Arachidic Acid in Extender Improves Post‐thaw Parameters of Cryopreserved Nili‐Ravi Buffalo Bull Semen

Ejaz, Rabea; Ansari, .; Rakha, Bushra Allah; Ullah, Nemat; Husna, Asma Ul; Iqbal, Razia; Akhter, Shamim

doi:10.1111/rda.12239

Cited by 22 publications

(17 citation statements)

References 25 publications

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“…More recently, addition of 8% coconut oil as a source of lauric acid to egg yolk based-extender (79), 5 ng/ml α-linoleic acid to the BioXcell ® (80), and 20 ng/ml arachidonic acid to tris-citric acid extender (81) enhanced quality of sperm following cryopreservation. Additionally, Iodixanol is commonly used as a medium for density gradient centrifugation.…”

Section: Extender Developmentmentioning

confidence: 99%

Advances in Cryopreservation of Bull Sperm

et al. 2019

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Cryopreservation of semen and artificial insemination have an important, positive impact on cattle production, and product quality. Through the use of cryopreserved semen and artificial insemination, sperm from the best breeding bulls can be used to inseminate thousands of cows around the world. Although cryopreservation of bull sperm has advanced beyond that of other species, there are still major gaps in the knowledge and technology bases. Post-thaw viability of sperm is still low and differs significantly among the breeding bulls. These weaknesses are important because they are preventing advances both in fundamental science of mammalian gametes and reproductive biotechnology. Various extenders have been developed and supplemented with chemicals to reduce cryodamage or oxidative stress with varying levels of success. More detailed insights on sperm morphology and function have been uncovered through application of advanced tools in modern molecular and cell biology. This article provides a concise review of progress in the cryopreservation of bull sperm, advances in extender development, and frontiers using diverse techniques of the study of sperm viability. This scientific resource is important in animal biotechnology because with the advances in discovery of sperm fertility markers, there is an urgent need to improve post-thaw viability and fertility of sperm through enhanced cryopreservation for precision agriculture to produce food animals to ensure food security on the global scale.

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Section: Extender Developmentmentioning

confidence: 99%

Advances in Cryopreservation of Bull Sperm

et al. 2019

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“…Fatty acids such as arachidonic acid improved postthawed spermatozoa motility, membrane integrity, acrosome integrity, viability, and DNA of buffalo bull spermatozoa [7].…”

Section: Buffalomentioning

confidence: 93%

“…Oxidants, such as reactive oxygen species (ROS) which are produced physiologically in living cells during respiration as well as by abnormal or dead sperm and phagocytic cells of both the ejaculate and female reproductive tract, affect the quality of postthawed sperm in animals [7,8]. These ROS can inhibit sperm motility, capacitation, and acrosome reaction mediated by lipid peroxidation (LPO) of sperm membrane.…”

Section: Oxidative Stress (Os)mentioning

confidence: 99%

The Roles of Antioxidants and Fatty Acids in Sperm Cryopreservation

Yimer¹,

Kaka²,

Rosnina³

et al. 2016

Cryopreservation in Eukaryotes

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Despite research developments in the area of sperm storage, it has become inevitable to realize a marked reduction in the quality of fresh semen following cryopreservation. As a result, research has continued and will also continue in the future looking forward for a much better and improved methods of sperm cryopreservation along with better understanding of the physical and biochemical challenges that the sperm has to face to survive during freezing. Among the various attempts made to improve the cryopreservation process and subsequently result in superior quality of sperm after thawing include manipulating the composition of semen extenders by addition of exogenous products including antioxidants and fatty acids. While fatty acids are added to strengthening plasma membrane stability, Antioxidants are incorporated to compensate the reduction in the endogenous antioxidants level of seminal plasma due to dilution as well as to combat with the excess reactive oxygen species (ROS) production during freezing. In this chapter, the roles of antioxidants and fatty acids in mammalian sperm cryopreservation, both from endogenous and exogenous perspectives, will be discussed with reference to the latest research findings.

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“…Sperm chromatin integrity was assessed as practiced by Ejaz et al (2014). Air dried smears of semen samples were fixed in 96% ethanol-acetone (1:1) at 4°C for 30min, hydrolyzed with 4N HCl at 25°C for 10-30 min.…”

Section: Sperm Chromatin Integritymentioning

confidence: 99%

In vitro Supplementation of Linoleic Acid Improves Quality of Cryopreserved Buffalo Semen

Ejaz

Qadeer

Ansari

et al. 2020

IJAR

Self Cite

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Present study was designed to evaluate the effect of linoleic acid (LA) supplementation in extender on post thaw quality of cryopreserved buffalo semen. Semen was collected from three adult Nili Ravi buffalo bulls of same age with artificial vagina (42°C) for five weeks (replicates; N=30). Qualified semen ejaculates (>1mL volume, >60% motility, >0.5 billion/mL concentration) were diluted in tris-citric acid extender containing 0.0 (control), 5.0, 10.0 and 20.0ng mL-1 of LA and were cryopreserved using standard procedures. Sperm motility and plasma membrane integrity were improved plessthan0.05) in extender containing 10.0 ng mL-1 of LA compared to other treatments and control while number of acrosome intact live sperm, chromatin integrity and number of morphologically normal sperms remained the same. In conclusion, LA supplementation in extender at 10.0 ng mL-1 was found to be beneficial to improve post thaw quality of cryopreserved buffalo semen.

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Arachidic Acid in Extender Improves Post‐thaw Parameters of Cryopreserved Nili‐Ravi Buffalo Bull Semen

Cited by 22 publications

References 25 publications

Advances in Cryopreservation of Bull Sperm

Advances in Cryopreservation of Bull Sperm

The Roles of Antioxidants and Fatty Acids in Sperm Cryopreservation

In vitro Supplementation of Linoleic Acid Improves Quality of Cryopreserved Buffalo Semen

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