Aqueous Two‐Phase Systems for Gentle Separation of Bio‐Active Compounds Using Centrifugal Partition Chromatography

Schwienheer, C.; Merz, J.; Schembecker, Gerhard

doi:10.1002/cite.201450141

Cited by 1 publication

(5 citation statements)

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“…It is known from previous experiments that the rotational speed does not significantly influence the separation efficiency [30]. Thus, 750 rpm was the rotational speed in all cases [22,30]. The results are shown in Figure 5.…”

Section: Optimizing the Resolutionmentioning

confidence: 76%

“…Therefore, the resolution was measured for different phase retentions and volumetric flow rates of the mobile phase. It is known from previous experiments that the rotational speed does not significantly influence the separation efficiency [30]. Thus, 750 rpm was the rota-…”

Section: Optimizing the Resolutionmentioning

confidence: 98%

“…All in all, the redosing of the stationary phase as a newly developed mode of operation offers some benefits compared to different approaches, addressing the loss of the stationary phase over time. Sequential Centrifugal Chromatography or Continuous Centrifugal Extraction both circumvent the drawbacks in separation efficiency caused by bleeding but require high technical effort for implementation as well as complex operation [22,30,[38][39][40].…”

Section: Reference Experiments Without Redosingmentioning

confidence: 99%

“…As a biphasic system, the Arizona N system was chosen (1:1:1:1 volumetric amounts of water, ethyl acetate, n-heptane, and methanol) [28]. In previous publications, we conducted intensive research on the behavior and properties of different solvent systems of the Arizona series [22,29,30]. Therein, the Arizona N system has been studied most, including, among others, flow regimes and the optimization of the chamber design [17,22,23,30].…”

Section: Introductionmentioning

confidence: 99%

“…In previous publications, we conducted intensive research on the behavior and properties of different solvent systems of the Arizona series [22,29,30]. Therein, the Arizona N system has been studied most, including, among others, flow regimes and the optimization of the chamber design [17,22,23,30]. Another advantage of the Arizona N system is its straightforward handling, and the ratio between the upper and lower phase (0.7) is comparably similar [28].…”

Section: Introductionmentioning

confidence: 99%

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Optimizing Chromatographic Separation with Redosing: Effects on Separation Efficiency of a Model System in Centrifugal Partition Chromatography

Buthmann,

Hohlmann,

Neuwald

et al. 2024

Separations

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This study investigates and optimizes chromatographic separation in a Centrifugal Partition Chromatograph. Therefore, a model system is separated in a single-disc rotor. The occurring loss of the stationary phase lowers the separation efficiency over time. We introduced a new mode of operation, called the redosing of the stationary phase, to counteract this hydrodynamic phenomenon. Experiments with redosing at an optimized operating point demonstrate almost constant separation performance over 12 h, reducing solvent consumption by 45% and increasing chromatographic resolution by 37%. The improvement in retention by 69% contributes to this enhancement. Accordingly, reference experiments without redosing were conducted as a benchmark, highlighting the automated mode’s benefits, as mentioned.

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Section: Optimizing the Resolutionmentioning

confidence: 76%

Section: Optimizing the Resolutionmentioning

confidence: 98%