Aqueous seeded RAFT polymerization for the preparation of self-assemblies containing nucleobase analogues

Abstract

Self-assemblies containing the nucleobase analogue 2,6-diacylaminopyridine (DAP) have been successfully prepared for the first time by aqueous seeded RAFT polymerization in high concentrations. For this purpose, a diblock copolymer containing...

Polymerization‐induced self‐assembly for drug delivery: A critical appraisal

“…Post‐PISA loading methods are in essence analogous to those explored for conventionally assembled NPs: Physical encapsulation of hydrophobic cargo can be achieved by dissolving small amounts of cargo in the dispersion medium 124 or via latex swelling with a suitable organic solvent 18,123 . Crosslinking is recommended for improved retention of the NP morphology during the swelling/loading procedure 124,125,138 . For vesicles, hydrophilic cargo can be encapsulated in the aqueous lumen through a different approach: For instance, Mable et al 100 took inspiration from cell transfection procedures to incorporate EGFP plasmid DNA in PISA‐derived, pH‐responsive framboidal polymersomes via electroporation.…”

“…Moreover, post-PISA functionalization proves beneficial in creating loaded PISA-NPs with dual functionality, like combining active targeting and cargo delivery. 101 Post-PISA loading methods are in essence analogous to those explored for conventionally assembled NPs: Physical encapsulation of hydrophobic cargo can be achieved by dissolving small amounts of cargo in the dispersion medium 124 or via latex swelling with a suitable organic solvent. 18,123 Crosslinking is recommended for improved retention of the NP morphology during the swelling/loading procedure.…”

“…18,123 Crosslinking is recommended for improved retention of the NP morphology during the swelling/loading procedure. 124,125,138 For vesicles, hydrophilic cargo can be encapsulated in the aqueous lumen through a different approach: For instance, Mable et al 100 took inspiration from cell transfection procedures to incorporate EGFP plasmid DNA in PISA-derived, pH-responsive framboidal polymersomes via electroporation.…”

“…229 Wavelength-orthogonality may be exploited for successive photo-PISA and crosslinking. 227,229 Given the complexity of incorporating covalent crosslinking in PISA-NPs as well as its frequent need for biologically unfavorable chemicals, non-covalent crosslinking relying on hydrogen bonding 124 or electrostatic interactions 230 may be considered. For example, Abad et al 124 incorporated repeat units bearing the nucleobase analogue 2,6-diacylaminopyridine (DAP) between the PEG-based shell and the hydrophobic PHPMAbased micellar core.…”

“…227,229 Given the complexity of incorporating covalent crosslinking in PISA-NPs as well as its frequent need for biologically unfavorable chemicals, non-covalent crosslinking relying on hydrogen bonding 124 or electrostatic interactions 230 may be considered. For example, Abad et al 124 incorporated repeat units bearing the nucleobase analogue 2,6-diacylaminopyridine (DAP) between the PEG-based shell and the hydrophobic PHPMAbased micellar core. The DAP units were employed for non-covalent crosslinking with a molecule containing four thymine units, driven by triple hydrogen bonding per unit.…”