Aqueous IR Bagendit rice leaf extract decreases reticulocyte count in lead-exposed rats

Santosa, Budi; Sunoko, Henna Rya; Sukeksi, Andri

doi:10.18051/univmed.2018.v37.57-64

Cited by 3 publications

(4 citation statements)

References 14 publications

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“…20 Protein metallothionein in (Oryza sativa L) IR-Bagendit rice leaves has a sulfhydryl group and can bind PB acetate; thus, this protein metallothionein is stable for the detoxification process. 21,22 This study has found that the provision of IR-Bagendit rice (Oryza sativa L) leaf infusion of 0.2 ml/day, 0.4 ml/day, and 0.8 ml/day can successively decrease necrosis cell damage up to 75% in P1, 90% in P2, and 100% in P3. These numbers show that the intervention groups have significantly different improvements from the positive control group.…”

Section: Necrosis Normal Cellsmentioning

confidence: 96%

“…This protein is rich in sulfhydryl groups that could covalently bind heavy metals (Pb). 12,13 The content of metallothionein proteins in IR Bagendit rice leaves from various locations in Northern Java has been investigated, and the investigation has revealed that R Bagendit rice from Blora has the highest content. 14 The metallothionein gene is located on chromosome 3 of IR Bagendit rice leaves (Oryza Sativa) and functions as stress-inducible proteins in drought conditions as well as soil and water-containing metals.…”

Section: Introductionmentioning

confidence: 99%

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The effects of IR-Bagendit rice leaf infusions from Blora on renal tubular degeneration and necrosis

Santosa

Arif

Mukaromah

2022

J. Teknol. Laboratorium

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Plumbum exposure still becomes a health problem in Indonesia. Plumbum (Pb) is widely used in various industries; thus, it can cause health problems, including impaired kidney functions. The accumulation of Pb rise to degeneration and necrosis of renal tubule cells. Therefore, preventive efforts are necessary to inhibit renal function deterioration. Infused extract of IR Bagendit rice leaf from Blora contains protein metallothionein which could bind PB covalently. This study aims to determine the supplementation of IR Bagendit rice leaf infusion to inhibit degeneration and necrosis of renal tubular cells exposed to Pb. The experimental research method was conducted using Wistar albino rats divided into five groups: negative control group, positive group, and treatment groups 1, 2, and 3. Each group consisted of six rats. The negative control group only received a placebo, and the positive group only received Pb acetate 60 mg/kg BW/day. Meanwhile, treatment groups 1, 2, and 3 received 0.2 ml, 0.4 ml, and 0.8 ml IR Bagendit rice leaf infusions successively and Pb acetate 60 mg/kg BW/day. The experiment was conducted for 60 days. Histological preparations of tubular cells were colored using hematoxylin-eosin. Normal cell differences, degeneration, and necrosis of each group were tested using Kruskal Wallis and Mann Whitney tests.This study has revealed that the average normal cells in the positive control group are lower than those in the negative control group and treatment groups 1, 2, and 3. This difference is insignificant with the p = 0.477. Moreover, treatment groups 1, 2, and 3 show decreased cell degeneration and necrosis with significant differences of p = 0.000 and 0.001. IR Bagendit rice leaf infusions could inhibit degeneration and necrosis of renal tubular cells exposed to Pb.

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Section: Necrosis Normal Cellsmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

The effects of IR-Bagendit rice leaf infusions from Blora on renal tubular degeneration and necrosis

Santosa

Arif

Mukaromah

2022

J. Teknol. Laboratorium

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“…8,9 Anemia terjadi karena sintesis heme tidak terbentuk. 10,11 Pada proses sintesis heme, timbal menghambat enzim δ ALAD (amino levulinic acid dehydratase) sehinga mengakibatkan terhentinya δ ALA (δ amino levulinic acid) menjadi porphobilinogen (PBG). 12 Hal ini dapat mengakibatkan peningkatan kadar δ ALA dalam darah dan urin.…”

Section: Pendahuluanunclassified

Sel Retikulosit Pada Paparan Timbal Pekerja Bengkel Cat Mobil Di Kampung Ligu Semarang

