Aqueous shift reagents for high-resolution cation NMR. V. Thermodynamics of interaction of DyTTHA3− with Na+, K+, Mg 2+ , and Ca 2+

Chu, Simon C. K.; Qiu, Howard; Springer, Charles S.; Wishnia, Arnold

doi:10.1016/0022-2364(90)90006-u

Cited by 9 publications

(4 citation statements)

References 24 publications

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“…The facts that, of the simple inorganic cations present in KH buffer solution, Ca" has the greatest affinity for Dy?THA3-(Ref. 19) and that TmDOTPS-is particularly sensitive to Ca2+ (Ref. 49) hint strongly at Ca2+ playing the role of this competitor; at least partly, if not completely.…”

Section: Ca2+ Competition For Srsmentioning

confidence: 99%

Aqueous shift reagents for high‐resolution cation NMR. VI. Titration curves for in vivo²³Na and ¹H₂O MRS obtained from rat blood

et al. 1993

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Frequency shift/concentration calibration curves applicable to the use of shift reagents (SRs) for in vivo 23Na MRS studies can be obtained from experiments with whole blood. Here, they are reported for titrations of rat blood with the SRs DyTTHA3- and TmDOTP5-. There are a number of considerations that must be made in order to derive accurate calibration curves from the experimental data. These include the effects of bulk magnetic susceptibility (BMS, since the SRs are paramagnetic), the effects of water flux (since addition of the SR stock solution to blood renders the plasma hyperosmotic), and the consequences of restricted distribution of the SR anion in the erythrocyte suspension. We give in some detail the BMS shift theory that obtains in this case and show also how it applies to excised perfused organ as well as in vivo studies. Also, we report significant effects of adjuvant Ca2+ additions in the TmDOTP5- titrations. These are very important to the successful use of this SR in vivo. Finally, our considerations of BMS lead naturally to an understanding of its manifestations in the shifts of the 1H2O resonance frequencies of cell suspensions and tissues induced by SRs. Since these are being increasingly reported, and often misinterpreted, we devote an experiment and some discussion to this subject. We show that this phenomenon cannot be used to quantitatively discriminate intra- and extracellular 1H2O signals.

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Section: Ca2+ Competition For Srsmentioning

confidence: 99%

Aqueous shift reagents for high‐resolution cation NMR. VI. Titration curves for in vivo²³Na and ¹H₂O MRS obtained from rat blood

et al. 1993

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“…This would increase the fractional binding of cations to the shift reagent complex, thus increasing the downfield shift. 12 The decrease in shift in the ischemic limb ( Fig. 5(a)) parallels the washout seen in the well-perfused limb when shift reagent infusion is halted ( Fig.…”

Section: The Fraction Of the Sodium Signal Affected By Shift Reagent mentioning

confidence: 66%

Letter to the editor

Blum

Osbakken

1991

NMR in Biomedicine

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“…Dy(TTHA) 3Ϫ presents some advantages: it does not carry Na ϩ counterion to the buffer, it is fairly insensitive to pH variations (32), and the linewidth of the external sodium resonance is not enlarged; however, its shifting performance is very low. Under our experimental conditions, a shift was observed only with 25 mM external sodium concentration when this shift reagent was 10 mM.…”

Section: Discussionmentioning

confidence: 99%

“…Second the linewidth of the external Na ϩ resonance is very large in the presence of this shift reagent (⌬ 1/2 ϭ 110 Hz); Castle et al (7) observed the same phenomenon in E. coli (⌬ 1/2 ϭ 90 Hz). Finally, Dy(PPP i ) 2 7Ϫ is very sensitive to the external environment such as pH and ion concentrations (32), consequently it was difficult to use this reagent when ionophores were added. Also, some authors showed that this reagent can be toxic for the cells: First large interactions of PPP i with cellular cations (Ca 2ϩ , Mg 2ϩ ) disturbing metabolic functions were observed; as a consequence Ca 2ϩ infusions were necessary for in vivo studies in animals (34).…”

Section: Discussionmentioning

confidence: 99%

23Na NMR Study of Fibrobacter succinogenes S85: Comparison of Three Chemical Shift Reagents and Calculation of Sodium Concentration Using Ionophores

Delort

Gaudet

Forano

2002

Analytical Biochemistry

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Aqueous shift reagents for high-resolution cation NMR. V. Thermodynamics of interaction of DyTTHA3− with Na+, K+, Mg 2+ , and Ca 2+

Cited by 9 publications

References 24 publications

Aqueous shift reagents for high‐resolution cation NMR. VI. Titration curves for in vivo²³Na and ¹H₂O MRS obtained from rat blood

Aqueous shift reagents for high‐resolution cation NMR. VI. Titration curves for in vivo²³Na and ¹H₂O MRS obtained from rat blood

Letter to the editor

23Na NMR Study of Fibrobacter succinogenes S85: Comparison of Three Chemical Shift Reagents and Calculation of Sodium Concentration Using Ionophores

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Aqueous shift reagents for high-resolution cation NMR. V. Thermodynamics of interaction of DyTTHA3− with Na+, K+, Mg 2+ , and Ca 2+

Cited by 9 publications

References 24 publications

Aqueous shift reagents for high‐resolution cation NMR. VI. Titration curves for in vivo23Na and 1H2O MRS obtained from rat blood

Aqueous shift reagents for high‐resolution cation NMR. VI. Titration curves for in vivo23Na and 1H2O MRS obtained from rat blood

Letter to the editor

23Na NMR Study of Fibrobacter succinogenes S85: Comparison of Three Chemical Shift Reagents and Calculation of Sodium Concentration Using Ionophores

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Aqueous shift reagents for high‐resolution cation NMR. VI. Titration curves for in vivo²³Na and ¹H₂O MRS obtained from rat blood

Aqueous shift reagents for high‐resolution cation NMR. VI. Titration curves for in vivo²³Na and ¹H₂O MRS obtained from rat blood