Arabidopsis myrosinases link the glucosinolate-myrosinase system and the cuticle

Ahuja, Ishita; Vos, Ric C. H. de; Rohloff, Jens; Stoopen, Geert; Halle, Kari Krizak; Ahmad, Shabbir; Hoang, Linh; Hall, Robert D.; Bones, Atle M.

doi:10.1038/srep38990

Cited by 19 publications

(16 citation statements)

References 60 publications

(89 reference statements)

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“…Considering the presence of GLSs in guard cells (Geng et al., 2016; Zhu and Assmann, 2017), how the GM system plays a role in guard cell functions (e.g., stomatal immunity) is an interesting question. Clearly, mutation of the TGG1 and/or TGG2 genes affected the guard cell size, stomatal aperture, and leaf metabolites, such as fatty acids, glucosinolates, and indole compounds (Ahuja et al., 2016). Another proteomic study of trichome and epidermal pavement cells did not identify the TGG1 protein in the samples (Wienkoop et al., 2004).…”

Section: Distinct Molecular and Biochemical Properties Of Myrosinasesmentioning

confidence: 99%

Chemodiversity of the Glucosinolate-Myrosinase System at the Single Cell Type Resolution

et al. 2019

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Glucosinolates (GLSs) are a well-defined group of specialized metabolites, and like any other plant specialized metabolites, their presence does not directly affect the plant survival in terms of growth and development. However, specialized metabolites are essential to combat environmental stresses, such as pathogens and herbivores. GLSs naturally occur in many pungent plants in the order of Brassicales. To date, more than 200 different GLS structures have been characterized and their distribution differs from species to species. GLSs co-exist with classical and atypical myrosinases, which can hydrolyze GLS into an unstable aglycone thiohydroximate-O-sulfonate, which rearranges to produce different degradation products. GLSs, myrosinases, myrosinase interacting proteins, and GLS degradation products constitute the GLS-myrosinase (GM) system (“mustard oil bomb”). This review discusses the cellular and subcellular organization of the GM system, its chemodiversity, and functions in different cell types. Although there are many studies on the functions of GLSs and/or myrosinases at the tissue and whole plant levels, very few studies have focused on different single cell types. Single cell type studies will help to reveal specific functions that are missed at the tissue and organismal level. This review aims to highlight (1) recent progress in cellular and subcellular compartmentation of GLSs, myrosinases, and myrosinase interacting proteins; (2) molecular and biochemical diversity of GLSs and myrosinases; and (3) myrosinase interaction with its interacting proteins, and how it regulates the degradation of GLSs and thus the biological functions (e.g., plant defense against pathogens). Future prospects may include targeted approaches for engineering/breeding of plants and crops in the cell type-specific manner toward enhanced plant defense and nutrition.

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Section: Distinct Molecular and Biochemical Properties Of Myrosinasesmentioning

confidence: 99%

Chemodiversity of the Glucosinolate-Myrosinase System at the Single Cell Type Resolution

et al. 2019

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“…The glucosinolates are a group of secondary metabolites involved in ABA-regulated stomatal opening [ 104 ] and floral development in drought conditions [ 105 ]. The tgg1 tgg2 mutant showed stomata with closed aperture in leaves resembling plants in the face of drought stress [ 106 ]. Thus, carbohydrate hydrolysis can also be involved in ICE1-regulated anther dehydration.…”

Section: Resultsmentioning

confidence: 99%

“…For SEM analysis, tissues were dissected under anatomical lens (SMZ-161-BLED, Motic, China) if needed, then immediately mounted on aluminum stubs for SEM. For leaf tissues, small pieces (d = 8 mm) of leaves from about 5-week-old plants were cut, fixed, dehydrated and coated as previously described [ 106 ]. These images were taken with scanning electron microscope TM3000 (TM3000 Tabletop Microscope, HITACHI, Japan).…”

Section: Methodsmentioning

confidence: 99%

INDUCER OF CBF EXPRESSION 1 is a male fertility regulator impacting anther dehydration in Arabidopsis