Santosa

2022

eum

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Paparan timbal pada pekerja bengkel pengecatan mobil di kampung Ligu Semarang dapat menimbulkan anemia. Sel retikulosit akan meningkat pada penderita anemia. Tujuan penelitian ini adalah untuk mengetahui sel retikulosit akibat paparan timbal pada pekerja bengkel pengecatan mobil di kampung Ligu Semarang. Cross sectional study digunakan untuk penelitian ini. Seluruh pekerja pengecatan mobil di kampung Ligu Semarang digunakan sebagai populasi, sampel sebanyak 46 diambil secara acak dan memenuhi kriteria inklusi dan eksklusi. Spesimen darah diambil pada seluruah sampel untuk diperiksa jumlah retikulosit dan kadar timbal. Jumlah retikulosit diperiksa dengan metoda langsung dan tak langsung menggunakan pengecatan BCB. Kadar timbal diperiksan menggunakan AAS. Jumlah retikulosit dan timbal dilihat rerata, di uji r person, dan regresi linier. Hasil Penelitian menunjukkan rerata retikulosit 1,60±0,47, rerata timbal 43,25±4,99. Hasil uji statistik diperoleh r 0,75, p 0,00 yang artinya ada korelasi kuat dan siknifikan kadar timbal dengan paparan jumlah sel reikulosit. Uji regresi linier diperoleh p=0,00 dengan rumus regresi: timbal=7,98 retikulosit+30,64. Bila timbal naik 1 µg/dl maka diikuti kenaikan jumlah retikulosit sebesar 7,98%. Simpulan terdapat korelasi kuat dan siknifikan antara peningkatan kadar timbal dengan penambahan jumlah retikulosit dengan nilai prediksi timbal=7,98 retikulosit+30,64. Kata kunci : retikulosit. Timbal, pekerja bengkel cat mobil.

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“…IR Bagendit rice leaves-infused water is rich in the metallothionein protein (Santosa et al, 2018;Santosa et al, 2020) and has many sulfhydryl groups that function in the HG detoxication mechanism. A large number of sulfhydryl groups in the metallothionein protein affects the protein's ability to bind Hg.…”

Section: Introductionmentioning

confidence: 99%

Blood Profile of Rattus Nurvegicus Exposed to HgCl2 and Received Combination of IR Bagendit Rice Leaves-Infused Water and Young Coconut Water

Santosa

2022

Med.Lab.Tech.J.

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Mercury chloride (HgCl2) is widely used in industries; in fact, this chemical substance is deadly for health and causes various health problems, such as liver damage, kidney damage, and hematopoietic disorders, especially in erythropoiesis. This study aims to determine the blood profile in Rattus nurvegicus exposed to HgCl2 by combining IR Bagendit rice leaves-infused water and young coconut water. This study employed an experimental method to examine the positive control group exposed to HgCl2 and the negative control group receiving a placebo. Meanwhile, the treatment groups received a combination of IR Bagendit rice leaves-infused water in stratified compositions and young coconut water. The hematologic profile was examined using a hematologic analyzer, while the reticulocyte count was examined using wet and dry methods. The mean value of each group was tested using ANOVA. This study has successively obtained average levels of Hb (C+ 12,0;C- 14,2;T1 12,5;T2 12,9;T3 12,8), the erythrocyte count (C+ 6,52;C- 7,80;T1 1,44;T2 1,07;T3 7,32), levels of Ht (C+ 35,5;C- 42,9;T1 37,8;T2 37,9;T3 38,2), MCV (C+ 73,7;C- 79,9;T1 80,4;T2 78,7;T3 79,6), MCH (C+ 27,3;C- 29,9;T1 29,5;T2 28,5;T3 29,1), and MCHC (C+ 31,1;C- 34,2;T1 31,9;T2 33,3;T3 32,1), and the reticulocyte count (C+ 2,0;C- 1,2;T1 1,3;T2 1,1;T3 1,3). The significant differences in the groups are found in levels of Hb, Ht, MCV, and MCHC (p-value 0,00; 0,00; 0,03; 0,01). This study concludes that combining IR Bagendit rice leaves-infused water and coconut water could prevent blood profile exposure to HgCl2. Suggestions for further research to increase the intervention time and measure oxygen levels.

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Aqueous IR Bagendit rice leaf extract decreases reticulocyte count in lead-exposed rats

Cited by 3 publications

References 14 publications

The effects of IR-Bagendit rice leaf infusions from Blora on renal tubular degeneration and necrosis

The effects of IR-Bagendit rice leaf infusions from Blora on renal tubular degeneration and necrosis

Sel Retikulosit Pada Paparan Timbal Pekerja Bengkel Cat Mobil Di Kampung Ligu Semarang

Blood Profile of Rattus Nurvegicus Exposed to HgCl2 and Received Combination of IR Bagendit Rice Leaves-Infused Water and Young Coconut Water

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