Aqueous and Ethanolic Extracts of Boswellia serrata Protect Against Focal Cerebral Ischemia and Reperfusion Injury in Rats

Abstract

Oxidative stress and cell apoptosis play major roles in neuronal injury after ischemia-reperfusion (I-R) injury. Boswellia serrata is a medicinal plant with antioxidant properties. Acetyl-11-keto-β-boswellic acid (AKBA) is an active triterpenoid compound from B. serrate. In the current study, the neuroprotective effects of aqueous and ethanolic extracts of B. serrata (named ABS and EBS, respectively) and AKBA were investigated against middle cerebral artery occlusion-induced cerebral I-R injury in rats. ABS and EBS with doses of 125, 250 and 500 and AKBA with a dose of 50 mg/kg were administered (intraperitoneally) just after middle cerebral artery occlusion induction for 30 min and reperfusion for 24 h. HPLC analysis suggested that ABS and EBS had AKBA of 8.8% and 9.5% (w/w), respectively. B. serrata and AKBA significantly improved neurological deficit and reduced brain infarction, neuronal cell loss and apoptosis and also attenuated lipid peroxidation while increasing glutathione content and superoxide dismutase activity in the cerebral cortex following a stroke. Apoptosis suppression was found to be mediated through regulating caspase-3 and bax/bcl-2 expressions. In conclusion, our results demonstrated that B. serrata and AKBA attenuate oxidative damage and inhibit cell apoptosis, subsequently protecting cerebral I-R injury in rats. Copyright © 2016 John Wiley & Sons, Ltd.

Modulatory effects of curcumin on heat shock proteins in cancer: A promising therapeutic approach

“…In terms of herbal medicines, increasing demand for medicinal plants and related compounds has been spurred by their global use for the treatment of a myriad of diseases. 16 bright orange yellow in color. It is also known as diferuloylmethane.…”

“…In recent times, the belief that natural medicines having fewer side effects than synthetic drugs have become very popular and has led to a huge increase in phytopharmaceutical usage throughout the world. In terms of herbal medicines, increasing demand for medicinal plants and related compounds has been spurred by their global use for the treatment of a myriad of diseases …”

<p>Antioxidant and toxicity studies of biosynthesized cerium oxide nanoparticles in rats</p>

“…The levels of MDA, as the final product of lipid peroxidation process, were determined using thiobarbituric acid (TBA), as the reagent. 23,24 Briefly, 100 μL of serum was mixed with 2 mL of TCA-TBA-HCl reagent (15% trichloroacetic acid, 0.67% TBA, and 0.25 N HCl) and heated for 45 minutes in a boiling…”

“…The amount of MDA produced was calculated in μmol/L. 23 Determination of sOD activity SOD activity in serum was evaluated using a spectrophotometric method. 25 Briefly, the sample was incubated in a solution containing Tris-HCl buffer (pH 8.2, 50 mM) and EDTA (1 mM).…”

Role of Plant Extracts in Treatment of Cerebral Ischemia

“…In another study, it has been showed that treatment with hydroalcoholic extract of saffron improves spatial learning and memory in rats with cerebral ischemia through carotid artery ligation Boswellia serrata can be consider as a useful plant to treat cerebral ischemia due to oral administration of aqueous and ethanolic extracts obtained from Boswellia serrata lead to reduction of neurological deficit and brain infarction [23]. In addition, this extract has antioxidant and anti-apoptotic effect through reduction of lipid peroxidation and increase of glutathione level and superoxide dismutase activity as well as reduction of caspase-3 activity, down regulation of bax and up regulation of bcl-2 in rats with middle cerebral artery occlusion-induced cerebral ischemia-reperfusion injury [24]. In a study effect of ethanolic extract prepared from Embelia.…”