Arabidopsis IAR4 Modulates Primary Root Growth Under Salt Stress Through ROS-Mediated Modulation of Auxin Distribution

Fu, Yang; Yong, Yang; Chen, Shaoping; Ning, Nina; Hu, Honghong

doi:10.3389/fpls.2019.00522

Cited by 70 publications

(49 citation statements)

References 66 publications

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“…In 2-month-old wild-type birch, BpPP2C1 is mainly expressed in mature stems and highly expressed in roots ( Figure 3B ). Roots is the first organ of plant to receive the salt stress signal, which then affects the growth of the above-ground parts ( Fu et al, 2019 ). BpPP2C1 is a phosphate signal transmitter, and its abundant expression in roots is likely to be a great significance for plants to respond to salt stress.…”

Section: Discussionmentioning

confidence: 99%

Functional Study of BpPP2C1 Revealed Its Role in Salt Stress in Betula platyphylla

Xing

Zhang

et al. 2021

Front. Plant Sci.

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PP2C protein phosphatase family is one of the largest gene families in the plant genome. Many PP2C family members are involved in the regulation of abiotic stress. We found that BpPP2C1 gene has highly up-regulated in root under salt stress in Betula platyphylla. Thus, transgenic plants of Betula platyphylla with overexpression and knockout of BpPP2C1 gene were generated using a zygote transformation system. Under NaCl stress treatment, we measured the phenotypic traits of transgenic plants, chlorophyll-fluorescence parameters, peroxidase (POD) activity, superoxide dismutase (SOD) activity, and malondialdehyde (MDA) content. We found that BpPP2C1 overexpressed lines showed obvious salt tolerance, while BpPP2C1 knocked out plants were sensitive to salt stress. Transcriptome analysis identified significantly amount of differentially expressed genes associated with salt stress in BpPP2C1 transgenic lines, especially genes in abscisic acid signaling pathway, flavonoid biosynthetic pathway, oxidative stress and anion transport. Functional study of BpPP2C1 in Betula platyphylla revealed its role in salt stress.

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Section: Discussionmentioning

confidence: 99%

Functional Study of BpPP2C1 Revealed Its Role in Salt Stress in Betula platyphylla

Xing

Zhang

et al. 2021

Front. Plant Sci.

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“…The role of ROS as molecular regulators of plant signaling systems under the onset of salt stress action, which, when elevated, causes changes in phospholipids and Ca 2+ content and is accompanied by the production of abscisic acid, is one of the ways to form a cellular response to a stress factor [6,7].…”

Section: Introductionmentioning

confidence: 99%

Salt Stress-Induced Structural Changes Are Mitigated in Transgenic Tomato Plants Over-Expressing Superoxide Dismutase

Bogoutdinova

Лазарева

Chaban

et al. 2020

Biology

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Various abiotic stresses cause the appearance of reactive oxygen species (ROS) in plant cells, which seriously damage the cellular structures. The engineering of transgenic plants with higher production of ROS-scavenging enzyme in plant cells could protect the integrity of such a fine intracellular structure as the cytoskeleton and each cellular compartment. We analyzed the morphological changes in root tip cells caused by the application of iso-osmotic NaCl and Na2SO4 solutions to tomato plants harboring an introduced superoxide dismutase gene. To study the roots of tomato plants cultivar Belyi Naliv (WT) and FeSOD-transgenic line, we examined the distribution of ROS and enzyme-linked immunosorbent detection of α-tubulin. In addition, longitudinal sections of the root apexes were compared. Transmission electronic microscopy of atypical cytoskeleton structures was also performed. The differences in the microtubules cortical network between WT and transgenic plants without salt stress were detected. The differences were found in the cortical network of microtubules between WT and transgenic plants in the absence of salt stress. While an ordered microtubule network was revealed in the root cells of WT tomato, no such degree of ordering was detected in transgenic line cells. The signs of microtubule disorganization in root cells of WT plants were manifested under the NaCl treatment. On the contrary, the cytoskeleton structural organization in the transgenic line cells was more ordered. Similar changes, including the cortical microtubules disorganization, possibly associated with the formation of atypical tubulin polymers as a response to salt stress caused by Na2SO4 treatment, were also observed. Changes in cell size, due to both vacuolization and impaired cell expansion in columella zone and cap initials, were responsible for the root tip tissue modification.

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“…BC204 stimulated root growth under saline conditions ( Figure 4 ). A reduction in primary root length is one of the most common indicators of salt stress in plants [ 99 , 100 ]. Primary root length was significantly reduced in vitro on media containing 100 mM NaCl, however, a significant increase in primary root length was observed in these plants where BC204 was added to the medium in addition to the salt ( Figure 4 E).…”

Section: Discussionmentioning

confidence: 99%

The Application of a Commercially Available Citrus-Based Extract Mitigates Moderate NaCl-Stress in Arabidopsis thaliana Plants

Loubser

Hills

2020

Plants

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Aims: The aim of this study was to assess the effect of BC204 as a plant biostimulant on Arabidopsis thaliana plants under normal and NaCl-stressed conditions. Methods: For this study, ex vitro and in vitro growth experiments were conducted to assess the effect of both NaCl and BC204 on basic physiological parameters such as biomass, chlorophyll, proline, malondialdehyde, stomatal conductivity, Fv/Fm and the expression of four NaCl-responsive genes. Results: This study provides preliminary evidence that BC204 mitigates salt stress in Arabidopsis thaliana. BC204 treatment increased chlorophyll content, fresh and dry weights, whilst reducing proline, anthocyanin and malondialdehyde content in the presence of 10 dS·m−1 electroconductivity (EC) salt stress. Stomatal conductivity was also reduced by BC204 and NaCl in source leaves. In addition, BC204 had a significant effect on the expression of salinity-related genes, stimulating the expression of salinity-related genes RD29A and SOS1 independently of NaCl-stress. Conclusions: BC204 stimulated plant growth under normal growth conditions by increasing above-ground shoot tissue and root and shoot growth in vitro. BC204 also increased chlorophyll content while reducing stomatal conductivity. BC204 furthermore mitigated moderate to severe salt stress (10–20 dS·m−1) in A. thaliana. Under salt stress conditions, BC204 reduced the levels of proline, anthocyanin and malondialdehyde. The exact mechanism by which this occurs is unknown, but the results in this study suggest that BC204 may act as a priming agent, stimulating the expression of genes such as SOS1 and RD29A.

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Arabidopsis IAR4 Modulates Primary Root Growth Under Salt Stress Through ROS-Mediated Modulation of Auxin Distribution

Cited by 70 publications

References 66 publications

Functional Study of BpPP2C1 Revealed Its Role in Salt Stress in Betula platyphylla

Functional Study of BpPP2C1 Revealed Its Role in Salt Stress in Betula platyphylla

Salt Stress-Induced Structural Changes Are Mitigated in Transgenic Tomato Plants Over-Expressing Superoxide Dismutase

The Application of a Commercially Available Citrus-Based Extract Mitigates Moderate NaCl-Stress in Arabidopsis thaliana Plants

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