Are usual immunochemical methods for the determination of human serum transferrin influenced by the iron-saturation of the protein?

Transferrin (TF) is the major transport protein involved in human iron metabolism. The expression of the cell-surface TF receptor is associated with cellular proliferation, the dominant feature of proliferative vitreoretinal disorders with traction retinal detachment. A total of 14 retinal membranes from patients with different clinical diagnoses contained immunoreactive TF. Expression of the cell-surface TF receptor was confirmed by a monoclonal anti-human TF-receptor antibody label in 11 of the 14 specimens. We developed a noncompetitive enzyme-linked immunosorbent assay (ELISA) for TF and found it to be a significant component of vitreal protein, with a level of 65.7 +/- 33.9 mg/l. Vitreal TF as a major iron acceptor probably has a protective function, but its interaction with macrophages and its growth-promoting activity may subsequently stimulate the proliferation of fibroblasts and retinal pigment epithelial cells.

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Transferrin and transferrin receptor expression in intraocular proliferative disease. APAAP-immunolabeling of retinal membranes and ELISA for vitreal transferrin

Weller

Wiedemann

Moter

et al. 1989

Graefe's Arch Clin Exp Ophthalmol

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Immunoturbidimetric assay of transferrin: effect of iron and need for serum blanks

Rifai

King

Malekpour

et al. 1986

Clinical Biochemistry

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An immunoassay for human transferrin

Guindi

Skikne

Covell

et al. 1988

The American Journal of Clinical Nutrition

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Are usual immunochemical methods for the determination of human serum transferrin influenced by the iron-saturation of the protein?

Cited by 4 publications

References 9 publications

Transferrin and transferrin receptor expression in intraocular proliferative disease. APAAP-immunolabeling of retinal membranes and ELISA for vitreal transferrin

Transferrin and transferrin receptor expression in intraocular proliferative disease. APAAP-immunolabeling of retinal membranes and ELISA for vitreal transferrin

Immunoturbidimetric assay of transferrin: effect of iron and need for serum blanks

An immunoassay for human transferrin

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