Aqueous and enzyme-extracted phenolic compounds from brewers' spent grain (BSG): Assessment of their antioxidant potential

Crowley, D. J.; O’Callaghan, Yvonne C.; McCarthy, Aoife L.; Connolly, Alan; Fitzgerald, Richard J.; O’Brien, Nora M.

doi:10.1111/jfbc.12370

Cited by 13 publications

(13 citation statements)

References 39 publications

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“…However, in the same study one of the casein hydrolysates showed protective effects against H 2 O 2 ‐induced DNA damage in U937 cells as assessed using the comet assay. Crowley et al () reported that phenolic compounds extracted from brewer's spent grain significantly decreased H 2 O 2 ‐induced DNA damage in U937 cells. While these phenolic extracts also showed some protection against H 2 O 2 ‐induced reduction in GSH concentration and reduced SOD and CAT activities in HepG2 cells, the antioxidant activity of the fractions was lower in these assays compared to the comet assay.…”

Section: Discussionmentioning

confidence: 99%

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Bioactivity of bovine lung hydrolysates prepared using papain, pepsin, and Alcalase

O’Sullivan

Lafarga

Hayes³

et al. 2017

J Food Biochem

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Bovine lung tissue was hydrolyzed using three different proteases, papain, pepsin, or Alcalase, to generate hydrolysates. Hydrolysates showed little antioxidant activity in noncellular or cellular assays. The anti‐inflammatory activity of hydrolysates was assessed in RAW264.7 macrophages and Jurkat T cells. Treatment with the Alcalase hydrolysate significantly decreased the production of the pro‐inflammatory cytokines interleukin (IL)‐6 and IL‐1β in a dose dependent manner in RAW264.7 cells. Nitric oxide production also significantly decreased following treatment with this hydrolysate. The papain hydrolysate decreased IL‐6, IL‐1β, and NO production in RAW264.7 cells and IL‐2 production in Jurkat T cells. However, the decrease was likely due to cytotoxicity of this hydrolysate toward these cell lines. The pepsin hydrolysate had no effect on cytokine production in RAW264.7 cells and only slightly decreased IL‐2 production in Jurkat T cells. Practical applications Meat production generates large amounts of protein rich co‐products, which are often discarded as waste or sold as low value animal feeds. The enzymatic hydrolysis of these protein co‐products may produce hydrolysates that are capable of reducing oxidation or inflammation. Novel hydrolysates were generated from the hydrolysis of bovine lung and results from this study indicate that the hydrolysis of bovine lung using the commercially available protease Alcalase may have potential as an anti‐inflammatory agent.

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Section: Discussionmentioning

confidence: 99%

“…Following on from these studies, oxidative DNA damage was induced in cells using H 2 O 2 at a concentration of 80 μmol L −1 for 30 min. Previously published studies using the comet assay have employed similar concentrations of H 2 O 2 with U937 cells (Crowley et al, ; O'Sullivan, Lafarga, Hayes, & O'Brien, ).…”

Section: Methodsmentioning

confidence: 99%

Bioactivity of bovine lung hydrolysates prepared using papain, pepsin, and Alcalase

O’Sullivan

Lafarga

Hayes³

et al. 2017

J Food Biochem

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“…Antimicrobial activities (Barbosa-Pereira et al, 2014;Ibarruri et al, 2019;Kumari et al, 2019;Socaci et al, 2018) Antibacterial against gram-positive and negative Antifungal 3. DNA-protective and antimutagenic activities (Crowley et al, 2017;McCarthy et al, 2012;Socaci et al, 2018) 4. Immunomodulatory effects (Kumari et al, 2019;McCarthy et al, 2013) Protein and isolate 1.…”

Section: Lipid Peroxidationmentioning

confidence: 99%

Brewers’ spent grain in food systems: Processing and final products quality as a function of fiber modification treatment

Naibaho

Korzeniowska

2021

Journal of Food Science

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The nutritional properties of brewers' spent grain (BSG) have been widely studied, considering its potential as a healthy food ingredient. Because of its fiber composition (amount and ratio), however, adding BSG into the food matrix to bring about changes in physical properties has been believed to impact negatively on the acceptability of the final products' properties, particularly color and texture. Fiber modification can enhance the quality of fiber and can be applied to BSG. Although it appears challenging, modifying fiber composition requires further study, particularly if the acceptability of the final products is to be improved. Furthermore, the level of fiber degradation during the modification treatment needs to be examined to meet the increased demand for BSG in final food products. This concise synthesis provides a new perspective for increasing the use of BSG as a food ingredient that is characterized by high nutrition and acceptability.

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“…This may be because the temperature of fermentation (25°C) was not the optimal temperature for the action of lignocellulosic enzymes. Thus, to increase the extraction of TPC a enzymatic hydrolysis may be performed (Crowley et al, 2017).…”

Section: Tablementioning

confidence: 99%

Simultaneous production of lignocellulolytic enzymes and extraction of antioxidant compounds by solid-state fermentation of agro-industrial wastes

Leite

Silva

Salgado

et al. 2019

Industrial Crops and Products

117

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In recent years, the interest to find alternative extraction methods has increased. Extraction of natural phenolics by enzymes produced during solid-state fermentation (SSF) is a useful and novel technique environmentally friendly. On the other hand, agro-industrial wastes are an excellent source of natural antioxidants as phenolics. The aim of the work was to evaluate the increase of antioxidant compounds extraction from agro-industrial wastes after SSF and to relate it to the production of lignocellulolytic enzymes. Several filamentous fungi were evaluated as candidates to increase the extraction of antioxidant compounds by solid-state fermentation of wineries, olive mill and brewery wastes. Fermented and unfermented agro-industrial wastes were extracted with water and lignocellulolytic enzymes, total phenolic compounds and antioxidant activity were determined in the extract. The maximum xylanase and cellulase activities were achieved by A. ibericus strains using brewer´s spent grain (BSG) as substrate and ranged from 300 to 313 U xylanase/g and 51-62 U cellulase/g. The best producer of β-glucosidase was A. niger CECT2088 using BSG as substrate (94 ± 4 U/g). The results of extraction of phenolic compounds revealed a higher extraction by SSF in olive mill wastes followed by winery wastes. In the same form, the maximum increase of antioxidant activity was achieved by SSF of exhausted olive pomace by Rhizopus oryzae MUM 10.260, increasing 12.9-fold in relation to unfermented waste. In addition, the production of enzymes, the extraction of phenolic compounds and the increase of antioxidant activity were related by principal component analysis. The first component grouped the BSG along with maximum enzymes production, the second component related positively the production of enzymes and the extraction of phenolic compounds and the increase of antioxidant activity. SSF proved to be an innovative environmentally friendly process that can improve the extraction of antioxidant compounds and simultaneously to produce lignocellulolytic enzymes from different agro-industrial wastes.

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Aqueous and enzyme-extracted phenolic compounds from brewers' spent grain (BSG): Assessment of their antioxidant potential

Cited by 13 publications

References 39 publications

Bioactivity of bovine lung hydrolysates prepared using papain, pepsin, and Alcalase

Bioactivity of bovine lung hydrolysates prepared using papain, pepsin, and Alcalase

Brewers’ spent grain in food systems: Processing and final products quality as a function of fiber modification treatment

Simultaneous production of lignocellulolytic enzymes and extraction of antioxidant compounds by solid-state fermentation of agro-industrial wastes

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