Are Normal Kidney Cells Influenced by Aspirin in Cell Culture?

Abstract

Abstract-Aspirin is today widely used to lessen mild pain and fever, reduce inflammation and prevent heart attacks and strokes. The reports indicate that aspirin also has anticancer effects. This study was exerted to determine the effects of aspirin on proliferation of normal kidney (HEK) cells in cell culture. In this laboratory experimental study, HEK cells were exposed to 10mg/ml, 1mg/ml, 0.1mg/ml, 0.01mg/ml and 0.001mg/ml of aspirin in cell culture. After 48 hours, the viability of HEK cells was examined using MTT assay. The data was analyzed using ANOVA. Our findings show that viability of HEK cells decreased significantly when exposed to 10mg/ml of aspirin. Other doses of aspirin resulted in increased viability. Our findings show that aspirin has anti-proliferative effects against HEK cells only in lower dose.