly limited its clinical application. In recent decades, the emergence of nanotechnology has provided new drug delivery systems for docetaxel, which can improve its water solubility, minimize the side effects and increase the tumor-targeting distribution by passive or active targeting. This review focuses on the research progress in nanoformulations related to docetaxel delivery -such as polymer-based, lipid-based, and lipid-polymer hybrid nanocarriers, as well as inorganic nanoparticles -addressing their structures, characteristics, preparation, physicochemical properties, methods by which drugs are loaded into them, and their in vitro and in vivo efficacies. Further, the targeted ligands used in the docetaxel nanoformulations, such as monoclonal antibodies, peptides, folic acid, transferrin, aptamers and hyaluronic acid, are described. The issues to overcome before docetaxel nanoformulations can be used in clinical and commercial applications are also discussed.

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“…Gene silencing siRNAs have been linked to aptamers to specifically deliver siRNAs to target cells, thus removing one of the limitations of using siRNA. This has been successfully demonstrated with a prostate specific membrane antigen (PSMA) aptamer delivering siRNA against prosurvival genes [16,39,40]. PSMA has also been directly conjugated to a chemotherapeutic drug, doxorubicin.…”

Section: Aptamer Conjugationmentioning

confidence: 99%

Aptamer Therapeutics: The 21st Century`s Magic Bullet of Nanomedicine

Shigdar¹,

Luczo²,

Wei³

et al. 2010

TOPROCJ

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Aptamers, also known as chemical antibodies, are short single-stranded DNA, RNA or peptide molecules. These molecules can fold into complex three-dimensional structures and bind to target molecules with high affinity and specificity. The nucleic acid aptamers are selected from combinatorial libraries by an iterative in vitro selection procedure known as systematic evolution of ligands by exponential enrichment (SELEX). As a new class of therapeutics and drug targeting entities, bivalent and multivalent aptamer-based molecules are emerging as highly attractive alternatives to monoclonal antibodies as targeted therapeutics.Aptamers have several advantages, offering the possibility of overcoming limitations of antibodies: 1) they can be selected against toxic or non-immunogenic targets; 2) aptamers can be chemically modified by using modified nucleotides to enhance their stability in biological fluids or via incorporating reporter molecules, radioisotopes and functional groups for their detection and immobilization; 3) they have very low immunogenicity; 4) they display high stability at room temperature, in extreme pH, or solvent; 5) once selected, they can be chemically synthesized free from cell-culturederived contaminants, and they can be manufactured at any time, in large amounts, at relatively low cost and reproducibly; 6) they are smaller and thus can diffuse more rapidly into tissues and organs, leading to faster targeting in drug delivery; 7) they have lower molecular weight that can lead to faster body clearance, resulting in a low background noise for imaging and minimizing the radiation dose to the patient in diagnostic imaging. Thus, the high selectivity and sensitivity, ease of screening and production, chemical versatility as well as stability make aptamers a class of highly attractive agents for the development of novel therapeutics, targeted drug delivery vehicles and molecular imaging.In the review, we will discuss the latest technological advances in developing aptamers, its application as a novel class of drug on its own, as well as in surface functionalization of both polymer nanoparticles or nanoliposomes in the treatment of cancer, viral and autoimmune diseases.

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Aptamers: a new class of oligonucleotides in the drug discovery pipeline?

Cited by 70 publications

References 49 publications

Identification of ssDNA aptamers specific to clinical isolates of <italic>Streptococcus mutans</italic> strains with different cariogenicity

Identification of ssDNA aptamers specific to clinical isolates of <italic>Streptococcus mutans</italic> strains with different cariogenicity

How nanotechnology can enhance docetaxel therapy

Aptamer Therapeutics: The 21st Century`s Magic Bullet of Nanomedicine

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Aptamers: a new class of oligonucleotides in the drug discovery pipeline?

Cited by 70 publications

References 49 publications

Identification of ssDNA aptamers specific to clinical isolates of &lt;italic&gt;Streptococcus mutans&lt;/italic&gt; strains with different cariogenicity

Identification of ssDNA aptamers specific to clinical isolates of &lt;italic&gt;Streptococcus mutans&lt;/italic&gt; strains with different cariogenicity

How nanotechnology can enhance docetaxel therapy

Aptamer Therapeutics: The 21st Century`s Magic Bullet of Nanomedicine

Contact Info

Product

Resources

About

Identification of ssDNA aptamers specific to clinical isolates of <italic>Streptococcus mutans</italic> strains with different cariogenicity

Identification of ssDNA aptamers specific to clinical isolates of <italic>Streptococcus mutans</italic> strains with different cariogenicity