Aqueous two-phase systems containing urea: Influence of protein structure on protein partitioning

The development of recovery processes using aqueous two-phase systems (ATPS) has been limited by the extensive experimental work required to establish the optimal system parameters to ensure selective partitioning of the product of interest. Although using full factorial experiments has been demonstrated to be an effective strategy for the characterization of the partitioning behaviour of biological products in ATPS, this approach is characterized by its costly and time consuming nature. As an alternative, the use of a robotic-aided strategy has been proposed. However, the need for high cost equipment may limit the generic implementation of this strategy. Based on practical experience using ATPS, practical strategies for the predictive design of primary recovery processes using polymer-salt systems were derived. To evaluate the generic application of the proposed strategies, four experimental models (B-phycoerythrin, C-phycocyanin, double layered rotavirus-like particles and lutein) were selected. The application of these strategies resulted in the development of simplified recovery processes for the selected experimental models. The practical review presented is considered a relevant contribution that will provide general rules to facilitate the establishment of ATPS processes, particularly for new researchers in the field.

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Practical experiences from the development of aqueous two‐phase processes for the recovery of high value biological products

Benavides

Rito‐Palomares

2007

J of Chemical Tech & Biotech

142

122

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High throughput characterization of structural differences between closely related proteins in solution

Zaslavsky

Madeira

Breydo

et al. 2013

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

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Structural features important for differences in protein partitioning in aqueous dextran–polyethylene glycol two-phase systems of different ionic compositions

Ferreira

Fan

Mikheeva

et al. 2014

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

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Aqueous two-phase systems containing urea: Influence of protein structure on protein partitioning

Cited by 15 publications

References 37 publications

Practical experiences from the development of aqueous two‐phase processes for the recovery of high value biological products

Practical experiences from the development of aqueous two‐phase processes for the recovery of high value biological products

High throughput characterization of structural differences between closely related proteins in solution

Structural features important for differences in protein partitioning in aqueous dextran–polyethylene glycol two-phase systems of different ionic compositions

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