APTC-C-SA01: A Novel Bacteriophage Cocktail Targeting Staphylococcus aureus and MRSA Biofilms

Liu, Sha; Hon, Karen; Bouras, George; Psaltis, Alkis James; Shearwin, Keith E.; Wormald, Peter‐John; Vreugde, Sarah

doi:10.3390/ijms23116116

Cited by 13 publications

(10 citation statements)

References 50 publications

(55 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The P. aeruginosa phage APTC-PA18 is a lytic phage isolated from a DFU P. aeruginosa infection, which is a common occurrence, as lytic phages are frequently isolated from active infection sites in humans 19 . APTC-PA18 was shown to have desirable properties that make it an excellent clinical phage candidate as it lacked lysogeny-associated and toxin-encoding genes.…”

Section: Discussionmentioning

confidence: 99%

Bacteriophage in combination with ciprofloxacin againstPseudomonas aeruginosainfections in diabetic foot ulcer patients

Liu,

Goonetilleke,

Hon

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

BackgroundIn diabetic foot ulcer (DFU) patients,Pseudomonas aeruginosa(P. aeruginosa) infections are linked to poor wound healing. The ineffectiveness of antibiotics against these infections promotes the emergence of multidrug-resistant (MDR) strains. Bacteriophage (phage) therapy has recently gained popularity as an alternative to antibiotics.MethodologyBacterial and viral swabs and tissue were obtained from DFU infections (DFI). Bacteria were cultured followed by MALDI-TOF MS for identification. 16S rRNA long-read sequencing was used to identify the microbiota. Bacteriophages were isolated and underwent transmission electron microscopy, genomic sequencing, and stability testing. The antimicrobial activity of phages alone and in combination with ciprofloxacin againstP. aeruginosaplanktonic cells and biofilm grownin vitroand inex vivotissue was tested by measuring the optical density (OD), crystal violet assays and live/dead staining with visualisation using confocal scanning laser microscopy respectively.ResultsA total of 34 DFI patients were recruited from which microbiota were analysed for 25 patients.P. aeruginosawas the most prevalent pathogen cultured and was one of the top 6 most prevalent and abundant species in the microbiota analysis. Phage APTC-PA18 was isolated from DFIs, belonged to themyoviridaefamily and was strictly lytic. PA18 was stable between 4 and 70 degrees Celsius and between pH 3 and 11. Seven of eightP. aeruginosaclinical isolates were sensitive to APTC-PA18, and when APTC-PA18 was combined with ciprofloxacin against planktonic and biofilm ofP. aeruginosa, synergistic effects were observedin vitroand in DFI tissue samples.ConclusionPhage APTC-PA18, when combined with ciprofloxacin, has the ability to killP. aeruginosaclinical isolates bothin vitroandex vivoand is a promising treatment option forP. aeruginosainfections in DFUs.

show abstract

Section: Discussionmentioning

confidence: 99%

Bacteriophage in combination with ciprofloxacin againstPseudomonas aeruginosainfections in diabetic foot ulcer patients

Liu,

Goonetilleke,

Hon

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Titze et al (Titze and Krömker, 2020). utilized a phage mixture (7.4 × 10 9 PFU/mL) against S. aureus isolate 7142 with nuc genepositive, resulting in a reduction of bacterial colonies by 4.2 log 10 CFU/mL after 12 h. Liu et al (2022) isolated four distinct bacteriophages against drug-resistant S. aureus from various sources, namely nasal swabs, soil, and sheep feces: APTC-SA-2, APTC-SA-4, APTC-SA-12, and APTC-SA-13. Genome sequencing revealed that none of these bacteriophages contained virulence or antibiotic resistance genes.…”

