Archetype JC virus efficiently propagates in kidney‐derived cells stably expressing HIV‐1 Tat

Nukuzuma, Souichi; Kameoka, Masanori; Sugiura, Shigeki; Nakamichi, Kazuo; Nukuzuma, Chiyoko; Miyoshi, Isao; Takegami, Tsutomu

doi:10.1111/j.1348-0421.2009.00166.x

Cited by 12 publications

(30 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is consistent with previous results showing replication of JCPyV DNA initiated by SV40 TAg is better than that of BKPyV DNA (Mahon et al, 2009). Archetype JCPyV DNA can replicate but not efficiently produce virus in POJ cells and COS-7 cells overexpressing JCPyV and SV40 TAg, respectively (Daniel et al, 1996; Hara et al, 1998; Nukuzuma et al, 2009). The difference in progeny production of archetype JCPyV from COS-7 cells compared to 293TT cells likely has to do with the very high levels of SV40 TAg produced in 293TT cells.…”

Section: Discussionmentioning

confidence: 99%

“…Archetype JCPyV DNA replicates in COS-7, simian kidney cells expressing SV40 TAg, and COS-7 cells expressing HIV-1 Tat (Hara et al, 1998; Nukuzuma et al, 2009). However, these systems show weak progeny production, taking up to 32 days to produce JCPyV as detected by hemagglutination assay.…”

Section: Discussionmentioning

confidence: 99%

“…Archetype JCPyV DNA also replicates in COS-7 cells, monkey cells that constitutively express SV40 TAg, but infectious progeny are not produced (Hara et al, 1998). Additionally, it has recently been shown that archetype JCPyV propagates in COS-7 cells stably expressing HIV-1 Tat after 32 days in culture (Nukuzuma et al, 2009). HIV-1 Tat acts in coordination with the cellular protein Purα to stimulate viral DNA replication (Daniel et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Efficient propagation of archetype BK and JC polyomaviruses

Broekema

Imperiale

2012

Virology

View full text Add to dashboard Cite

BKPyV and JCPyV are closely related, ubiquitious human pathogens that cause disease in immunocompromised patients. The DNA sequence of the regulatory regions distinguishes two forms of these viruses, designated archetype and rearranged. Although cell culture systems exist for rearranged BKPyV and JCPyV, currently there is no robust cell culture system to study the archetype viruses. Large T antigen (TAg) is a virally encoded protein required to initiate viral DNA synthesis. Because archetype virus produces undetectable levels of TAg, we hypothesized that TAg overexpression would stimulate archetype virus replication. Efficient propagation of the archetype forms of BKPyV and JCPyV was observed in 293TT cells, human embryonic kidney cells overexpressing SV40 TAg. Importantly, the archetypal structure of the regulatory region was maintained during viral growth. Significant replication was not observed for Merkel cell, KI, or WU polyomaviruses. 293TT cells provide a means of propagating archetype BKPyV and JCPyV for detailed study.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Efficient propagation of archetype BK and JC polyomaviruses

Broekema

Imperiale

2012

Virology

View full text Add to dashboard Cite

show abstract

“…Archetype JCV, which normally cannot be efficiently propagated in cell culture, can replicate in cells expressing HIV tat (369,370). tat has also been shown to be secreted from infected cells and internalized by uninfected cells, affecting cellular function (137,138,203,491,531) and thereby abrogating the necessity of JCV and HIV coinfection for the molecular interaction between these viruses.…”

Section: Pml and Hiv/aidsmentioning

confidence: 99%

Molecular Biology, Epidemiology, and Pathogenesis of Progressive Multifocal Leukoencephalopathy, the JC Virus-Induced Demyelinating Disease of the Human Brain

et al. 2012

View full text Add to dashboard Cite

SUMMARY Progressive multifocal leukoencephalopathy (PML) is a debilitating and frequently fatal central nervous system (CNS) demyelinating disease caused by JC virus (JCV), for which there is currently no effective treatment. Lytic infection of oligodendrocytes in the brain leads to their eventual destruction and progressive demyelination, resulting in multiple foci of lesions in the white matter of the brain. Before the mid-1980s, PML was a relatively rare disease, reported to occur primarily in those with underlying neoplastic conditions affecting immune function and, more rarely, in allograft recipients receiving immunosuppressive drugs. However, with the onset of the AIDS pandemic, the incidence of PML has increased dramatically. Approximately 3 to 5% of HIV-infected individuals will develop PML, which is classified as an AIDS-defining illness. In addition, the recent advent of humanized monoclonal antibody therapy for the treatment of autoimmune inflammatory diseases such as multiple sclerosis (MS) and Crohn's disease has also led to an increased risk of PML as a side effect of immunotherapy. Thus, the study of JCV and the elucidation of the underlying causes of PML are important and active areas of research that may lead to new insights into immune function and host antiviral defense, as well as to potential new therapies.

show abstract

“…A previous report has shown that Tat protein enhances the expression of the late promoter-dependent reporter gene of JCV in glial cells significantly [Tada et al, 1990]. Recently, it has also been shown that archetypal and PML-type JCV propagates efficiently in kidney-derived cells stably expressing Tat protein [Nukuzuma et al, 2009b;Nukuzuma et al, 2010].…”

Section: Introductionmentioning

confidence: 96%

Exogenous human immunodeficiency virus‐1 protein, tat, enhances replication of JC virus efficiently in neuroblastoma cell lines

Nukuzuma¹,

Kameoka

Sugiura

et al. 2012

Journal of Medical Virology

Self Cite

View full text Add to dashboard Cite

The high incidence of progressive multifocal leukoencephalopathy (PML) among individuals with acquired immunodeficiency syndrome (AIDS) is similar to the incidence of other immunocompromised diseases. The pathogenic JC virus (JCV) with rearranged regulatory regions (PML-type) causes PML, a demyelinating disease in the brains of immunocompromised patients. In a previous study, Tat protein, encoded by human immunodeficiency virus type 1 (HIV-1), markedly enhanced the expression of a reporter gene under control of the JCV late promoter. In order to examine the enhancement of JCV replication by Tat protein, the neuroblastoma cell line IMR-32 was used because it enables IMR-32-adapted JCV. The extent of JCV replication in IMR-32 cells treated with Tat protein was significantly higher than that in untreated IMR-32 cells. The enhancement of JCV propagation by Tat protein was also examined using IMR-32-derived JCV producing (JCI) cells which continuously produce JCV. Treatment of JCI cells with Tat protein led to a significant increase in the titers of progeny viruses. It has also been shown that Tat protein leads to a decrease in the expression of purine-rich element binding protein α (Purα) as an important mediator of JCV replication in IMR-32 cells. Thus, it is probable that Tat protein enhances JCV replication in IMR-32 cells via the down-regulation of Purα expression and cell proliferation. To our knowledge, this is the first report that exogenous Tat protein enhances the replication of JCV efficiently in neuroblastoma cell lines.

show abstract

Archetype JC virus efficiently propagates in kidney‐derived cells stably expressing HIV‐1 Tat

Cited by 12 publications

References 30 publications

Efficient propagation of archetype BK and JC polyomaviruses

Efficient propagation of archetype BK and JC polyomaviruses

Molecular Biology, Epidemiology, and Pathogenesis of Progressive Multifocal Leukoencephalopathy, the JC Virus-Induced Demyelinating Disease of the Human Brain

Exogenous human immunodeficiency virus‐1 protein, tat, enhances replication of JC virus efficiently in neuroblastoma cell lines

Contact Info

Product

Resources

About