Aqueous two-phase system: An alternative process for recovery of succinic acid from fermentation broth

Gu, Bo Hua; Zheng, Pu; Yan, Qiang; Liu, Wei

doi:10.1016/j.seppur.2014.09.034

Cited by 32 publications

(6 citation statements)

References 38 publications

(49 reference statements)

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“…However, the dissociated state α‐KG was mainly distributed in the lower phase, which was difficult to be extracted into the organic phase 27 . Neither Na 2 HPO 4 nor KH 2 PO 4 could form an ATPS with organic solvents, which is caused by the lower solubility than other salts, 38 and it was prone to saturation and precipitation during the extraction process. Generally, the higher the solubility of inorganic salt, the stronger its ability to compete for water molecules, promoting more water molecules to transfer from the organic phase to the inorganic salt phase to form ATPS.…”

Section: Resultsmentioning

confidence: 99%

Separation of α‐ketoglutaric acid by salting‐out extraction coupled with solar‐driven distillation

et al. 2022

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Alpha‐ketoglutaric acid (α‐KG), as an essential intermediate in biosynthesis and drug synthesis, has a broad application prospect. However, the lower product concentration and impurities in the α‐KG production make the downstream separation more complex and costly. In this study, α‐KG was separated from biotransformation broth by salting‐out extraction (SOE) combined with solar‐driven distillation. First, the aqueous two‐phase system (ATPS) consisting of acetone/(NH4)2SO4 was selected by comparison. The effects of acetone/(NH4)2SO4 concentration, temperature, α‐KG concentration, and pH on the distribution behavior of α‐KG in ATPS were investigated. Under the optimized conditions, higher extraction efficiency and purity of α‐KG were obtained in the actual biotransformation broth. In addition, solar evaporation was used to achieve preconcentration of the organic phase and salt recovery, significantly reducing energy consumption. The method is environmentally friendly and easy to operate, providing a new idea for separating low concentration products in biosynthesis.

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Section: Resultsmentioning

confidence: 99%

Separation of α‐ketoglutaric acid by salting‐out extraction coupled with solar‐driven distillation

et al. 2022

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“…Acetone and ammonium sulfate are added to the acid-broth mixture, and ultimately two phases are formed. These were found to be the best organic solvent/salt combination because their chemical properties allow for the fermentation impurities to settle in the heavy ammonium sulfate lower phase, and the succinic acid product to settle in the light acetone upper phase (Gu et al, 2014). Four more unit operations are needed to act upon the light phase to recover the succinic acid at 99.5% purity, including distillation, activated carbon decolorization, crystallization and tray drying.…”

Section: Additional Production Methods Investigatedmentioning

confidence: 99%

The design and techno economic analysis of a succinic acid production facility

Tomczyk

Öztürk²,

Wawrzyniak³

et al. 2022

Front. Sustain.

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Succinic acid is an industrially important commodity chemical that acts as a precursor to numerous other chemicals. This article explores an alternative biochemical route (as opposed to the traditional route from petroleum), by modeling a succinic acid production plant using Actinobacillus succinogenes, to convert the renewable sugars of corn stover feedstock. The model developed with SuperPro Designer v9.0 was used to evaluate the feasibility of this approach. This design is on par with the throughput of currentlarge scale manufacturers, by achieving an annual production rate of 11.5 US kilotons of succinic acid. The plant's economic standing is very attractive with a payback time of just under 5 years and return on investment of 20.4%. This article shows a facility design that maximizes profitability for a feasible and sustainable solution for bio-based succinic acid production.

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“…Currently, due to the use of renewable resources as fermentation substrates for the production of value‐added compounds , the manufacturing processes result in rather complex aqueous systems, wherefrom the products need to be separated . Thus, these technological approaches require the implementation of advanced downstream processing methods, which contribute to higher production costs .…”

Section: Aqueous Fermentation Systemsmentioning

confidence: 99%

“…Additionally, to facilitate greater yields and overcome inhibition phenomena or mass transfer issues, highly diluted systems are employed. For further processing, especially for but not limited to platform molecules serving as intermediates within different value chains of the chemical industry, it is crucial that the water is reduced to a minimum or even removed , . Likewise, logistic considerations also dictate the concentration of the products for efficient storage and transportation to end customers.…”

Section: Aqueous Fermentation Systemsmentioning

confidence: 99%

Fermentation Titer Optimization and Impact on Energy and Water Consumption during Downstream Processing

et al. 2018

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A common focus of fermentation process optimization is the product titer. Different strategies to boost fermentation titer target whole‐cell biocatalyst selection, process control, and medium composition. Working at higher product concentrations reduces the water that needs to be removed in the case of aqueous systems and, therefore, lowers the cost of downstream separation and purification. Different approaches to achieve higher titer in fermentation are examined. Energy and water consumption data collected from different Cargill fermentation plants, i.e., ethanol, lactic acid, and 2‐keto‐L‐gulonic acid, confirm that improvements in fermentation titer play a decisive role in downstream economics and environmental footprint.

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Aqueous two-phase system: An alternative process for recovery of succinic acid from fermentation broth

Cited by 32 publications

References 38 publications

Separation of α‐ketoglutaric acid by salting‐out extraction coupled with solar‐driven distillation

Separation of α‐ketoglutaric acid by salting‐out extraction coupled with solar‐driven distillation

The design and techno economic analysis of a succinic acid production facility

Fermentation Titer Optimization and Impact on Energy and Water Consumption during Downstream Processing

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