Aptamers as smart ligands for nano-carriers targeting

Mokhtarzadeh, Ahad; Tabarzad, Maryam; Ranjbari, Javad; Guárdia, Miguel de la; Hejazi, Maryam; Ramezani, Mohammad

doi:10.1016/j.trac.2016.06.018

Cited by 56 publications

(18 citation statements)

References 159 publications

(101 reference statements)

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“…SELEX referred to as in vitro evolution or in vitro selection is a combinatorial chemistry method in molecular biology for the screening of single-stranded DNA or RNA that specifically binds to a target ligand or ligands with high affinity [ 164 , 165 ]. Because of the benefits of better stability, simple modification, and easy preparation, aptamers were utilized to fabricate biosensors for detecting infectious microorganisms.…”

Section: Detection Methodsmentioning

confidence: 99%

An Overview on SARS-CoV-2 (COVID-19) and Other Human Coronaviruses and Their Detection Capability via Amplification Assay, Chemical Sensing, Biosensing, Immunosensing, and Clinical Assays

et al. 2020

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A novel coronavirus of zoonotic origin (SARS-CoV-2) has recently been recognized in patients with acute respiratory disease. COVID-19 causative agent is structurally and genetically similar to SARS and bat SARS-like coronaviruses. The drastic increase in the number of coronavirus and its genome sequence have given us an unprecedented opportunity to perform bioinformatics and genomics analysis on this class of viruses. Clinical tests like PCR and ELISA for rapid detection of this virus are urgently needed for early identification of infected patients. However, these techniques are expensive and not readily available for point-of-care (POC) applications. Currently, lack of any rapid, available, and reliable POC detection method gives rise to the progression of COVID-19 as a horrible global problem. To solve the negative features of clinical investigation, we provide a brief introduction of the general features of coronaviruses and describe various amplification assays, sensing, biosensing, immunosensing, and aptasensing for the determination of various groups of coronaviruses applied as a template for the detection of SARS-CoV-2. All sensing and biosensing techniques developed for the determination of various classes of coronaviruses are useful to recognize the newly immerged coronavirus, i.e., SARS-CoV-2. Also, the introduction of sensing and biosensing methods sheds light on the way of designing a proper screening system to detect the virus at the early stage of infection to tranquilize the speed and vastity of spreading. Among other approaches investigated among molecular approaches and PCR or recognition of viral diseases, LAMP-based methods and LFAs are of great importance for their numerous benefits, which can be helpful to design a universal platform for detection of future emerging pathogenic viruses.

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Section: Detection Methodsmentioning

confidence: 99%

An Overview on SARS-CoV-2 (COVID-19) and Other Human Coronaviruses and Their Detection Capability via Amplification Assay, Chemical Sensing, Biosensing, Immunosensing, and Clinical Assays

et al. 2020

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“…Specifically, future studies have to consider potential nuclear degradation of aptamers, if they are to be delivered to live organisms. Nevertheless, studies focusing on chemical modifications of aptamers or aptamer conjugation to nanomaterials can increase their stability and limit any enzymatic action . Importantly, the combination of nucleic acid aptamers and drugs has been used to treat specific diseases .…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, studies focusing on chemical modifications of aptamers or aptamer conjugation to nanomaterials can increase their stability and limit any enzymatic action. 46,47 Importantly, the combination of nucleic acid aptamers and drugs has been used to treat specific diseases. 48 The development of aptamer drugs will likely provide a new platform for the disease diagnosis, and perhaps even in the treatment of cancer, and is expected to significantly improve human health.…”

Section: Hp5 Gggctgtgggtcggcacgtcgtctcttcatggttgtggtgmentioning

confidence: 99%

Recognition of Helicobacter pylori by protein‐targeting aptamers

Yan

Ren

et al. 2019

Helicobacter

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Background Helicobacter pylori (H pylori) is a disease‐causing pathogen capable of surviving under acidic conditions of the human stomach. Almost half of the world's population is infected with H pylori, with gastric cancer being the most unsatisfactory prognosis. Although H pylori has been discovered 30 years ago, the effective treatment and elimination of H pylori continue to be problematic. Materials and methods In our study, we screened nucleic acid aptamers using H pylori surface recombinant antigens as targets. Trypsin was used for separating aptamers that were bound to proteins. Following nine rounds of screening, we performed sequence similarity analyses to assess whether the aptamers can recognize the target protein. Two sequences with desirable recognition ability were selected for affinity detection. Aptamer Hp4 with the strongest binding ability to the H pylori surface recombinant antigen was chosen. After optimization of the binding conditions, we conducted specificity tests for Hp4 using Escherichia coli, Staphylococcus aureus, Vibrioanguillarum, and H pylori. Results The data indicated that the aptamer Hp4 had an equilibrium dissociation constant (Kd) of 26.48 ± 5.72 nmol/L to the target protein. This aptamer was capable of exclusively detecting H pylori cells, without displaying any specificity for other bacteria. Conclusions We obtained a high‐affinity aptamer for H pylori, which is expected to serve as a new molecular probe for detection of H pylori.

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“…Additionally, nano-aptamer-drug delivery system enables the controlled release of loaded drug, improves drug bioavailability. 18 Aptamers as molecular probes should efficiently recognize cancer cell, and directly deliver chemotherapeutic drug to tumor cells. In following, we discuss applications of aptamer–nanomaterial complexes in CRC therapy.…”

Section: Aptamer-based Targeted Therapy For Crcmentioning

confidence: 99%

“… 15 – 17 They have remarkable characteristics including chemical properties and controllable physical, high stability, and high carrier capacity. 18 Herein, we focus on Aptamer application in CRC diagnosis and therapy, andaptamer-conjugated nanoparticles for CRC targeted drug delivery.…”

Section: Introductionmentioning

confidence: 99%

Nucleic acid aptamer application in diagnosis and therapy of colorectal cancer based on cell-SELEX technology

et al. 2017

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Nucleic acid aptamers are a class of high-affinity nucleic acid ligands. They serve as “chemical antibodies” since their high affinity and specificity. Nucleic acid aptamers are generated from nucleic acid random-sequence using a systematic evolution of ligands by exponential enrichment (SELEX) technology. SELEX is a process of effectively selecting aptamers from different targets. A newly developed cell-based SELEX technique has been widely used in biomarker discovery, early diagnosis and targeted cancer therapy, particular at colorectal cancer (CRC). Combined with nanostructures, nano-aptamer-drug delivery system was constructed for drug delivery. Various nanostructures functionalized with aptamers are highly efficient and has been used in CRC therapeutic applications. In the present, we introduce a cell- SELEX technique, and summarize the potential application of aptamers as biomarkers in CRC diagnosis and therapy. And some characteristics of aptamer-targeted nanocarriers in CRC have been expatiated. The challenges and perspectives for cell-SELEX are also discussed.

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Aptamers as smart ligands for nano-carriers targeting

Cited by 56 publications

References 159 publications

An Overview on SARS-CoV-2 (COVID-19) and Other Human Coronaviruses and Their Detection Capability via Amplification Assay, Chemical Sensing, Biosensing, Immunosensing, and Clinical Assays

An Overview on SARS-CoV-2 (COVID-19) and Other Human Coronaviruses and Their Detection Capability via Amplification Assay, Chemical Sensing, Biosensing, Immunosensing, and Clinical Assays

Recognition of Helicobacter pylori by protein‐targeting aptamers

Nucleic acid aptamer application in diagnosis and therapy of colorectal cancer based on cell-SELEX technology

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