Wei

Liu

et al. 2018

PLoS Genet

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INDUCER OF CBF EXPRESSION 1 (ICE1) encodes a MYC-like basic helix-loop-helix (bHLH) transcription factor playing a critical role in plant responses to chilling and freezing stresses and leaf stomata development. However, no information connecting ICE1 and reproductive development has been reported. In this study, we show that ICE1 controls plant male fertility via impacting anther dehydration. The loss-of-function mutation in ICE1 gene in Arabidopsis caused anther indehiscence and decreased pollen viability as well as germination rate. Further analysis revealed that the anthers in the mutant of ICE1 (ice1-2) had the structure of stomium, though the epidermis did not shrink to dehisce. The anther indehiscence and influenced pollen viability as well as germination in ice1-2 were due to abnormal anther dehydration, for most of anthers dehisced with drought treatment and pollen grains from those dehydrated anthers had similar viability and germination rates compared with wild type. Accordingly, the sterility of ice1-2 could be rescued by ambient dehydration treatments. Likewise, the stomatal differentiation of ice1-2 anther epidermis was disrupted in a different manner compared with that in leaves. ICE1 specifically bound to MYC-recognition elements in the promoter of FAMA, a key regulator of guard cell differentiation, to activate FAMA expression. Transcriptome profiling in the anther tissues further exhibited ICE1-modulated genes associated with water transport and ion exchange in the anther. Together, this work reveals the key role of ICE1 in male fertility control and establishes a regulatory network mediated by ICE1 for stomata development and water movement in the anther.

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“…Myrosinases are involved in the plant defense system and work in conjunction with glucosinolates produced by Brassicaceae species against biotic diseases. The dual glucosinolate-myrosinase system acts against biotic defense by catalyzing the breakdown of the glucosinolates upon plant damage, thereby producing hydrolysis products, which are toxic to pathogens and herbivores, and acting as a plant defense system to certain microbial pathogens [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Identification of MAMP-Responsive Plasma Membrane-Associated Proteins in Arabidopsis thaliana Following Challenge with Different LPS Chemotypes from Xanthomonas campestris

2020

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Lipopolysaccharides (LPS) are recognized as microbe-associated molecular patterns (MAMPs) responsible for eliciting defense-related responses and while the effects have been well-documented in mammals, there is a lack of knowledge regarding the mechanism of perception in plant systems and recognized structural moieties within the macromolecular lipoglycan structure. Thus, identification of the LPS plasma membrane (PM) receptor(s)/receptor complex in Arabidopsis thaliana through proteomics will contribute to a deeper understanding of induced defense responses. As such, structurally characterized LPS chemotypes from Xanthomonas campestris pv. campestris (Xcc) wild-type 8004 (prototypical smooth-type LPS) and mutant 8530 (truncated core with no O–chain) strains were utilized to pre-treat A. thaliana plants. The associated proteomic response/changes within the PM were compared over a 24 h period using mass spectrometry-based methodologies following three variants of LPS-immobilized affinity chromatography. This resulted in the identification of proteins from several functional categories, but importantly, those involved in perception and defense. The distinct structural features between wild-type and mutant LPS are likely responsible for the differential changes to the proteome profiles, and many of the significant proteins were identified in response to the wild-type Xcc LPS where it is suggested that the core oligosaccharide and O-chain participate in recognition by receptor-like kinases (RLKs) in a multiprotein complex and, notably, varied from that of the mutant chemotype.

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Arabidopsis myrosinases link the glucosinolate-myrosinase system and the cuticle

Cited by 19 publications

References 60 publications

Chemodiversity of the Glucosinolate-Myrosinase System at the Single Cell Type Resolution

Chemodiversity of the Glucosinolate-Myrosinase System at the Single Cell Type Resolution

INDUCER OF CBF EXPRESSION 1 is a male fertility regulator impacting anther dehydration in Arabidopsis

Identification of MAMP-Responsive Plasma Membrane-Associated Proteins in Arabidopsis thaliana Following Challenge with Different LPS Chemotypes from Xanthomonas campestris

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