Section: In Vitro Experiments Of Phage Therapy Against Drug-resistant...mentioning

confidence: 99%

Bacteriophage therapy for drug-resistant Staphylococcus aureus infections

Liu,

Wang,

Zhou

et al. 2024

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Drug-resistant Staphylococcus aureus stands as a prominent pathogen in nosocomial and community-acquired infections, capable of inciting various infections at different sites in patients. This includes Staphylococcus aureus bacteremia (SaB), which exhibits a severe infection frequently associated with significant mortality rate of approximately 25%. In the absence of better alternative therapies, antibiotics is still the main approach for treating infections. However, excessive use of antibiotics has, in turn, led to an increase in antimicrobial resistance. Hence, it is imperative that new strategies are developed to control drug-resistant S. aureus infections. Bacteriophages are viruses with the ability to infect bacteria. Bacteriophages, were used to treat bacterial infections before the advent of antibiotics, but were subsequently replaced by antibiotics due to limited theoretical understanding and inefficient preparation processes at the time. Recently, phages have attracted the attention of many researchers again because of the serious problem of antibiotic resistance. This article provides a comprehensive overview of phage biology, animal models, diverse clinical case treatments, and clinical trials in the context of drug-resistant S. aureus phage therapy. It also assesses the strengths and limitations of phage therapy and outlines the future prospects and research directions. This review is expected to offer valuable insights for researchers engaged in phage-based treatments for drug-resistant S. aureus infections.

show abstract

“…Multiple studies have shown that isolated phages from different environmental sources exhibit high specificity towards specific bacterial strains, particularly against multidrug S. aureus [114][115][116][117][118]. Phage cocktails, composed of lytic bacteriophages individually targeting specific pathogenic strains, have shown significant results, influencing their administration [119][120][121][122][123]. A recent study demonstrated the efficacy of a potent phage cocktail named StaphLyse.…”

Section: Exploring the Potential Of Phage Therapy In Modern Medicinementioning

confidence: 99%

Emerging strategies and therapeutic innovations for combating drug resistance in Staphylococcus aureus strains: A comprehensive review

Gopikrishnan,

Haryini,

2024

J Basic Microbiol

View full text Add to dashboard Cite

In recent years, antibiotic therapy has encountered significant challenges due to the rapid emergence of multidrug resistance among bacteria responsible for life‐threatening illnesses, creating uncertainty about the future management of infectious diseases. The escalation of antimicrobial resistance in the post‐COVID era compared to the pre‐COVID era has raised global concern. The prevalence of nosocomial‐related infections, especially outbreaks of drug‐resistant strains of Staphylococcus aureus, have been reported worldwide, with India being a notable hotspot for such occurrences. Various virulence factors and mutations characterize nosocomial infections involving S. aureus. The lack of proper alternative treatments leading to increased drug resistance emphasizes the need to investigate and examine recent research to combat future pandemics. In the current genomics era, the application of advanced technologies such as next‐generation sequencing (NGS), machine learning (ML), and quantum computing (QC) for genomic analysis and resistance prediction has significantly increased the pace of diagnosing drug‐resistant pathogens and insights into genetic intricacies. Despite prompt diagnosis, the elimination of drug‐resistant infections remains unattainable in the absence of effective alternative therapies. Researchers are exploring various alternative therapeutic approaches, including phage therapy, antimicrobial peptides, photodynamic therapy, vaccines, host‐directed therapies, and more. The proposed review mainly focuses on the resistance journey of S. aureus over the past decade, detailing its resistance mechanisms, prevalence in the subcontinent, innovations in rapid diagnosis of the drug‐resistant strains, including the applicants of NGS and ML application along with QC, it helps to design alternative novel therapeutics approaches against S. aureus infection.

show abstract

APTC-C-SA01: A Novel Bacteriophage Cocktail Targeting Staphylococcus aureus and MRSA Biofilms

Cited by 13 publications

References 50 publications

Bacteriophage in combination with ciprofloxacin againstPseudomonas aeruginosainfections in diabetic foot ulcer patients

Bacteriophage in combination with ciprofloxacin againstPseudomonas aeruginosainfections in diabetic foot ulcer patients

Bacteriophage therapy for drug-resistant Staphylococcus aureus infections

Emerging strategies and therapeutic innovations for combating drug resistance in Staphylococcus aureus strains: A comprehensive review

Contact Info

Product

Resources

About