Are snRNPs involved in splicing?

Lerner, Michael R.; Boyle, John A.; Mount, Stephen M.; Wolin, Sandra L.; Steitz, Joan A.

doi:10.1038/283220a0

Cited by 1,184 publications

(529 citation statements)

References 46 publications

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“…These consensus sequences, termed the donor site or 5h splice site [5h (C\A)AGGU(A\-G)AGU 3h] and acceptor site or 3h splice site [5h (U\C)11N-(U\C)AG(G\A) 3h], signal the precise location for excision by the spliceosome and subsequent removal of an intron [24,25]. The two splice sites overlap the intron\exon boundaries.…”

Section: Post-transcriptional Regulationmentioning

confidence: 99%

Transcriptional control and the role of silencers in transcriptional regulation in eukaryotes

Ogbourne

Antalis

1998

Biochemical Journal

227

171

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Mechanisms controlling transcription and its regulation are fundamental to our understanding of molecular biology and, ultimately, cellular biology. Our knowledge of transcription initiation and integral factors such as RNA polymerase is considerable, and more recently our understanding of the involvement of enhancers and complexes such as holoenzyme and mediator has increased dramatically. However, an understanding of transcriptional repression is also essential for a complete understanding of promoter structure and the regulation of gene expression. Transcriptional repression in eukaryotes is achieved through ‘silencers ’, of which there are two types, namely ‘silencer elements ’ and ‘negative regulatory elements ’ (NREs). Silencer elements are classical, position-independent elements that direct an active repression mechanism, and NREs are position-dependent elements that direct a passive repression mechanism. In addition, ‘repressors ’ are DNA-binding trasncription factors that interact directly with silencers. A review of the recent literature reveals that it is the silencer itself and its context within a given promoter, rather than the interacting repressor, that determines the mechanism of repression. Silencers form an intrinsic part of many eukaryotic promoters and, consequently, knowledge of their interactive role with enchancers and other transcriptional elements is essential for our understanding of gene regulation in eukaryotes.

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Section: Post-transcriptional Regulationmentioning

confidence: 99%

Transcriptional control and the role of silencers in transcriptional regulation in eukaryotes

Ogbourne

Antalis

1998

Biochemical Journal

227

171

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“…For example, Ul RNA has been linked to mRNA splicing (Lerner et al, 1980;Rogers and Wall, 1980) while the RNA moiety of RNAase P has been shown to be absolutely required for its activity (Kole et al, 1980). RNA has even been shown to be capable of making and breaking phosphodiester bonds in the complete absence of protein (Kruger et al, 1982).…”

Section: Introductionmentioning

confidence: 99%

Structure-function relations in E. coli 16S RNA

Thompson¹,

Hearst²

1983

Cell

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“…This RNA forms a characteristic ribonucleoprotein particle which can be immunoprecipitated with anti-RNP or anti-Sm antisera from patients with the autoimmune disease lupus erythematosus (18,19). The sequence of this RNA has been highly conserved in evolution, with only a 25 to 30% difference between Ul RNA from such diverse organisms as rats, drosophila, and sea urchins (3,5,28).…”

mentioning

confidence: 99%

Synthesis of U1 RNA in isolated nuclei from sea urchin embryos: U1 RNA is initiated at the first nucleotide of the RNA.

Morris¹,

Marzluff²

1985

Mol. Cell. Biol.

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Nuclei from sea urchin blastula embryos synthesize a variety of small RNAs, one of which has identical mobility with sea urchin Ul RNA. This RNA is synthesized by RNA polymerase II and, in a hybridizationselection experiment, was selected by the cloned sea urchin Ul gene. The Ul RNA was initiated with ATP, but not GIP, in isolated nuclei with P-S-and y-S-ribonucleotide triphosphates as substrates. The Ul RNA containing thiophosphate at the 5' end was not capped but accumulated as an uncapped transcript from which the thiophosphate could be removed with calf intestinal phosphatase.

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Are snRNPs involved in splicing?

Cited by 1,184 publications

References 46 publications

Transcriptional control and the role of silencers in transcriptional regulation in eukaryotes

Transcriptional control and the role of silencers in transcriptional regulation in eukaryotes

Structure-function relations in E. coli 16S RNA

Synthesis of U1 RNA in isolated nuclei from sea urchin embryos: U1 RNA is initiated at the first nucleotide of the RNA.

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